Basic Statistics
Measure | Value |
---|---|
Filename | SE300_TAGCTT_L005_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14586632 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC | 572384 | 3.924031263694045 | TruSeq Adapter, Index 10 (100% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACTAGC | 63355 | 0.0 | 43.517384 | 31 |
ACTAGCT | 63690 | 0.0 | 43.284954 | 32 |
GTATGCC | 64850 | 0.0 | 43.18376 | 45 |
TCACTAG | 64370 | 0.0 | 43.01644 | 30 |
TAGCTTA | 63985 | 0.0 | 43.015064 | 34 |
ACGTCTG | 66125 | 0.0 | 43.0146 | 15 |
ACACGTC | 66330 | 0.0 | 43.010555 | 13 |
CACGTCT | 66205 | 0.0 | 42.99321 | 14 |
CGTATGC | 65145 | 0.0 | 42.97785 | 44 |
GCACACG | 66380 | 0.0 | 42.967987 | 11 |
CTAGCTT | 64140 | 0.0 | 42.9497 | 33 |
CACACGT | 66470 | 0.0 | 42.90304 | 12 |
CTCGTAT | 65200 | 0.0 | 42.893288 | 42 |
TCGTATG | 65555 | 0.0 | 42.808586 | 43 |
CGTCTGA | 66410 | 0.0 | 42.760174 | 16 |
GCTTATC | 64485 | 0.0 | 42.681538 | 36 |
CCAGTCA | 65800 | 0.0 | 42.673126 | 26 |
CAGTCAC | 65745 | 0.0 | 42.66092 | 27 |
TATCTCG | 65685 | 0.0 | 42.42586 | 39 |
CTTATCT | 65135 | 0.0 | 42.38341 | 37 |