FastQCFastQC Report
Thu 22 Jan 2015
SE307_GTGGCC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE307_GTGGCC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11041038
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC5665515.131320080593872TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG649500.043.4873411
ACGTCTG649100.043.43441815
ACACGTC650150.043.4092613
CACACGT650750.043.39689312
CGTCTGA651000.043.27870616
CACGTCT652000.043.2550314
AGTCACG642250.043.17252728
CGTGGCC623650.043.09644733
GTCACGT635800.043.03369529
GATCGGA657750.043.0308721
GTATGCC640750.043.01375645
GTGGCCA624250.042.96131534
GGCCATC629100.042.94125436
TGGCCAT624600.042.9300435
AGCACAC660300.042.87486610
GAACTCC655600.042.74437321
CGTATGC644650.042.73607644
CTCGTAT645200.042.7345242
TCGGAAG666900.042.7235873
CCAGTCA652050.042.72033726