FastQCFastQC Report
Thu 22 Jan 2015
SE323_CTTGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE323_CTTGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9007119
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2573982.857717323374988TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC289950.043.08130645
AGTCACC293250.042.872728
CGTATGC291350.042.8665644
GCACACG298500.042.80455811
CACACGT298350.042.7054212
CTCGTAT292400.042.65107742
ACGTCTG298300.042.63715415
ACACGTC298700.042.62525613
CACGTCT299000.042.58248514
CGTCTGA299350.042.48055616
TCGTATG295050.042.4586443
CAGTCAC297550.042.4421727
GTCACCT296050.042.4140229
CCTTGTA295700.042.35009433
CCAGTCA298250.042.31238626
AGCACAC303950.042.1184710
ACTCCAG301300.042.0516123
TCCAGTC301900.041.95010825
GTAATCT297650.041.90634537
ACCTTGT299200.041.87724332