FastQCFastQC Report
Thu 22 Jan 2015
foxfnk412hpf2_AAGACG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefoxfnk412hpf2_AAGACG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8543274
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG102700.013.1066153
TTGTGTT103650.012.8127561
TGTGTTT106000.012.4437582
CTACCCG9350.011.55125816
GAGGCTA9150.011.06497512
GCTACCC10750.010.46473215
TGTTTGA141900.010.3424871
GACGGGC13500.09.3328279
AGGCTAC11000.09.20404613
TGACGGG16750.08.9995128
ACGGGCG13850.08.93453410
TTTGTGT63300.08.5698482
CTAAGCG12950.08.51305241
GTTTGAG76600.08.3160562
TAACTTT53000.08.1118011
TACTAAG15000.07.79957739
TAAGCGG15000.07.79957742
CCGCTGA15250.07.67261320
AGGGACG21300.07.605221332
CGGTGTG18550.07.39865115
Produced by FastQC (version 0.11.2)