##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 mx24-2.3420000000ce23.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19536313 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41211568426448 33.0 33.0 33.0 33.0 33.0 2 32.373157667979626 33.0 33.0 33.0 33.0 33.0 3 32.35842464235703 33.0 33.0 33.0 33.0 33.0 4 36.1976674923257 37.0 37.0 37.0 37.0 37.0 5 36.18606335801438 37.0 37.0 37.0 37.0 37.0 6 36.17111012707464 37.0 37.0 37.0 37.0 37.0 7 36.221946638549454 37.0 37.0 37.0 37.0 37.0 8 36.21086977875508 37.0 37.0 37.0 37.0 37.0 9 36.205429089920905 37.0 37.0 37.0 37.0 37.0 10-11 36.182819424524986 37.0 37.0 37.0 37.0 37.0 12-13 36.17807267420419 37.0 37.0 37.0 37.0 37.0 14-15 38.365737025200204 40.0 37.0 40.0 37.0 40.0 16-17 38.3490610024522 40.0 37.0 40.0 37.0 40.0 18-19 38.3246979611762 40.0 37.0 40.0 37.0 40.0 20-21 38.28805304767589 40.0 37.0 40.0 37.0 40.0 22-23 38.252528970026226 40.0 37.0 40.0 37.0 40.0 24-25 38.215494320755404 40.0 37.0 40.0 37.0 40.0 26-27 38.16376201589317 40.0 37.0 40.0 37.0 40.0 28-29 38.11687197579195 40.0 37.0 40.0 37.0 40.0 30-31 38.068704289289386 40.0 37.0 40.0 37.0 40.0 32-33 37.98280008105931 40.0 37.0 40.0 33.0 40.0 34-35 37.904137029336084 40.0 37.0 40.0 33.0 40.0 36-37 37.830306005027666 40.0 37.0 40.0 33.0 40.0 38-39 37.73360039839657 40.0 37.0 40.0 33.0 40.0 40-41 37.61500806216608 40.0 37.0 40.0 33.0 40.0 42-43 37.51585160925708 40.0 37.0 40.0 33.0 40.0 44-45 37.39564927118029 40.0 37.0 40.0 33.0 40.0 46-47 37.2696411805032 40.0 37.0 40.0 33.0 40.0 48-49 37.11409588902471 40.0 37.0 40.0 33.0 40.0 50-51 35.67849120251093 38.5 35.0 38.5 30.0 38.5 52-53 36.004391053726465 37.0 37.0 38.5 33.0 40.0 54-55 36.69072557344879 37.0 37.0 40.0 33.0 40.0 56-57 36.65224676734039 37.0 37.0 40.0 33.0 40.0 58-59 36.51523926239307 37.0 37.0 40.0 33.0 40.0 60-61 36.33486625649374 37.0 37.0 40.0 33.0 40.0 62-63 36.15067774047232 37.0 37.0 40.0 33.0 40.0 64-65 35.95102765808471 37.0 37.0 40.0 33.0 40.0 66-67 35.73965970446932 37.0 37.0 38.5 33.0 40.0 68-69 35.53066745501057 37.0 37.0 37.0 33.0 40.0 70-71 35.31619955618033 37.0 37.0 37.0 33.0 40.0 72-73 35.110971322992214 37.0 33.0 37.0 33.0 40.0 74-75 34.88770670289732 37.0 33.0 37.0 27.0 37.0 76-77 34.70380334815479 37.0 33.0 37.0 27.0 37.0 78-79 34.53037643285097 37.0 33.0 37.0 27.0 37.0 80-81 34.37882355283722 37.0 33.0 37.0 27.0 37.0 82-83 34.268137467904 37.0 33.0 37.0 27.0 37.0 84-85 34.16959661221644 37.0 33.0 37.0 27.0 37.0 86-87 34.06530070438572 37.0 33.0 37.0 27.0 37.0 88-89 33.9811255327451 37.0 33.0 37.0 27.0 37.0 90-91 33.89476709346334 37.0 33.0 37.0 27.0 37.0 92-93 33.81779827135243 37.0 33.0 37.0 27.0 37.0 94-95 33.73576690238327 37.0 33.0 37.0 27.0 37.0 96-97 33.64358781516246 37.0 33.0 37.0 27.0 37.0 98-99 33.57637439060277 37.0 33.0 37.0 27.0 37.0 100-101 31.777222498431513 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1987629743033068 2306 2 0.28890005884351666 2306 3 0.3343954098731885 2306 4 0.4288682629733742 2306 5 0.4126894188713095 2306 6 0.46029337519026825 2306 7 0.4430857199519025 2306 8 0.4547432390121884 2306 9 0.48251064862485293 2306 10-11 0.47205273905390754 2306 12-13 0.4613298957063918 2306 14-15 0.783687282534629 2306 16-17 0.7979755064505625 2306 18-19 0.8200022606636779 2306 20-21 0.8065488069371298 2306 22-23 0.8018160930985161 2306 24-25 0.8060183724557604 2306 26-27 0.8494921559734578 2306 28-29 0.8536670268098803 2306 30-31 0.8287667556769591 2306 32-33 0.8898690421665663 2306 34-35 0.8467286723384078 2306 36-37 0.8722109724766298 2306 38-39 0.8436913394219445 2306 40-41 0.9145925166623101 2306 42-43 0.8723494184747338 2306 44-45 0.8783501641360587 2306 46-47 0.8864692671042533 2306 48-49 0.8718602017411641 2306 50-51 0.7803716085740646 2306 52-53 0.8428247647184506 2306 54-55 0.9009160580470947 2306 56-57 0.8956474306167976 2306 58-59 0.9405471225731006 2306 60-61 0.881305186621745 2306 62-63 0.8895651137075191 2306 64-65 0.8894577578250349 2306 66-67 0.8756310795221935 2306 68-69 0.929317661396766 2306 70-71 0.918591863239719 2306 72-73 0.904000677831803 2306 74-75 0.902283021638361 2306 76-77 0.9255876794844653 2306 78-79 0.8900231433982384 2306 80-81 0.9094808686448346 2306 82-83 0.9411127739085074 2306 84-85 0.9648970875540357 2306 86-87 0.9519018504414092 2306 88-89 0.9503088266274133 2306 90-91 0.9953690064108471 2306 92-93 1.0022366229964206 2306 94-95 1.0045551965449064 2306 96-97 0.9308521983773304 2306 98-99 0.9247954306353563 2306 100-101 0.8292886267691912 2307 1 0.2133523138767046 2307 2 0.2569200571716834 2307 3 0.2867618801277416 2307 4 0.37948103761722507 2307 5 0.41724236824945393 2307 6 0.3921428872395012 2307 7 0.39221261953669995 2307 8 0.3724727947226967 2307 9 0.39108541605414615 2307 10-11 0.38295442946858316 2307 12-13 0.40835536400252437 2307 14-15 0.6925692979235833 2307 16-17 0.6796263618737584 2307 18-19 0.673396365220853 2307 20-21 0.6924338876485834 2307 22-23 0.6924965827309038 2307 24-25 0.7022358802694626 2307 26-27 0.726760864177713 2307 28-29 0.7266239802977452 2307 30-31 0.723760985226285 2307 32-33 0.7193785306356517 2307 34-35 0.7516863352430079 2307 36-37 0.7454323552519497 2307 38-39 0.7024090747125555 2307 40-41 0.7725154341319183 2307 42-43 0.7739845270222148 2307 44-45 0.8229187321154328 2307 46-47 0.7524757569178391 2307 48-49 0.7834689520118445 2307 50-51 0.6671638999983216 2307 52-53 0.7572356154592299 2307 54-55 0.8461000093794055 2307 56-57 0.783959624993166 2307 58-59 0.7976692983141618 2307 60-61 0.7730198937007202 2307 62-63 0.8004846565206165 2307 64-65 0.7751680976702744 2307 66-67 0.7751617400829502 2307 68-69 0.7787947722535833 2307 70-71 0.7550377288149832 2307 72-73 0.7511548735609068 2307 74-75 0.73555251161401 2307 76-77 0.7163418785466433 2307 78-79 0.7866568287672777 2307 80-81 0.7998893800414351 2307 82-83 0.7865977444537222 2307 84-85 0.783776285283615 2307 86-87 0.8463011623553456 2307 88-89 0.7659075683525671 2307 90-91 0.7683880289654113 2307 92-93 0.8106210364903319 2307 94-95 0.813565749202489 2307 96-97 0.8602923944158007 2307 98-99 0.7970854489445003 2307 100-101 0.7870456223882485 2201 1 0.25114014339391844 2201 2 0.288293228508536 2201 3 0.3260318296066558 2201 4 0.40771863967108146 2201 5 0.40714553880805227 2201 6 0.4309732848271324 2201 7 0.3799577866795545 2201 8 0.36229609429841503 2201 9 0.42381576858578285 2201 10-11 0.39908899096107575 2201 12-13 0.43961264089226404 2201 14-15 0.6828871100402765 2201 16-17 0.6988730743211917 2201 18-19 0.6491032966096313 2201 20-21 0.6972068582251225 2201 22-23 0.7399353090408809 2201 24-25 0.7470298403332478 2201 26-27 0.7488695039626165 2201 28-29 0.7177232647131078 2201 30-31 0.7627088256649799 2201 32-33 0.7969686478356337 2201 34-35 0.8081120263142836 2201 36-37 0.8141191086863557 2201 38-39 0.7844472770887805 2201 40-41 0.7779952382965476 2201 42-43 0.8169519190243548 2201 44-45 0.8379684751308716 2201 46-47 0.8298700727976751 2201 48-49 0.8480371508821634 2201 50-51 0.7626241908379114 2201 52-53 0.8372292914083133 2201 54-55 0.9022240129103167 2201 56-57 0.8901737530687157 2201 58-59 0.8215384836516151 2201 60-61 0.8704694247427369 2201 62-63 0.8791168803461176 2201 64-65 0.9067171651329673 2201 66-67 0.8996926409782589 2201 68-69 0.851469368296911 2201 70-71 0.8534803148909802 2201 72-73 0.8721795777666586 2201 74-75 0.9310066713891914 2201 76-77 0.963862354432905 2201 78-79 0.9657526980597879 2201 80-81 0.9489124758348808 2201 82-83 0.9197040884323897 2201 84-85 0.9484711774368435 2201 86-87 0.9582551956971841 2201 88-89 0.9834903048953834 2201 90-91 0.9784293204382948 2201 92-93 1.0010920580513272 2201 94-95 1.0069341934671527 2201 96-97 0.9549567646045674 2201 98-99 0.9930776944759572 2201 100-101 0.9433489880088359 1101 1 0.2706601444576009 1101 2 0.29045586146691704 1101 3 0.26680009494892687 1101 4 0.3716869330039003 1101 5 0.3651086352498254 1101 6 0.39261011793077216 1101 7 0.3571870998433866 1101 8 0.36228848167389316 1101 9 0.3839512843521433 1101 10-11 0.39295654866619856 1101 12-13 0.3813468355236438 1101 14-15 0.6251266565912417 1101 16-17 0.6514981767100849 1101 18-19 0.6469386823762804 1101 20-21 0.6554590192889052 1101 22-23 0.6687000686450233 1101 24-25 0.6755815302303674 1101 26-27 0.6459350844721499 1101 28-29 0.6453234807033255 1101 30-31 0.6723038124840812 1101 32-33 0.7186016598636726 1101 34-35 0.7346393558672801 1101 36-37 0.7064617961101902 1101 38-39 0.6539005823232387 1101 40-41 0.7374604627000991 1101 42-43 0.7363128819006235 1101 44-45 0.7446035435638336 1101 46-47 0.7295993246665162 1101 48-49 0.8087561555243994 1101 50-51 0.7058293428810387 1101 52-53 0.7383321736693276 1101 54-55 0.7395256973617705 1101 56-57 0.695159891706723 1101 58-59 0.7656780796941476 1101 60-61 0.7816818361411322 1101 62-63 0.7830170194501918 1101 64-65 0.7601440458019724 1101 66-67 0.7596875138462025 1101 68-69 0.7427532397322949 1101 70-71 0.7901676066527514 1101 72-73 0.7714916886063321 1101 74-75 0.7557899540983399 1101 76-77 0.8075033353436183 1101 78-79 0.8637497268809682 1101 80-81 0.8371300355502598 1101 82-83 0.8201413121175634 1101 84-85 0.8119876091985674 1101 86-87 0.8187168026051168 1101 88-89 0.8099047860074364 1101 90-91 0.7751992521528237 1101 92-93 0.7757454089742168 1101 94-95 0.7760100062678887 1101 96-97 0.7834497639290774 1101 98-99 0.8183994311147842 1101 100-101 0.822312073464154 2304 1 0.15490396677205354 2304 2 0.2890172517663743 2304 3 0.30798597292930197 2304 4 0.3936664897629214 2304 5 0.40125836015778304 2304 6 0.41061845699348254 2304 7 0.3382236506269862 2304 8 0.38302881186759663 2304 9 0.3595182704912716 2304 10-11 0.4010650407542826 2304 12-13 0.4199322440716955 2304 14-15 0.6801361513893696 2304 16-17 0.6839158007673376 2304 18-19 0.651095296078509 2304 20-21 0.6727249086750007 2304 22-23 0.6957300365719377 2304 24-25 0.708599493003959 2304 26-27 0.7386496163662173 2304 28-29 0.7647296325137631 2304 30-31 0.7924053401951383 2304 32-33 0.8040348730309503 2304 34-35 0.7914768315442302 2304 36-37 0.8264084079114369 2304 38-39 0.795101146669495 2304 40-41 0.8092706531398193 2304 42-43 0.7985866317512489 2304 44-45 0.8196961892919106 2304 46-47 0.8204397682717897 2304 48-49 0.8015037318615796 2304 50-51 0.7684698849952198 2304 52-53 0.8980661111814214 2304 54-55 0.9415556012639286 2304 56-57 0.8448133125595305 2304 58-59 0.8771997972578092 2304 60-61 0.8681318821962662 2304 62-63 0.8942927309135698 2304 64-65 0.9197859843078859 2304 66-67 0.9290471908837006 2304 68-69 0.9244980485793874 2304 70-71 0.9099081331026895 2304 72-73 0.9332793218086195 2304 74-75 0.9257715584215518 2304 76-77 0.8997899589164078 2304 78-79 0.9389987248792764 2304 80-81 0.955396489303709 2304 82-83 0.960317741877212 2304 84-85 0.9594879737080007 2304 86-87 1.0138135265417532 2304 88-89 1.0068192948219021 2304 90-91 0.9735698300006774 2304 92-93 1.0025526623805519 2304 94-95 1.0213658740870812 2304 96-97 1.0767528971486797 2304 98-99 1.0379936732459782 2304 100-101 1.157311520165642 2202 1 0.21714761367206137 2202 2 0.24183864338573358 2202 3 0.24598611765116374 2202 4 0.276224760851548 2202 5 0.300674971204721 2202 6 0.29115740574597737 2202 7 0.29239661946003537 2202 8 0.287548538051567 2202 9 0.2279240135431877 2202 10-11 0.24756402643337339 2202 12-13 0.25859091991697625 2202 14-15 0.45809555571062077 2202 16-17 0.45628055292446135 2202 18-19 0.43640419539573116 2202 20-21 0.4828502963521757 2202 22-23 0.4331029700582789 2202 24-25 0.4512741396185902 2202 26-27 0.4320253555984408 2202 28-29 0.5268083281393245 2202 30-31 0.5036587570784619 2202 32-33 0.504747951758894 2202 34-35 0.5145336469244768 2202 36-37 0.4926348880479736 2202 38-39 0.5307494698707771 2202 40-41 0.5036654095426343 2202 42-43 0.4678076169403482 2202 44-45 0.5051022108938454 2202 46-47 0.48066938550925187 2202 48-49 0.41995560272432186 2202 50-51 0.46464220073384865 2202 52-53 0.5374630673200116 2202 54-55 0.5773111695714022 2202 56-57 0.5924933332969147 2202 58-59 0.5395786822125856 2202 60-61 0.567866449239844 2202 62-63 0.5631291990047629 2202 64-65 0.5685176708508806 2202 66-67 0.5382657238750852 2202 68-69 0.5531603631631228 2202 70-71 0.5012844325184602 2202 72-73 0.5835748848664224 2202 74-75 0.5663882252880299 2202 76-77 0.5604406814698137 2202 78-79 0.578780356348247 2202 80-81 0.5923112261579178 2202 82-83 0.5846768389677948 2202 84-85 0.5788399784333862 2202 86-87 0.5733261457544572 2202 88-89 0.6304018639887161 2202 90-91 0.5480474340899022 2202 92-93 0.5828017130008831 2202 94-95 0.5871327517567622 2202 96-97 0.5939192914019031 2202 98-99 0.5721970742421547 2202 100-101 0.6445546046303221 1102 1 0.11645386740230634 1102 2 0.14295361149005004 1102 3 0.17843179628432893 1102 4 0.18795613035911174 1102 5 0.18597548461936952 1102 6 0.21124237866968798 1102 7 0.2124250448370404 1102 8 0.21444182823370994 1102 9 0.20479530892085762 1102 10-11 0.1975960166443187 1102 12-13 0.18084793732452198 1102 14-15 0.3372587775373219 1102 16-17 0.33249566229635974 1102 18-19 0.31142793486971243 1102 20-21 0.33586453285534645 1102 22-23 0.3427851692244772 1102 24-25 0.37342616382803584 1102 26-27 0.3525984600434171 1102 28-29 0.39444168595020557 1102 30-31 0.3261589387573238 1102 32-33 0.40520921813178035 1102 34-35 0.3505204706647973 1102 36-37 0.4340483858301454 1102 38-39 0.423416149795095 1102 40-41 0.40180915330816447 1102 42-43 0.39243113506071836 1102 44-45 0.409062280764104 1102 46-47 0.3534517218307087 1102 48-49 0.3703858747442368 1102 50-51 0.444209716344119 1102 52-53 0.41047184087473454 1102 54-55 0.5241196902978942 1102 56-57 0.5246241673673993 1102 58-59 0.48463144243012124 1102 60-61 0.4842750367824209 1102 62-63 0.4640329731008421 1102 64-65 0.4352951247983583 1102 66-67 0.43710837273084024 1102 68-69 0.41152797917551 1102 70-71 0.4841936822682271 1102 72-73 0.4748367836045162 1102 74-75 0.4759891584545173 1102 76-77 0.44407411637145344 1102 78-79 0.4726061029317634 1102 80-81 0.475841472374519 1102 82-83 0.48406521185187756 1102 84-85 0.48724482787194034 1102 86-87 0.41976595045213116 1102 88-89 0.4125342647325212 1102 90-91 0.41848460929290354 1102 92-93 0.41911353322424816 1102 94-95 0.47431288800854077 1102 96-97 0.5062952848354385 1102 98-99 0.517878385023252 1102 100-101 0.5259308883203957 2305 1 0.17899301685789482 2305 2 0.1870621149480698 2305 3 0.2126099194143336 2305 4 0.20423676910157695 2305 5 0.2553195770237693 2305 6 0.26220124825865554 2305 7 0.24643791894449407 2305 8 0.26481788381448723 2305 9 0.22075737787670846 2305 10-11 0.257315245768055 2305 12-13 0.2604872704822938 2305 14-15 0.3977020247878045 2305 16-17 0.3434926608381019 2305 18-19 0.36106306130107413 2305 20-21 0.3944445256726894 2305 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1047.0 5 859.0 6 780.0 7 1362.0 8 2400.0 9 4406.0 10 8434.0 11 13696.0 12 18266.0 13 22478.0 14 26042.0 15 29719.0 16 33540.0 17 38202.0 18 43752.0 19 49107.0 20 55029.0 21 61378.0 22 68828.0 23 77814.0 24 89210.0 25 102401.0 26 118752.0 27 140302.0 28 166747.0 29 201619.0 30 250408.0 31 317750.0 32 414833.0 33 555497.0 34 768049.0 35 1190102.0 36 2486059.0 37 6403531.0 38 5753650.0 39 3717.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.775760679682882 12.60235268222743 11.358145340911234 56.26374129717845 2 23.05118321307374 18.93274627985236 38.58920687789875 19.426863629175152 3 23.889691702250204 24.38216416144838 24.55678503322274 27.171359103078675 4 26.528715258031948 28.56439236060759 17.9594457170024 26.94744666435806 5 28.82496071818365 30.45590986595178 21.19527385531719 19.523855560547382 6 20.848499864691124 33.84750192972689 22.138283926894065 23.165714278687915 7 19.55684176525877 15.068390393987205 41.04327989417924 24.331487946574782 8 21.13914363296688 20.37818852190008 27.289558758540455 31.193109086592585 9 21.789438966625138 19.668550252108144 31.068920901710417 27.4730898795563 10-11 25.174166400582397 28.218331777813045 21.056716357503007 25.55078546410155 12-13 23.07600510418849 22.673949597137646 27.61247070314351 26.637574595530356 14-15 23.865002603062504 24.32638854452608 26.10015108397724 25.708457768434172 16-17 25.14385124976984 24.519562798706403 24.68394958302992 25.65263636849384 18-19 24.61550773427929 24.920100844112287 24.70250505122566 25.761886370382765 20-21 24.657301065985383 24.9718808502542 25.141249265242415 25.229568818518004 22-23 24.675643556602168 24.944154772187556 25.01020493309849 25.369996738111787 24-25 24.376416992454246 25.0693403781868 25.086022294121367 25.46822033523759 26-27 24.480731010129837 25.120778925327564 25.241248464953696 25.157241599588897 28-29 24.724044476097422 24.763270248354655 24.931515019428044 25.58117025611988 30-31 24.47681852999065 24.77524874606796 25.05089836204591 25.697034361895486 32-33 24.64685087548802 24.99830256800835 25.10021254154481 25.25463401495882 34-35 24.914948413979733 24.881198394759906 24.69690330119778 25.50694989006258 36-37 24.640634197072597 25.01671903846747 24.77542167592974 25.567225088530193 38-39 24.6998364065977 25.17627512594924 25.024588976220986 25.09929949123208 40-41 24.940779308923627 24.831208855652736 24.855878803785615 25.372133031638022 42-43 24.49756112685664 24.79321884234019 25.15776026231259 25.551459768490588 44-45 24.819195395197724 24.940336101142393 25.089635179572596 25.15083332408729 46-47 25.026756272500638 24.768681260214283 24.743793313638257 25.46076915364682 48-49 24.773411914917588 24.77119224096309 24.8879942526336 25.567401591485723 50-51 24.882557902364677 24.964490376169348 25.00268538647529 25.15026633499069 52-53 25.110070690033858 24.7863434623592 24.73791271737671 25.36567313023024 54-55 24.690624043917342 24.755612367716843 24.927496224470165 25.62626736389565 56-57 24.774101947114367 25.083960912251722 25.039756463847024 25.102180676786883 58-59 24.97662852966329 24.908104323921187 24.72232889769174 25.392938248723784 60-61 24.645422924193614 24.85153996267355 24.98366810860725 25.51936900452559 62-63 24.755729953829018 24.993007792261285 25.033535573502043 25.217726680407655 64-65 24.948973095230837 24.8518742744618 24.736123789659445 25.463028840647922 66-67 24.688070871738816 24.845631887978293 24.914883002125727 25.55141423815717 68-69 24.955911655452905 25.03651782574085 24.931422115937206 25.07614840286904 70-71 25.03872443637022 24.875805241672484 24.73793930532405 25.347531016633244 72-73 24.730049510532083 24.80342416058322 24.996602313828767 25.469924015055934 74-75 24.923215908086156 25.040435273449063 24.98617473727644 25.05017408118834 76-77 25.084490078056636 24.943696991602884 24.65483880336104 25.316974126979435 78-79 24.793537860438363 24.852379656722867 24.963191195979505 25.39089128685927 80-81 25.085707626167046 25.10604611478749 24.812079745120325 24.99616651392514 82-83 25.161688914998177 24.967924818577536 24.608468093844323 25.26191817257996 84-85 24.760002539517394 24.950991567275256 24.909878133397324 25.37912775981003 86-87 25.012266671258182 25.2038599057233 24.823497013132805 24.960376409885715 88-89 25.108479291458398 24.999030233899386 24.599876044163942 25.292614430478277 90-91 24.926835586426684 24.844033909331444 24.786913665876682 25.44221683836519 92-93 25.045856897989836 25.104639910050334 24.82537384791573 25.024129344044095 94-95 25.2358228402136 24.97251868561579 24.586954096361175 25.20470437780944 96-97 24.958961885446005 24.993747634387177 24.796979373418942 25.250311106747876 98-99 25.129852003873893 25.157609778913525 24.776368163594427 24.936170053618152 100-101 25.446231346125476 24.968760609909303 24.374066427251563 25.210941616713654 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13133.0 1 7150.5 2 874.5 3 508.0 4 434.0 5 423.0 6 403.5 7 408.0 8 401.5 9 379.0 10 403.5 11 437.5 12 466.5 13 516.5 14 568.5 15 642.0 16 752.0 17 865.0 18 1013.5 19 1232.5 20 1604.5 21 2166.5 22 2896.5 23 4080.0 24 5855.5 25 8490.5 26 12285.0 27 17753.5 28 25362.0 29 35029.0 30 47741.5 31 64775.0 32 86599.5 33 114337.5 34 151107.0 35 197171.5 36 253966.5 37 326138.5 38 410343.0 39 497763.0 40 585191.0 41 669153.5 42 744128.5 43 796570.5 44 821118.0 45 822001.5 46 808278.0 47 781278.0 48 741522.0 49 701039.0 50 663193.0 51 623372.5 52 591898.5 53 575103.0 54 559119.5 55 552060.5 56 555318.0 57 547872.5 58 525992.5 59 495526.5 60 471903.5 61 452701.5 62 426791.0 63 389817.5 64 338365.0 65 298159.0 66 278166.0 67 259593.5 68 229713.0 69 195490.5 70 166426.5 71 138648.0 72 112595.0 73 90798.5 74 70454.5 75 52149.5 76 36891.0 77 25521.0 78 17646.5 79 11839.5 80 7632.5 81 4913.5 82 3157.5 83 2022.0 84 1297.0 85 957.0 86 718.5 87 535.0 88 423.0 89 349.0 90 262.0 91 186.5 92 147.5 93 126.5 94 106.5 95 71.5 96 39.0 97 23.5 98 12.5 99 6.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09472104588004912 2 0.08423288467992911 3 0.08934643911571237 4 0.1071798962270926 5 0.09080526095174662 6 0.09217194667182084 7 0.1000035165284258 8 0.0878313118754803 9 0.114990991391262 10-11 0.08763424296078795 12-13 0.08125381693055389 14-15 0.131245337848549 16-17 0.13114296438637119 18-19 0.12395890667804103 20-21 0.13552710790413727 22-23 0.13380723373954953 24-25 0.10835463170558333 26-27 0.11177390534232329 28-29 0.10138811760438114 30-31 0.08963820348291922 32-33 0.11025621876553676 34-35 0.1290571050944976 36-37 0.11584069112733811 38-39 0.11770644747552929 40-41 0.14727958136215366 42-43 0.12139189211393163 44-45 0.12011734250981748 46-47 0.13753362776282302 48-49 0.14815231512721977 50-51 0.1192292527254247 52-53 0.09070288748956878 54-55 0.09311634186041143 56-57 0.0974928073685142 58-59 0.09414519515529875 60-61 0.09479014796701916 62-63 0.08379011945600995 64-65 0.10558798889022715 66-67 0.10432623596888521 68-69 0.10143674499891561 70-71 0.10180784879931029 72-73 0.099069358686053 74-75 0.13686308158555813 76-77 0.1537060754503677 78-79 0.15238545778827356 80-81 0.12308873224952936 82-83 0.10265754853538639 84-85 0.10643768862630323 86-87 0.11288721674350734 88-89 0.10901749987318488 90-91 0.11371388245059343 92-93 0.10042836639646387 94-95 0.09705260148114948 96-97 0.10083786024517521 98-99 0.0893592357984846 100-101 0.09667381967109147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.9536313E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.69025396292948 #Duplication Level Percentage of deduplicated Percentage of total 1 72.36965457549739 35.960665150659466 2 13.893260295101792 13.807192648733855 3 5.363858550924499 7.995944808500079 4 2.6512926552578566 5.269736214792502 5 1.5224689075664448 3.7825933333820227 6 0.8570860526778362 2.5553294175387866 7 0.6206539459367747 2.1588316537684844 8 0.4807495594061796 1.9110854159567616 9 0.3524838430357965 1.5763510510450292 >10 1.733653707903598 15.007815669945904 >50 0.10131585794359674 3.4702079460238693 >100 0.049247925656360304 4.256668154288127 >500 0.002842153998041556 0.9902762354170487 >1k 0.0014213751214762096 1.2107038520201105 >5k 1.059397222719367E-5 0.04659844792783346 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.02006007991374831 4.606805798002929E-5 5.1186731088921435E-6 2 0.020459336416241897 4.606805798002929E-5 1.0237346217784287E-5 3 0.021984701002691755 4.606805798002929E-5 1.0237346217784287E-5 4 0.02813222740647122 4.606805798002929E-5 1.0237346217784287E-5 5 0.02939653966436758 5.6305404197813576E-5 1.0237346217784287E-5 6 0.03319459511116555 7.678009663338216E-5 2.559336554446072E-5 7 0.03398287076993494 8.701744285116645E-5 3.0712038653352864E-5 8 0.03615318816810521 9.213611596005859E-5 3.5830711762245E-5 9 0.038692050030115714 9.725478906895072E-5 4.606805798002929E-5 10-11 0.044427523248629364 1.1261080839562715E-4 4.606805798002929E-5 12-13 0.05100245885700132 1.4076351049453393E-4 4.862739453447537E-5 14-15 0.05774119200485782 1.6123820293010254E-4 6.142407730670573E-5 16-17 0.06478192686613896 1.7915355881122503E-4 6.654275041559787E-5 18-19 0.07191991651648907 1.8427223192011717E-4 7.422076007893607E-5 20-21 0.07778591589927947 1.996282512467936E-4 7.678009663338216E-5 22-23 0.0830325558358939 2.380182995634847E-4 8.18987697422743E-5 24-25 0.08774941310573801 2.559336554446072E-4 8.701744285116645E-5 26-27 0.09221545539324641 2.7640834788017574E-4 9.725478906895074E-5 28-29 0.09626432582238009 2.8664569409796E-4 1.0237346217784288E-4 30-31 0.09988322771036684 3.327137520779893E-4 1.0749213528673501E-4 32-33 0.10285973612318763 3.531884445135579E-4 1.177294815045193E-4 34-35 0.10582344785323618 3.6598512728578823E-4 1.2028881805896537E-4 36-37 0.10864383673623575 4.094938487113715E-4 1.2284815461341146E-4 38-39 0.11160242979317542 4.1205318526581756E-4 1.279668277223036E-4 40-41 0.11432812322366047 4.197311949291557E-4 1.3820417394008787E-4 42-43 0.11714339343355115 4.4020588736472433E-4 1.7915355881122503E-4 44-45 0.11978718809429395 4.504432335825086E-4 1.9195024158345537E-4 46-47 0.12259222095796685 4.6068057980029294E-4 1.9450957813790145E-4 48-49 0.12516947286829402 5.067486377803223E-4 2.0474692435568575E-4 50-51 0.12780814885592792 5.323420033247829E-4 2.1498427057347003E-4 52-53 0.1304007567855818 5.400200129881212E-4 2.2266228023680825E-4 54-55 0.13311877220640353 5.528166957603515E-4 2.4057763611793075E-4 56-57 0.13583678762722529 5.630540419781357E-4 2.456963092268229E-4 58-59 0.1384780229514136 5.681727150870279E-4 2.456963092268229E-4 60-61 0.14159017620162004 5.860880709681504E-4 2.5337431889016113E-4 62-63 0.14472280414426203 6.116814365126112E-4 2.610523285534993E-4 64-65 0.14803970431882413 6.321561289481796E-4 2.610523285534993E-4 66-67 0.15126190904087172 6.475121482748562E-4 2.6617100166239147E-4 68-69 0.15474004741836395 6.577494944926404E-4 2.9432370376129824E-4 70-71 0.1584715601147463 6.705461772648708E-4 2.9688304031574435E-4 72-73 0.16285570363251245 6.782241869282091E-4 3.0200171342463646E-4 74-75 0.16777218915360334 7.012582159182236E-4 3.0200171342463646E-4 76-77 0.1731135245427323 7.550042835615912E-4 3.0200171342463646E-4 78-79 0.17935062772591737 7.754789759971598E-4 3.096797230879747E-4 80-81 0.18652188875147524 8.138690243138508E-4 3.173577327513129E-4 82-83 0.19542582062439315 8.16428360868297E-4 3.2247640586020507E-4 84-85 0.20740351569920076 8.292250436405272E-4 3.250357424146511E-4 86-87 0.22152593480663418 8.52259072630542E-4 3.3015441552354324E-4 88-89 0.23866581171175955 8.932084575016791E-4 3.3783242518688146E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 1440 0.0 12.861282 1 CTCGTAT 5165 0.0 11.952404 1 CCGATTA 4875 0.0 11.690027 3 CGGGGAT 8300 0.0 11.099561 1 CGGAAAT 6025 0.0 10.955695 1 CTCGACT 9440 0.0 10.916162 1 CTCGGAT 10590 0.0 10.672427 1 GTGCACA 9935 0.0 10.611839 6 CTGGAAT 17290 0.0 10.162228 1 TGCACAC 10505 0.0 10.126406 7 CGCGAAT 2410 0.0 10.049289 1 CTCGAAT 8750 0.0 9.877465 1 CTGGGAT 12365 0.0 9.831687 1 CTCGTGT 6485 0.0 9.81244 1 CTCGGTT 7715 0.0 9.78686 1 CCGGGAT 8665 0.0 9.755142 1 GCACACA 10675 0.0 9.69869 8 GTCCGAT 6910 0.0 9.621265 1 CTCGATT 10615 0.0 9.528879 1 CGAATTA 5325 0.0 9.457821 14-15 >>END_MODULE