FastQCFastQC Report
Thu 22 Jan 2015
SE318_GTGAAA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE318_GTGAAA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15486341
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG4915513.1740938676217967TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG585400.043.3491711
ACGTCTG579550.043.31699815
ACACGTC582600.043.2099413
AGTCACG580450.043.13742428
CACACGT588050.043.1155612
CACGTCT583400.043.09284214
TCGTATG578850.042.94571345
GTCACGT580700.042.80502729
CGTCTGA587050.042.7340416
CTCGTAT576100.042.61958344
CCAGTCA586900.042.4640126
CAGTCAC588300.042.45856527
GATCTCG579500.042.4471841
ACGATCT568000.042.43910639
AGCACAC599450.042.43448610
CGATCTC568000.042.419340
ACTCCAG588400.042.35207423
TCCAGTC589050.042.3091525
CACGTGA588750.042.24650231
GAACTCC592900.042.21008721