FastQCFastQC Report
Thu 22 Jan 2015
SE314_ATGTCA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE314_ATGTCA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15198859
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1613561.0616323238474679TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG205850.040.29809611
ACGTCTG206450.040.2681615
ACACGTC207500.039.98849513
CACACGT208300.039.8673212
CACGTCT208350.039.7929614
CTCGTAT206450.039.33093344
CGTCTGA211500.039.29733316
TCGTATG207450.039.2606445
CAGTCAC213050.038.40820727
CCAGTCA213350.038.21710626
GATCGGA220200.038.175451
ACTCCAG216750.037.65148523
AGCACAC221100.037.6305410
TCCAGTC218000.037.5466625
GAACTCC219150.037.48679721
ATGTCAG215600.037.0564834
ACATGTC216400.037.0442532
TCTCGTA219900.037.03783443
AGTCACA221600.036.69278328
TCGGAAG232550.036.477013
Produced by FastQC (version 0.11.2)