FastQCFastQC Report
Thu 22 Jan 2015
SE301_GGCTAC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE301_GGCTAC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11703694
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1725531.4743464755657487TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA208150.041.49577332
AGTCACG211900.041.41971628
CTCGTAT209200.041.2659942
GCTACAT208150.040.81480435
CGTATGC212650.040.75520344
CACACGT221750.040.73653412
GTATGCC212750.040.7254745
TCGTATG213300.040.7048443
ACACGTC223050.040.5697213
ACGTCTG222250.040.5234115
GGCTACA211500.040.27470834
CACGGCT215750.040.07575631
CAGTCAC219850.040.05497727
CACGTCT225450.039.99812714
GCACACG226650.039.8657711
CGGCTAC214150.039.8603833
TCTCGTA216950.039.83334741
CGTCTGA226500.039.7741616
CCAGTCA221900.039.7254926
GTCACGG220650.039.64463829
Produced by FastQC (version 0.11.2)