FastQCFastQC Report
Thu 22 Jan 2015
SE316_GTAGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE316_GTAGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11686474
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC2840892.4309214224923616TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG330850.042.29149611
CACACGT332000.042.0704612
ACACGTC332000.041.9959213
ACGTCTG331450.041.91626415
GTATGCC324150.041.72886345
AGTCACG328250.041.72857328
GATCGGA335350.041.6762731
CACGTCT334950.041.55887214
GTCACGT328600.041.4650329
CGTAGAG327200.041.36739333
CGTATGC327150.041.2980744
CGTCTGA336100.041.27733616
TCACGTA329250.041.246530
AGCACAC340600.041.14031610
CACGTAG330600.041.11890431
TCGGAAG344550.040.9813273
CAGTCAC334800.040.945827
CTCGTAT330650.040.88133242
TCGTATG331750.040.813643
GAACTCC340000.040.6746421
Produced by FastQC (version 0.11.2)