FastQCFastQC Report
Thu 22 Jan 2015
SE302_CTTGTA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE302_CTTGTA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16845466
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC251823214.949019516586837TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2766000.044.33333645
CACGTCT2804400.044.187614
CGTCTGA2802750.044.16366616
CACACGT2813700.044.15669612
ACACGTC2812550.044.1315513
ACGTCTG2809500.044.10819215
AGTCACC2767200.044.0978428
GTAATCT2746050.044.0638737
GCACACG2824950.044.04535711
CCAGTCA2788350.044.0191426
CAGTCAC2791000.044.01603727
CTCCAGT2793550.044.01044524
CTCGTAT2781750.044.01034542
ACTCCAG2798700.043.99844723
CCTTGTA2751100.043.98707233
TAATCTC2756050.043.9823638
CGTATGC2787950.043.9721944
CTTGTAA2747600.043.97104634
TCGTATG2792000.043.96078543
TCTGAAC2813400.043.9395218
Produced by FastQC (version 0.11.2)