FastQCFastQC Report
Thu 22 Jan 2015
SE309_CGTACG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE309_CGTACG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11577167
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC119350110.309093753247232TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1376900.043.99966411
GATCGGA1377950.043.994591
ACGTCTG1375850.043.89261215
CACACGT1379600.043.88909512
ACACGTC1380050.043.8633713
TCGGAAG1390800.043.851513
CACGTCT1378200.043.84225514
AGCACAC1387150.043.76537310
CGTCTGA1379300.043.73452416
CGGAAGA1393550.043.7245144
AGAGCAC1397700.043.5477038
CCGTACG1327450.043.5099333
CGTACGA1320300.043.46438634
GAAGAGC1406850.043.333246
ACTCCAG1379100.043.3322423
CACCGTA1346800.043.32917831
GAACTCC1382400.043.32218621
GAGCACA1403850.043.3200729
ATCGGAA1408200.043.3144652
GTCACCG1349150.043.2987329
Produced by FastQC (version 0.11.2)