FastQCFastQC Report
Thu 22 Jan 2015
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276776
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC114440.5026405759723398No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG76450.335781824825982No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG52650.2312480454818568No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT51180.22479154734589613No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA45850.2013812513835353No Hit
ATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCC34000.14933397049160743No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC32070.14085707157840735No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA29310.128734666914971No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG26970.11845697600466625No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT25020.1098922335794123No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC22920.10066866481375418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT12850.040.6519781
GCACACG6350.036.47356811
ACGTCTG6300.036.4061215
AGCACAC6500.035.9778110
CACACGT6400.035.83727312
ACACGTC6700.034.2326213
CGTCTGA6700.034.2326216
CACGTCT6800.034.0598814
TCGTTGG16750.032.256834
AGAGCAC7300.032.0350348
ATTCGTT16750.031.321752
CAGTCAC5600.030.9184327
GTCTCCC21700.030.87957442
ATTCGGT21800.030.6381424
GAATTCC17650.030.57604210
TTCGGTA21700.030.5707225
CAGGCGG21800.030.5316336
AATTCGG22050.030.39275623
GGTATTC22050.030.3894228
CCCTTTA2750.030.25606344
Produced by FastQC (version 0.11.2)