FastQCFastQC Report
Thu 22 Jan 2015
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536196
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAC1400.028.9234529
ACCGATC1550.024.67305234
AGACCGA1650.023.17771532
CGAGACC1800.022.4960230
ACCGGTT500.002267689422.49601733
GAGACCG1850.021.88801831
CGATCTC1850.020.67201836
CCGATCT2403.6379788E-1215.9346835
AGGAATG2550.015.88110722
AGCGGTT2550.015.87954211
AGATCGG2557.2759576E-1215.0266741
GATCTCG2801.8189894E-1214.46172537
GGTTCAG2801.8189894E-1214.46172514
GTGTTTG8550.013.4449193
TGTGTTT9300.012.1182842
CGGTACT1350.004014668511.66460245
TTGTGTT9400.011.5097931
ACGGTAC1450.006837379710.86014744
TCGGAAG3754.4383341E-1010.8134384
TGTTTGA11800.010.1239031
Produced by FastQC (version 0.11.2)