FastQCFastQC Report
Thu 22 Jan 2015
SE315_CCGTCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE315_CCGTCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20453829
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG19576399.571014796300489TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2248100.044.3653311
ACACGTC2250500.044.19605313
ACGTCTG2248200.044.1922315
CACACGT2255050.044.184712
CACGTCT2248300.044.16224314
AGCACAC2267300.044.07398210
TCGTATG2199850.044.01906645
CGTCTGA2258050.043.98722516
CGTCCCG2186250.043.92858535
GATCGGA2258750.043.902831
CCGTCCC2191650.043.87373434
CACCCGT2216700.043.8580231
CCCGTCC2203450.043.85524733
GTCCCGA2172750.043.8401436
GTCACCC2225700.043.83533529
TCACCCG2222250.043.8294830
AGAGCAC2286700.043.823058
ACCCGTC2216300.043.8202532
TCGGAAG2292350.043.769533
AGTCACC2236550.043.7423928
Produced by FastQC (version 0.11.2)