FastQCFastQC Report
Thu 22 Jan 2015
SE303_TGACCA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE303_TGACCA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18916355
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1491660.7885557233409924TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG192600.039.90455211
ACGTCTG194800.039.30373815
GTATGCC188850.039.12433645
CGTATGC188800.039.0989544
ACACGTC196700.039.01559413
CACGTCT196800.038.92717414
CACACGT197600.038.8834412
CGTCTGA200250.038.24716
CTCGTAT196250.037.75225442
CAGTCAC197250.037.68633327
CCAGTCA199150.037.5640326
TCGTATG197450.037.55700343
ACTGACC192650.037.41832732
ACTCCAG202550.037.01260823
GAACTCC205100.036.8831921
CTGACCA194900.036.8709233
GTCACTG201500.036.60115429
AGCACAC211800.036.46772810
TCCAGTC205150.036.432525
CTCCAGT211850.035.4619524
Produced by FastQC (version 0.11.2)