FastQCFastQC Report
Thu 22 Jan 2015
SE324_GTGGCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE324_GTGGCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20538926
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC1979570.9638137846156124TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG247150.040.58264511
AGTCACG240150.040.5476428
CGTATGC236200.040.1684144
GTATGCC236600.040.15755545
ACGTCTG249850.040.14409615
CGTGGCC235700.040.11998433
GTGGCCA236050.040.0128434
GTCACGT241400.039.82507329
ACACGTC252000.039.73909813
CACACGT252550.039.61691712
GGCCATC239850.039.53837636
CACGTCT255050.039.21977214
CTCGTAT243050.039.0270742
TGGCCAT243850.038.73295235
CAGTCAC255000.038.6980827
CGTCTGA259100.038.63456716
TCGTATG246200.038.6099843
GCCATCT248150.038.26124637
CCAGTCA258350.038.1527426
TCACGTG251300.038.12186430