FastQCFastQC Report
Thu 22 Jan 2015
SE308_GTTTCG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE308_GTTTCG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8967527
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC123456813.767095432218937TruSeq Adapter, Index 21 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1373000.044.106221
TCGGAAG1382550.044.0447583
GCACACG1371300.044.03177311
CACACGT1371700.043.9795612
ACACGTC1370750.043.9509613
ACGTCTG1368050.043.92421715
AGCACAC1379100.043.86919810
CGGAAGA1386550.043.862024
CACGTCT1371650.043.8384614
CGTCTGA1371300.043.7867116
AGAGCAC1387600.043.7528888
GTATGCC1351950.043.7200545
ATCGGAA1393300.043.674732
GAAGAGC1394600.043.591356
GAGCACA1394300.043.5216649
GAACTCC1376250.043.4734821
AGTCACG1365500.043.43614628
GGAAGAG1401950.043.42545
ACTCCAG1375400.043.41915523
AAGAGCA1400400.043.3915337
Produced by FastQC (version 0.11.2)