FastQCFastQC Report
Thu 22 Jan 2015
SE320_GATCAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE320_GATCAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11761302
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1582061.3451401894110022TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG191250.040.92758628
GTATGCC190250.040.7997645
CGTATGC191500.040.48644644
CTCGTAT192900.040.09930442
GCACACG199600.040.0044811
ACACGTC199500.039.889213
GTCACGA195900.039.78383329
TCGTATG195400.039.75897243
ACGTCTG200150.039.73717515
CACGATC195050.039.70343431
CACACGT200900.039.70081312
GATCTCG196000.039.48803339
CGTCTGA201800.039.43607316
CAGTCAC199300.039.4325127
CCAGTCA199450.039.3013326
CACGTCT202750.039.2830814
CGATCAG198800.038.90923333
TCACGAT201600.038.6589930
CAGATCT199000.038.5987837
GAACTCC205100.038.51878721
Produced by FastQC (version 0.11.2)