FastQCFastQC Report
Thu 22 Jan 2015
SE313_ACTTGA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE313_ACTTGA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13302291
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1834001.3787098778699098TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT210900.041.6054142
TCGTATG213950.041.2962343
CACACGT220850.041.22850812
CACGTCT221200.041.10224514
CGTATGC215050.040.9594544
CGTCTGA222150.040.93829316
ACACGTC222500.040.90254213
ACGTCTG222500.040.8823215
GTCACAC218450.040.66182729
AGTCACA219950.040.51750628
GTATGCC218850.040.29965245
CAGTCAC222850.040.14167827
TCTCGTA219100.040.05856341
GAACTCC227550.039.8027321
GCACACG229600.039.77488711
GATCGGA227800.039.6562041
CCAGTCA227300.039.39539326
TCGGAAG232650.039.2646643
ACACTTG225900.039.26107432
TCACACT226250.039.2401230
Produced by FastQC (version 0.11.2)