FastQCFastQC Report
Thu 22 Jan 2015
SE298_ACTTGA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE298_ACTTGA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17713599
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC16930759.55805198028927TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1858600.044.1047445
TCCAGTC1880050.043.7140325
CGTATGC1874250.043.71245244
GTCACAC1860400.043.70530329
CAGTCAC1881200.043.68252627
CCAGTCA1879900.043.6672526
CTCGTAT1877150.043.60297442
TCGTATG1882800.043.59282743
CTCCAGT1889600.043.56281324
AGTCACA1875800.043.5204128
GAACTCC1902450.043.51857821
ACTCCAG1896950.043.47541823
ACGTCTG1917400.043.41178515
CGTCTGA1917000.043.37362716
TCTCGTA1889750.043.3527341
ACACTTG1875450.043.32578732
CACGTCT1924350.043.29942314
AATCTCG1885800.043.26218839
ACACGTC1931350.043.2553713
ATCTCGT1896400.043.21494340
Produced by FastQC (version 0.11.2)