FastQCFastQC Report
Thu 22 Jan 2015
SE317_GTCCGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE317_GTCCGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30756691
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG3697421.2021514277982634TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC445650.042.1757933
ACGTCCG453050.041.78486632
GTCCGCA452900.041.47083334
TCCGCAC453250.041.2005435
AGTCACG467050.041.19712428
CACGTCC459850.041.1425131
GCACACG475400.040.89947511
TCGTATG472150.040.73307445
GTCACGT469600.040.7290829
CGCACAT463800.040.63203437
ACACGTC479850.040.5717613
ACGTCTG479950.040.5445615
TCACGTC472850.040.23026330
CACACGT484350.040.16694312
CTCGTAT476350.040.16138544
CCGCACA468500.040.0131136
CACGTCT485700.040.00434514
CGTCTGA487800.039.86083616
CAGTCAC486150.039.53228427
CCAGTCA487850.039.0578626
Produced by FastQC (version 0.11.2)