FastQCFastQC Report
Thu 22 Jan 2015
SE299_GATCAG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE299_GATCAG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16383933
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC13684208.352207006705898TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1511400.044.03468345
CACACGT1539650.043.8623912
AGTCACG1518800.043.81717328
CACGTCT1539750.043.7557914
ACGTCTG1539800.043.7529115
CGTCTGA1539100.043.7377416
ACACGTC1542450.043.73462713
GCACACG1547700.043.7025711
CTCGTAT1518900.043.6676442
TCGTATG1527250.043.64986843
CAGTCAC1529900.043.60661727
CGTATGC1526200.043.60029644
CCAGTCA1528250.043.57861326
CTCCAGT1531050.043.51867724
AGCACAC1555050.043.5104810
CAGATCT1494300.043.41688537
TCTCGTA1527650.043.41016441
GTCACGA1517650.043.40711229
TCCAGTC1536550.043.40032225
GAACTCC1544900.043.35003721
Produced by FastQC (version 0.11.2)