FastQCFastQC Report
Thu 22 Jan 2015
SE304_ACAGTG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE304_ACAGTG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096620
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1710121.6937549397719236TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG201100.042.16739311
GTATGCC198100.042.02231245
ACACGTC202300.041.9506313
ACGTCTG201700.041.94156315
CGTATGC198950.041.83146344
CACACGT203500.041.6590312
CACGTCT203200.041.62088814
GTCACAC197200.041.2671329
CTCGTAT201250.041.21923442
CGTCTGA205100.041.21562616
TCGTATG203300.040.95853443
CAGTCAC201100.040.89196827
ACTCCAG202800.040.85115423
CCAGTCA202250.040.69282526
AGCACAC208900.040.6898610
GATCTCG203150.040.667639
TCCAGTC202900.040.62899825
GATCGGA209400.040.5412861
CACAGTG199600.040.50040433
GAACTCC206350.040.49949321
Produced by FastQC (version 0.11.2)