FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 mx24-2.3420000000ce23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 mx24-2.3420000000ce23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19536313
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT14400.012.8612821
CTCGTAT51650.011.9524041
CCGATTA48750.011.6900273
CGGGGAT83000.011.0995611
CGGAAAT60250.010.9556951
CTCGACT94400.010.9161621
CTCGGAT105900.010.6724271
GTGCACA99350.010.6118396
CTGGAAT172900.010.1622281
TGCACAC105050.010.1264067
CGCGAAT24100.010.0492891
CTCGAAT87500.09.8774651
CTGGGAT123650.09.8316871
CTCGTGT64850.09.812441
CTCGGTT77150.09.786861
CCGGGAT86650.09.7551421
GCACACA106750.09.698698
GTCCGAT69100.09.6212651
CTCGATT106150.09.5288791
CGAATTA53250.09.45782114-15
Produced by FastQC (version 0.11.2)