FastQCFastQC Report
Thu 22 Jan 2015
foxfnk412hpf1_CCTTCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefoxfnk412hpf1_CCTTCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9672301
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG111300.012.6395253
TTGTGTT113300.012.5554721
TGTGTTT118200.011.8635992
TGTTTGA143250.09.7713894
GACGGGC12350.08.3801679
GTTTGAG88250.07.83014062
ACGGGCG13000.07.788089810
TTTGAGG33150.07.604673
TGACGGG18650.07.60017358
TTTGTGT70400.06.7141732
TTTGAGC24950.06.5856443
CGGTGTG16800.06.562220615
TTGACGG22650.06.5559787
CTACCCG5856.475264E-66.538757316
GAGGCTA4901.9291017E-46.42826412
TAACTTT69900.06.2791861
TTTGAGT28050.06.1787953
AACTTTG69450.06.15782362
GCGGTGT17350.06.0948114
AGGGACG17950.06.01642532