FastQCFastQC Report
Thu 22 Jan 2015
SE312_CAGATC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE312_CAGATC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9655560
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC479880.49699862048394916TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG64900.038.203111
ACGTCTG65550.037.72130215
GTATGCC61950.037.62531345
AGTCACC63600.037.2505728
CGTATGC62850.037.12232244
ACACGTC66700.037.0034713
CACGTCT66650.036.99747514
CACACGT66850.036.9877512
CAGTCAC67550.035.3387927
CTCGTAT66150.035.27041642
CGTCTGA70500.035.07369616
TCGTATG67050.034.83054443
ACTCCAG70000.034.7476423
CCAGTCA69400.034.59127826
GTCACCA69350.034.09713729
GAACTCC71950.034.02569221
GATCGGA73000.033.606461
TCCAGTC71900.033.5136925
CCAGATC69950.033.3543733
AGCACAC75050.033.24624310
Produced by FastQC (version 0.11.2)