FastQCFastQC Report
Thu 22 Jan 2015
SE310_GAGTGG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE310_GAGTGG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13742886
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC2727181.9844303445433513TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG320250.041.84341428
ACGTCTG327200.041.7110115
GTATGCC317800.041.70582645
CGTATGC318600.041.56579244
GCACACG329500.041.53593411
ACACGTC329400.041.51439313
CGAGTGG311350.041.50755733
CACGTCT329950.041.42473214
GTCACGA321550.041.33139429
TCGTATG321800.041.30627443
ACGAGTG315700.041.2705832
CACACGT331900.041.23558412
CTCGTAT322300.041.20729442
CGTCTGA332550.041.02146516
GGATCTC319350.040.98909838
CAGTCAC329700.040.84198427
CACGAGT319300.040.83345431
CCAGTCA331900.040.62549226
TCACGAG327000.040.56685330
GTGGATC318650.040.40131836
Produced by FastQC (version 0.11.2)