FastQCFastQC Report
Thu 22 Jan 2015
SE300_TAGCTT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE300_TAGCTT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14586632
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC5723843.924031263694045TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC633550.043.51738431
ACTAGCT636900.043.28495432
GTATGCC648500.043.1837645
TCACTAG643700.043.0164430
TAGCTTA639850.043.01506434
ACGTCTG661250.043.014615
ACACGTC663300.043.01055513
CACGTCT662050.042.9932114
CGTATGC651450.042.9778544
GCACACG663800.042.96798711
CTAGCTT641400.042.949733
CACACGT664700.042.9030412
CTCGTAT652000.042.89328842
TCGTATG655550.042.80858643
CGTCTGA664100.042.76017416
GCTTATC644850.042.68153836
CCAGTCA658000.042.67312626
CAGTCAC657450.042.6609227
TATCTCG656850.042.4258639
CTTATCT651350.042.3834137