FastQCFastQC Report
Thu 22 Jan 2015
SE297_CAGATC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE297_CAGATC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17087498
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1705260.9979576881296489TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC209950.040.4649744
AGTCACC206000.040.3452828
GTATGCC211750.040.17411845
ACACGTC218300.039.79322413
CTCGTAT214500.039.69053342
GCACACG219600.039.6601111
CACGTCT219400.039.54255714
CACACGT221300.039.34527612
ACGTCTG220300.039.28909715
TCGTATG217300.039.21016743
CAGTCAC216350.039.0183627
CGTCTGA225400.038.42188316
GTCACCA216550.038.2446529
CCAGTCA221650.038.2173326
GAACTCC226300.038.07156821
TCCAGTC224900.037.8251225
CCAGATC217050.037.74191733
ACCAGAT218900.037.66962432
ACTCCAG227900.037.56556323
CATCTCG221850.037.44254739
Produced by FastQC (version 0.11.2)