FastQCFastQC Report
Thu 22 Jan 2015
SE322_GGCTAC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE322_GGCTAC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8423835
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC710940.8439623995484242TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT87400.039.77229742
TCGTATG89550.039.11889643
CACACGT95100.038.84688612
GTATGCC89800.038.8346145
AGTCACG92600.038.63222528
ACGGCTA92300.038.44090732
CGTATGC90700.038.3996544
CAGTCAC94300.038.07893427
ACACGTC97600.037.92099413
CACGTCT98050.037.74695614
TACATCT92250.037.70567737
GCTACAT93150.037.6312135
TCTCGTA93700.037.0741641
GCACACG100450.036.8450911
GGCTACA95300.036.73502334
ACTCCAG99800.036.61489523
ACGTCTG101400.036.52207615
CCAGTCA99150.036.4431926
CGTCTGA101800.036.26914216
GTCACGG98250.036.2503329
Produced by FastQC (version 0.11.2)