FastQCFastQC Report
Thu 22 Jan 2015
SE306_GTGAAA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE306_GTGAAA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10867984
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1943931.7886758022463045TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG248600.041.9295328
GTCACGT248500.041.34885429
GCACACG259200.041.2998311
ACGTCTG257200.041.20109615
ACACGTC257200.041.17485413
CACACGT259650.041.13294212
CGTCTGA257600.041.0343916
TCGTATG247200.040.93830545
CACGTCT259000.040.8973914
CGATCTC240200.040.7357140
CTCGTAT246350.040.66857544
GATCTCG246550.040.50782841
ACGATCT242900.040.26437439
CAGTCAC259800.040.25184627
CACGTGA253750.040.24509431
TCACGTG254550.040.22467830
CCAGTCA260500.040.00549726
AACGATC245400.039.99170738
GAACTCC263150.039.85570521
ACTCCAG262300.039.80269623
Produced by FastQC (version 0.11.2)