FastQCFastQC Report
Thu 22 Jan 2015
SE311_GGTAGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE311_GGTAGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11789772
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC6305685.348432522698488TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG731500.043.54287311
ACGTCTG728900.043.5255215
CACGTCT729350.043.50773614
ACACGTC731350.043.46874213
CACACGT732600.043.42835212
GTCACGG718050.043.38715729
CACGGTA708250.043.374431
GGTAGCA696900.043.2737134
CGGTAGC697950.043.26663233
GTAGCAT697400.043.22655535
AGTCACG724050.043.1798828
CGTCTGA728000.043.17067316
GTATGCC714350.043.12055245
TCACGGT715150.043.09118730
AGCACAC742900.042.96252410
GATCGGA742100.042.927291
TAGCATC701200.042.87999736
CGTATGC718600.042.87178444
CAGTCAC732750.042.8452927
GAACTCC732300.042.78832621