FastQCFastQC Report
Thu 22 Jan 2015
SE321_TAGCTT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE321_TAGCTT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10055684
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2239132.2267306729209073TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCT256350.042.68095432
CACTAGC256500.042.63845431
TAGCTTA257850.042.3454134
TCACTAG259000.042.3224430
CTCGTAT257300.042.24355342
GTATGCC258150.042.16546645
CTAGCTT261050.041.93837433
GTCACTA261850.041.9047629
TCGTATG260550.041.79434243
TCTCGTA260550.041.76843641
CACACGT268900.041.70137812
ACACGTC269150.041.61248813
CGTATGC261750.041.56834844
CAGTCAC266250.041.42350827
AGCTTAT265150.041.22199635
CACGTCT271600.041.1791314
GCACACG272000.041.1764711
AGTCACT267550.041.1381428
ACGTCTG272050.041.11101515
CGTCTGA271700.041.06685316
Produced by FastQC (version 0.11.2)