FastQCFastQC Report
Thu 22 Jan 2015
SE305_GCCAAT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE305_GCCAAT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110181
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1289071.4149773753123016TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC149000.042.3854429
TCACGCC149900.041.7707430
ACGTCTG157550.041.42761215
CACGTCT158550.041.20889714
CACACGT159500.041.2032512
GCACACG159850.041.18340711
ACACGTC159000.041.17716613
CACGCCA152300.041.14204831
CGTATGC156250.041.09552444
GTATGCC156550.041.03114345
AGTCACG155300.040.9702428
CTCGTAT158000.040.56915342
CGTCTGA161000.040.51415616
CAGTCAC159100.040.18967427
TCGTATG159950.040.15896243
ACGCCAA157150.039.9152632
CCAGTCA161100.039.7326326
ACTCCAG162300.039.64853723
GAACTCC163450.039.60576621
AGCACAC166600.039.56883210
Produced by FastQC (version 0.11.2)