##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 mx12-3.3420000000cdb7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17839626 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35948948705539 33.0 33.0 33.0 33.0 33.0 2 32.2932744217844 33.0 33.0 33.0 33.0 33.0 3 32.28368873876616 33.0 33.0 33.0 33.0 33.0 4 36.115342888914824 37.0 37.0 37.0 37.0 37.0 5 36.09509565951663 37.0 37.0 37.0 37.0 37.0 6 36.08876598646182 37.0 37.0 37.0 37.0 37.0 7 36.13823008397149 37.0 37.0 37.0 37.0 37.0 8 36.13495277311307 37.0 37.0 37.0 37.0 37.0 9 36.11833678575997 37.0 37.0 37.0 37.0 37.0 10-11 36.10371201167558 37.0 37.0 37.0 37.0 37.0 12-13 36.09864691109556 37.0 37.0 37.0 37.0 37.0 14-15 38.25985242067294 40.0 37.0 40.0 37.0 40.0 16-17 38.237055418090044 40.0 37.0 40.0 37.0 40.0 18-19 38.20470916823032 40.0 37.0 40.0 37.0 40.0 20-21 38.172949225505064 40.0 37.0 40.0 37.0 40.0 22-23 38.166678382158906 40.0 37.0 40.0 37.0 40.0 24-25 38.104570465770976 40.0 37.0 40.0 37.0 40.0 26-27 38.035017550255816 40.0 37.0 40.0 33.0 40.0 28-29 38.003161641393156 40.0 37.0 40.0 33.0 40.0 30-31 37.93932538159713 40.0 37.0 40.0 33.0 40.0 32-33 37.85752103211132 40.0 37.0 40.0 33.0 40.0 34-35 37.78401183971009 40.0 37.0 40.0 33.0 40.0 36-37 37.70697129525025 40.0 37.0 40.0 33.0 40.0 38-39 37.60207313202642 40.0 37.0 40.0 33.0 40.0 40-41 37.4825230360771 40.0 37.0 40.0 33.0 40.0 42-43 37.36712378387305 40.0 37.0 40.0 33.0 40.0 44-45 37.24619807052009 40.0 37.0 40.0 33.0 40.0 46-47 37.097668219053475 40.0 37.0 40.0 33.0 40.0 48-49 36.937037889695674 40.0 37.0 40.0 33.0 40.0 50-51 35.48517272727578 37.0 35.0 38.5 30.0 38.5 52-53 35.80282742474534 37.0 35.0 38.5 33.0 40.0 54-55 36.47247784230454 37.0 37.0 40.0 33.0 40.0 56-57 36.418445039150484 37.0 37.0 40.0 33.0 40.0 58-59 36.262944918239874 37.0 37.0 40.0 33.0 40.0 60-61 36.08421964675717 37.0 37.0 40.0 33.0 40.0 62-63 35.91455283311433 37.0 37.0 40.0 33.0 40.0 64-65 35.71886574303744 37.0 37.0 38.5 33.0 40.0 66-67 35.501587168923834 37.0 37.0 37.0 33.0 40.0 68-69 35.29757776312127 37.0 35.0 37.0 33.0 40.0 70-71 35.10210620446863 37.0 33.0 37.0 30.0 40.0 72-73 34.916991841645114 37.0 33.0 37.0 27.0 40.0 74-75 34.71382925852818 37.0 33.0 37.0 27.0 37.0 76-77 34.54437915346432 37.0 33.0 37.0 27.0 37.0 78-79 34.37838200756002 37.0 33.0 37.0 27.0 37.0 80-81 34.23184606000149 37.0 33.0 37.0 27.0 37.0 82-83 34.13176447757369 37.0 33.0 37.0 27.0 37.0 84-85 34.037734871796076 37.0 33.0 37.0 27.0 37.0 86-87 33.933090357387535 37.0 33.0 37.0 27.0 37.0 88-89 33.84645950537304 37.0 33.0 37.0 27.0 37.0 90-91 33.76121525753959 37.0 33.0 37.0 27.0 37.0 92-93 33.686760669758435 37.0 33.0 37.0 27.0 37.0 94-95 33.604612927423474 37.0 33.0 37.0 27.0 37.0 96-97 33.515291408014946 37.0 33.0 37.0 27.0 37.0 98-99 33.44828792935457 37.0 33.0 37.0 27.0 37.0 100-101 31.630476137784502 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15536110121600188 2306 2 0.25508199650665375 2306 3 0.2989623652222022 2306 4 0.4058138742260695 2306 5 0.38416506978906995 2306 6 0.44215368913936004 2306 7 0.41316429222656126 2306 8 0.43462881148700916 2306 9 0.45119707506978557 2306 10-11 0.4694356078619606 2306 12-13 0.4409640631793863 2306 14-15 0.7665506918079004 2306 16-17 0.758558538937379 2306 18-19 0.7857558586590372 2306 20-21 0.7912502398698606 2306 22-23 0.7844323825116462 2306 24-25 0.7947088794943369 2306 26-27 0.8388272838857489 2306 28-29 0.8365658343071445 2306 30-31 0.8107024986025095 2306 32-33 0.8749325101097796 2306 34-35 0.8738828410162682 2306 36-37 0.9165392508723684 2306 38-39 0.8082620030194008 2306 40-41 0.892033782481775 2306 42-43 0.8723288056653686 2306 44-45 0.8696407323720692 2306 46-47 0.8830149312173248 2306 48-49 0.8732320336365191 2306 50-51 0.7596189611278419 2306 52-53 0.8415821267635906 2306 54-55 0.9043160727906425 2306 56-57 0.9339697515697623 2306 58-59 0.9515637866433835 2306 60-61 0.9082223261125293 2306 62-63 0.9352603016463732 2306 64-65 0.9269256168831816 2306 66-67 0.8468537909608074 2306 68-69 0.8815877353696777 2306 70-71 0.9074774222182143 2306 72-73 0.9021738639293844 2306 74-75 0.9284628366311836 2306 76-77 0.8690440572528217 2306 78-79 0.8064144255472243 2306 80-81 0.9134187642194433 2306 82-83 0.9491286812322741 2306 84-85 0.9572244263634744 2306 86-87 0.9772148034534638 2306 88-89 0.929968939296451 2306 90-91 0.9765277151768572 2306 92-93 0.9890791621870036 2306 94-95 0.9249853901067624 2306 96-97 0.9071822887267231 2306 98-99 0.9080652013480872 2306 100-101 0.7775466725710096 2307 1 0.18234711850453778 2307 2 0.2577532651985095 2307 3 0.2681658341670854 2307 4 0.38412679465437094 2307 5 0.39181691538429675 2307 6 0.4000563377428108 2307 7 0.3892319338666397 2307 8 0.4070471055118219 2307 9 0.36814153930904325 2307 10-11 0.37569462012901766 2307 12-13 0.4001088383706417 2307 14-15 0.6543739300522802 2307 16-17 0.6382652023209516 2307 18-19 0.627317894941072 2307 20-21 0.6489998491334248 2307 22-23 0.6972475082523744 2307 24-25 0.7083881769937506 2307 26-27 0.7044624149226024 2307 28-29 0.7119168276676291 2307 30-31 0.7272072027967198 2307 32-33 0.7332737238429914 2307 34-35 0.7396455496384604 2307 36-37 0.6962653832241372 2307 38-39 0.6596661915114126 2307 40-41 0.7389032630982229 2307 42-43 0.720140626138587 2307 44-45 0.7660025812345026 2307 46-47 0.7123883762557597 2307 48-49 0.713601504567059 2307 50-51 0.6173352593093995 2307 52-53 0.6745503981552474 2307 54-55 0.8347765118073696 2307 56-57 0.7616611408835539 2307 58-59 0.8026860774482643 2307 60-61 0.8074254546192918 2307 62-63 0.817168795900308 2307 64-65 0.7858798447076794 2307 66-67 0.7896245673076834 2307 68-69 0.8098478219852581 2307 70-71 0.7625708509801328 2307 72-73 0.7154590839145882 2307 74-75 0.7319175283828372 2307 76-77 0.7153892969661015 2307 78-79 0.7811141406457693 2307 80-81 0.7864998607618219 2307 82-83 0.6965501962388387 2307 84-85 0.7573177988481774 2307 86-87 0.7829109672602996 2307 88-89 0.7782397399144969 2307 90-91 0.7067981331792126 2307 92-93 0.7071288918560654 2307 94-95 0.7132918120341998 2307 96-97 0.7436011208276128 2307 98-99 0.7522992867456111 2307 100-101 0.6940634126318947 2201 1 0.20818124328403087 2201 2 0.25048872183187854 2201 3 0.27194012206744134 2201 4 0.35349411209746506 2201 5 0.34379619781923054 2201 6 0.41132171758217595 2201 7 0.3669701013170936 2201 8 0.33633264753085257 2201 9 0.3733822275783467 2201 10-11 0.34842703756574167 2201 12-13 0.38594956034749117 2201 14-15 0.6312250425413239 2201 16-17 0.6541347085452003 2201 18-19 0.6001191077800314 2201 20-21 0.6598345611519889 2201 22-23 0.6687340441710177 2201 24-25 0.6769978847306746 2201 26-27 0.662261091635898 2201 28-29 0.6679017788778125 2201 30-31 0.6929817207565279 2201 32-33 0.737287921806562 2201 34-35 0.7253788449189287 2201 36-37 0.7340658235922106 2201 38-39 0.7133777842586255 2201 40-41 0.7386872781639084 2201 42-43 0.7359325248868842 2201 44-45 0.7637356849589381 2201 46-47 0.7439086717945713 2201 48-49 0.7401971413210688 2201 50-51 0.6710485183678259 2201 52-53 0.7552783479868452 2201 54-55 0.8387990258264395 2201 56-57 0.8393138668702704 2201 58-59 0.7551523606484167 2201 60-61 0.81630347550783 2201 62-63 0.8110759134502317 2201 64-65 0.8469277600833749 2201 66-67 0.8453857693483258 2201 68-69 0.7974106300987884 2201 70-71 0.8495071276277244 2201 72-73 0.8667029565588678 2201 74-75 0.8971577796910921 2201 76-77 0.9002096505591979 2201 78-79 0.863696167837837 2201 80-81 0.9010150721603694 2201 82-83 0.8649261739895664 2201 84-85 0.8856968667924221 2201 86-87 0.8894359838850434 2201 88-89 0.8908239252508352 2201 90-91 0.8606792223279598 2201 92-93 0.9263842913127931 2201 94-95 0.8821453739542235 2201 96-97 0.8990078263956534 2201 98-99 0.8723367038801513 2201 100-101 0.7797594809698118 1101 1 0.2368108581895143 1101 2 0.26531120394238883 1101 3 0.24729376754797272 1101 4 0.3511953000810806 1101 5 0.3175643118735678 1101 6 0.36514008635889894 1101 7 0.3238215475647266 1101 8 0.3540918569576945 1101 9 0.37044399159089636 1101 10-11 0.38477375782259315 1101 12-13 0.35331148478706353 1101 14-15 0.5974194432661548 1101 16-17 0.6006387018269805 1101 18-19 0.5866268674148927 1101 20-21 0.5931751531731067 1101 22-23 0.5924508879829773 1101 24-25 0.6254633416096667 1101 26-27 0.6340713669996347 1101 28-29 0.6435654357933842 1101 30-31 0.6676081969980387 1101 32-33 0.7062132561847392 1101 34-35 0.7179346793351655 1101 36-37 0.6856415152059654 1101 38-39 0.6228252241092704 1101 40-41 0.7039371517836912 1101 42-43 0.7183857274789105 1101 44-45 0.667410129924292 1101 46-47 0.7259066106904157 1101 48-49 0.7118566041855487 1101 50-51 0.6792693560350074 1101 52-53 0.7104009663583426 1101 54-55 0.7407759358948667 1101 56-57 0.7251271476434127 1101 58-59 0.7464196021993317 1101 60-61 0.7633654893726955 1101 62-63 0.7596777105082637 1101 64-65 0.7750844284383902 1101 66-67 0.7524278686179002 1101 68-69 0.7211175583234279 1101 70-71 0.7332073540758444 1101 72-73 0.7116751482686183 1101 74-75 0.7177372750128868 1101 76-77 0.7777788594472881 1101 78-79 0.7759570813530772 1101 80-81 0.7858495052671586 1101 82-83 0.8073246840450921 1101 84-85 0.8143463415441445 1101 86-87 0.8021465735520863 1101 88-89 0.8315690000267253 1101 90-91 0.7392236812820485 1101 92-93 0.7560892330612958 1101 94-95 0.7597287820998986 1101 96-97 0.7481876574807345 1101 98-99 0.7681460025807283 1101 100-101 0.7828033751347725 2304 1 0.14322906334544427 2304 2 0.2636798430514986 2304 3 0.26310056440554064 2304 4 0.34088730697177994 2304 5 0.3639573201238946 2304 6 0.3531138153512643 2304 7 0.32883722669404136 2304 8 0.35394747572438234 2304 9 0.32431135582987736 2304 10-11 0.3869195014254032 2304 12-13 0.3623130512149757 2304 14-15 0.6600911189148349 2304 16-17 0.6357599063563555 2304 18-19 0.6181092339195402 2304 20-21 0.6778923231926157 2304 22-23 0.6838894724837559 2304 24-25 0.6949078103190516 2304 26-27 0.6965427986750399 2304 28-29 0.7317438972872239 2304 30-31 0.7283201561900796 2304 32-33 0.7465507841881802 2304 34-35 0.7832754350606379 2304 36-37 0.7666066294271445 2304 38-39 0.7275629187720725 2304 40-41 0.7608706524985109 2304 42-43 0.7921109097614973 2304 44-45 0.8210590675014586 2304 46-47 0.7995946676180594 2304 48-49 0.8098553408169948 2304 50-51 0.7647844227269971 2304 52-53 0.8900813802382075 2304 54-55 0.8922974013674363 2304 56-57 0.8515043236041393 2304 58-59 0.8644429618220713 2304 60-61 0.8838201302546267 2304 62-63 0.890409967223782 2304 64-65 0.9019808498045876 2304 66-67 0.8935938787627009 2304 68-69 0.9020973086698234 2304 70-71 0.9311880710791343 2304 72-73 0.9288557958198354 2304 74-75 0.9001387226280215 2304 76-77 0.934272188850862 2304 78-79 0.918909840197891 2304 80-81 0.9674982545095858 2304 82-83 0.9537449510413225 2304 84-85 0.975650490912578 2304 86-87 1.0382972874682963 2304 88-89 1.016901064924987 2304 90-91 0.9682532134390982 2304 92-93 0.9751761219990627 2304 94-95 1.0278279021969539 2304 96-97 1.0264720022339446 2304 98-99 0.9963019896949987 2304 100-101 1.1236179843317338 2202 1 0.21245939997930208 2202 2 0.24431526595913056 2202 3 0.2515916411250032 2202 4 0.2503210330766805 2202 5 0.27007748402551357 2202 6 0.276990548254183 2202 7 0.2763612740758816 2202 8 0.27694104599156333 2202 9 0.225480543845336 2202 10-11 0.252818624160561 2202 12-13 0.2676475299922245 2202 14-15 0.4329702182649271 2202 16-17 0.42368428203710096 2202 18-19 0.42140269892589544 2202 20-21 0.43611002739631033 2202 22-23 0.43147863097089356 2202 24-25 0.42041280935902137 2202 26-27 0.40625072179609845 2202 28-29 0.445456446784803 2202 30-31 0.43082095700991374 2202 32-33 0.48038586731109945 2202 34-35 0.46865946488133403 2202 36-37 0.4283104273058811 2202 38-39 0.516852417478809 2202 40-41 0.46509946672576064 2202 42-43 0.45419031403375243 2202 44-45 0.4854696185328251 2202 46-47 0.47748960374995875 2202 48-49 0.43693837044843775 2202 50-51 0.49118482978235534 2202 52-53 0.5601741329572576 2202 54-55 0.5811896148012821 2202 56-57 0.5565567982282644 2202 58-59 0.5663307519662268 2202 60-61 0.5907823598561706 2202 62-63 0.5865350626385535 2202 64-65 0.6295317387250208 2202 66-67 0.5607711677327458 2202 68-69 0.5610508318094247 2202 70-71 0.49833322300197125 2202 72-73 0.5728857785619184 2202 74-75 0.5555184029708897 2202 76-77 0.571574236524981 2202 78-79 0.572159110434626 2202 80-81 0.5525074196296629 2202 82-83 0.5862659479504586 2202 84-85 0.5607967649661347 2202 86-87 0.5213879075074743 2202 88-89 0.5803451752071496 2202 90-91 0.5449384752504329 2202 92-93 0.5771351921079813 2202 94-95 0.527351911094172 2202 96-97 0.5132959796821908 2202 98-99 0.5342265649477227 2202 100-101 0.5773322900627953 1102 1 0.15636578155958603 1102 2 0.16404816360306285 1102 3 0.16956851427516284 1102 4 0.20777937667061508 1102 5 0.18148327253364016 1102 6 0.23526238510019226 1102 7 0.21134833709496803 1102 8 0.2222426270542428 1102 9 0.23288610600828008 1102 10-11 0.19772906738919716 1102 12-13 0.2548155389943574 1102 14-15 0.376542074851983 1102 16-17 0.37056929275664174 1102 18-19 0.34013020100684344 1102 20-21 0.36120116495605004 1102 22-23 0.35631396440928853 1102 24-25 0.4380176353603602 1102 26-27 0.40968280448234395 1102 28-29 0.4251467206580415 1102 30-31 0.3486362229747897 1102 32-33 0.44655514235969207 1102 34-35 0.3629680515839695 1102 36-37 0.44957284941344966 1102 38-39 0.440971401158869 1102 40-41 0.4300419871722596 1102 42-43 0.4436995489259843 1102 44-45 0.444740218974907 1102 46-47 0.3744195295648396 1102 48-49 0.40176515886957276 1102 50-51 0.4487057943632351 1102 52-53 0.4236315927608558 1102 54-55 0.535504341935443 1102 56-57 0.5396233228289731 1102 58-59 0.5299983110652917 1102 60-61 0.5053983477365307 1102 62-63 0.49539724850829003 1102 64-65 0.5106187849053612 1102 66-67 0.5359371195421119 1102 68-69 0.5153170071058923 1102 70-71 0.5652631435077353 1102 72-73 0.5511233862296265 1102 74-75 0.6160304023182235 1102 76-77 0.563088329609478 1102 78-79 0.5342728426056311 1102 80-81 0.5451895441030601 1102 82-83 0.5997708712236047 1102 84-85 0.5670211534735685 1102 86-87 0.5036846085220361 1102 88-89 0.5280068715412867 1102 90-91 0.5111934747757161 1102 92-93 0.4893688770491238 1102 94-95 0.5514145540254489 1102 96-97 0.5393727894503897 1102 98-99 0.6062685064089166 1102 100-101 0.6665769165547566 2305 1 0.20052151430950005 2305 2 0.20657313386084297 2305 3 0.25005192249867036 2305 4 0.22551625638138262 2305 5 0.26848646495647444 2305 6 0.29519992851353294 2305 7 0.2666176053154601 2305 8 0.281648172003365 2305 9 0.21420335194503082 2305 10-11 0.2705370348054501 2305 12-13 0.268588192102051 2305 14-15 0.4443525766915073 2305 16-17 0.3959054903545365 2305 18-19 0.3780740467871766 2305 20-21 0.41143573952509627 2305 22-23 0.3691180776248615 2305 24-25 0.36726935173599173 2305 26-27 0.4291191697366088 2305 28-29 0.43815462464974786 2305 30-31 0.46614704326028544 2305 32-33 0.4405561615168878 2305 34-35 0.46232357765601506 2305 36-37 0.44626518008441707 2305 38-39 0.48416112064614936 2305 40-41 0.45117458914385367 2305 42-43 0.42481010047468004 2305 44-45 0.460122474846969 2305 46-47 0.4566771230373803 2305 48-49 0.42146113371541105 2305 50-51 0.49584455949786843 2305 52-53 0.4912596541611123 2305 54-55 0.5626885434502924 2305 56-57 0.5745523743952035 2305 58-59 0.5013986654491092 2305 60-61 0.4901180465531496 2305 62-63 0.5211165005032967 2305 64-65 0.5726675697537331 2305 66-67 0.5473105102782014 2305 68-69 0.5532464534278034 2305 70-71 0.5599347508864767 2305 72-73 0.5514635662869409 2305 74-75 0.5508544803712425 2305 76-77 0.5449417356424178 2305 78-79 0.5804108549105749 2305 80-81 0.5933287126173781 2305 82-83 0.5817502550452147 2305 84-85 0.6007910306350723 2305 86-87 0.46124345120256294 2305 88-89 0.538366910186312 2305 90-91 0.47168505021785734 2305 92-93 0.6365701352826463 2305 94-95 0.6857028400696237 2305 96-97 0.6780799746865327 2305 98-99 0.6291990308158901 2305 100-101 0.7333672806193228 2203 1 0.15411377097967005 2203 2 0.14543356363343918 2203 3 0.1349893330634231 2203 4 0.1917618987029357 2203 5 0.19590689114716753 2203 6 0.18608520081449598 2203 7 0.18342722145759183 2203 8 0.13050354204337822 2203 9 0.20015827047890866 2203 10-11 0.20217862914618223 2203 12-13 0.19557485393696794 2203 14-15 0.2619418212844593 2203 16-17 0.28502823859923865 2203 18-19 0.23415340236846305 2203 20-21 0.27761365994415854 2203 22-23 0.2657183163833423 2203 24-25 0.2745875328155378 2203 26-27 0.2543534740756286 2203 28-29 0.2941329070196943 2203 30-31 0.33147944603931734 2203 32-33 0.24628675342859196 2203 34-35 0.316758282264054 2203 36-37 0.3327401852501666 2203 38-39 0.3910337728316833 2203 40-41 0.32562326317376744 2203 42-43 0.28858150360836277 2203 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882.0 6 755.0 7 1163.0 8 2218.0 9 5745.0 10 16250.0 11 20733.0 12 18670.0 13 20128.0 14 22774.0 15 26339.0 16 30704.0 17 36272.0 18 41480.0 19 47240.0 20 52524.0 21 59832.0 22 66829.0 23 74765.0 24 86443.0 25 98679.0 26 117112.0 27 143142.0 28 163267.0 29 191631.0 30 238259.0 31 303917.0 32 400059.0 33 540041.0 34 742182.0 35 1144354.0 36 2391668.0 37 6100885.0 38 4611717.0 39 4461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.748763496279114 12.058044149028065 10.989754535165556 55.20343781952727 2 22.099070255230842 18.853157507355615 40.15386100837746 18.893911229036085 3 24.67858643507815 23.686571579850803 23.43535997225193 28.19948201281912 4 28.273066794642887 28.725631912992927 16.87983098968085 26.12147030268333 5 29.002953497010033 29.48718898228912 20.997366315546145 20.512491205154706 6 21.14430659122522 32.229790019391515 23.405159733847896 23.220743655535365 7 19.123027677811656 14.315368983714164 41.3588054529085 25.20279788556568 8 21.19467722224423 19.263684056207193 27.74954434075311 31.792094380795465 9 22.41457706385143 19.173567095424307 30.687397304525454 27.72445853619881 10-11 25.50943995814973 28.119658925276532 20.80659271840621 25.564308398167523 12-13 23.4565400528573 22.166109683495772 27.605921615278124 26.771428648368804 14-15 24.386283133559747 23.858899385004758 25.695583389487354 26.05923409194815 16-17 25.56301082325552 24.118332823435644 24.429224942509165 25.889431410799666 18-19 25.252806082337305 24.29524408658455 24.599740365454466 25.852209465623684 20-21 25.074786451925945 24.618691819194055 24.910961292467963 25.39556043641204 22-23 25.294061696494317 24.501885240980872 24.589827509494548 25.614225553030263 24-25 25.110908413618265 24.666082255899635 24.533944478620185 25.689064851861914 26-27 24.84233779398592 24.62755617526757 24.92090267879067 25.609203351955838 28-29 25.146106751435017 24.450686844330686 24.55787243105513 25.845333973179162 30-31 25.223753903724962 24.131734870609343 24.638867744286735 26.005643481378964 32-33 25.283118886783367 24.433511093966136 24.630325993512308 25.653044025738186 34-35 25.315424118358138 24.644778551241338 24.199586172874238 25.840211157526284 36-37 25.103274944131282 24.490947793128477 24.487889145868262 25.91788811687198 38-39 25.102033508847637 24.35079827775642 24.841778678893007 25.70538953450293 40-41 25.62000456693229 24.161286533177698 24.566059328296046 25.65264957159396 42-43 25.26290739965643 24.159850392994805 24.877532977341325 25.69970923000744 44-45 25.32490191773912 24.310448178692383 24.8236214260312 25.541028477537296 46-47 25.460508976884665 24.166648656856417 24.50869003750625 25.864152328752667 48-49 25.213372679494217 24.19779911810226 24.498346554014667 26.090481648388863 50-51 25.280623459645856 24.297022119026675 24.869148646654736 25.553205774672733 52-53 25.4932871982802 24.335886115916846 24.611605150301283 25.55922153550167 54-55 25.175565827601304 24.239534565924583 24.63052434835714 25.95437525811697 56-57 24.917461360065758 24.421258791201915 25.062504022494437 25.59877582623789 58-59 25.462805324033233 24.350531220255863 24.513607673481037 25.67305578222987 60-61 25.185287990087264 24.38294872158504 24.547823408098278 25.883939880229423 62-63 25.07134176146385 24.592990303231765 24.77609210492174 25.55957583038265 64-65 25.228332202363575 24.63459482406324 24.44698805474277 25.690084918830415 66-67 25.106479949884992 24.566108390743224 24.44692054146187 25.88049111790992 68-69 25.196607846200394 24.705126107344768 24.585845536907904 25.512420509546935 70-71 25.39892849936266 24.514288994527128 24.274380859243045 25.812401646867166 72-73 25.262576835487536 24.53852414701351 24.561535865258282 25.637363152240667 74-75 25.447560832092147 24.563061079796423 24.644946211081653 25.344431877029777 76-77 25.634954984715876 24.555580776298974 24.322976964977407 25.486487274007747 78-79 25.30877796608133 24.538016264172033 24.390101970248036 25.763103799498598 80-81 25.412651921871838 24.652675640612255 24.35698556473866 25.577686872777246 82-83 25.560060652001436 24.5825531419772 24.18871579069521 25.66867041532615 84-85 25.23929001603142 24.58388907569514 24.307562039253856 25.86925886901959 86-87 25.341762850903038 25.016452268302913 24.321058597573938 25.320726283220107 88-89 25.444830181767188 24.68825115871313 24.24580740044377 25.621111259075914 90-91 25.50448920761485 24.445607012440043 24.222628915245398 25.82727486469971 92-93 25.432467843530343 24.758721791746623 24.5198010311382 25.289009333584833 94-95 25.567747628742232 24.75114986524142 24.20706277135549 25.47403973466086 96-97 25.207912269003163 24.807952300070728 24.35722122105586 25.62691420987025 98-99 25.410324631002183 24.774276322099634 24.478509706562093 25.33688934033609 100-101 25.781446760250613 24.78123889796122 23.99239463613733 25.444919705650836 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12400.0 1 6750.0 2 825.5 3 461.0 4 376.0 5 380.0 6 374.0 7 395.0 8 395.0 9 387.0 10 420.0 11 428.5 12 467.5 13 554.0 14 613.5 15 662.5 16 795.0 17 975.0 18 1140.0 19 1383.0 20 1844.5 21 2600.0 22 3803.0 23 5646.5 24 7995.0 25 11424.5 26 16284.0 27 22403.5 28 30875.5 29 41852.0 30 54188.5 31 68715.5 32 86444.5 33 108060.0 34 135566.5 35 175838.0 36 218853.0 37 269065.0 38 326826.0 39 382124.5 40 443820.0 41 504135.0 42 561856.5 43 606517.0 44 639717.0 45 655397.0 46 651147.5 47 651566.5 48 632928.5 49 613138.0 50 624393.5 51 609544.5 52 588300.0 53 593741.0 54 604858.5 55 621313.5 56 635940.5 57 628558.0 58 577653.0 59 505408.0 60 458883.5 61 428554.0 62 390542.0 63 350416.5 64 302577.0 65 270445.5 66 258525.5 67 247294.0 68 222850.5 69 187610.0 70 160739.0 71 135201.5 72 109155.5 73 88514.5 74 76590.5 75 63526.5 76 43607.5 77 28873.5 78 20843.0 79 18118.0 80 13823.5 81 8080.5 82 4168.0 83 1827.0 84 1138.0 85 754.5 86 557.0 87 472.5 88 375.5 89 300.0 90 249.0 91 206.0 92 154.5 93 110.0 94 79.5 95 48.0 96 32.5 97 23.0 98 13.0 99 11.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09753007153849526 2 0.08681235806176654 3 0.0919582058502796 4 0.1101424435691645 5 0.09353335097944318 6 0.09464884521682237 7 0.10264228633492653 8 0.08968797888475914 9 0.11865719606453634 10-11 0.09015323527522381 12-13 0.0842506451648706 14-15 0.13476179377303088 16-17 0.13431055112926696 18-19 0.12701779734619997 20-21 0.139588128136767 22-23 0.1375000798783562 24-25 0.11193059764817939 26-27 0.11545365356874634 28-29 0.10463504111577226 30-31 0.09296719561273313 32-33 0.11404387065065152 34-35 0.13303249743015913 36-37 0.11934947515155306 38-39 0.12166734885585605 40-41 0.1501909288905496 42-43 0.12488210234900665 44-45 0.1236376816419806 46-47 0.14160050216299377 48-49 0.15207717919646968 50-51 0.1226987606130308 52-53 0.09300082860481493 54-55 0.09550368376556773 56-57 0.10074762778098599 58-59 0.09703398490528894 60-61 0.09698914091584655 62-63 0.08626021644175724 64-65 0.10859812868274257 66-67 0.10660257115255667 68-69 0.10436317442977786 70-71 0.1044752844033838 72-73 0.10241806638771464 74-75 0.140342067709267 76-77 0.15700441253645114 78-79 0.1567297431011166 80-81 0.12607327081856987 82-83 0.10619336974889496 84-85 0.1099686731100753 86-87 0.1157787724922036 88-89 0.11251917500961063 90-91 0.11679336775333743 92-93 0.10339622590742653 94-95 0.09952562906868116 96-97 0.10351954687839307 98-99 0.09221886153891343 100-101 0.09938829435101387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7839626E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.32446704927914 #Duplication Level Percentage of deduplicated Percentage of total 1 74.92699453748668 33.210991004783466 2 12.803694794048088 11.350338960156227 3 4.482111751353394 5.960016439021511 4 2.3005675423290715 4.078857208984242 5 1.2472256746780204 2.7641306660140437 6 0.9533423500889782 2.5353834949920757 7 0.6266282167500251 1.9442473221839527 8 0.42239140333622865 1.4977819071260356 9 0.3482891593466427 1.3893958230373256 >10 1.647071652774659 13.320358050986169 >50 0.12445905475286104 3.85039649372807 >100 0.09978585080263437 8.979245848834559 >500 0.01070297599961619 3.241525652836756 >1k 0.006440694024378447 4.860321871182903 >5k 2.561588497555009E-4 0.7255404826248871 >10k+ 3.818337884825994E-5 0.29146877350767675 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 27417 0.15368595731771506 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.1632713600610237 1.6816496040892338E-5 5.605498680297446E-6 2 0.1639215979079382 1.6816496040892338E-5 5.605498680297446E-6 3 0.16588352244604232 1.6816496040892338E-5 5.605498680297446E-6 4 0.17212244247721337 1.6816496040892338E-5 1.6816496040892338E-5 5 0.17319869822383047 2.2421994721189784E-5 1.6816496040892338E-5 6 0.17603508055606099 3.923849076208212E-5 2.2421994721189784E-5 7 0.17670213489901637 3.923849076208212E-5 2.2421994721189784E-5 8 0.1784006009991465 3.923849076208212E-5 2.2421994721189784E-5 9 0.18122016683533612 4.484398944237957E-5 2.802749340148723E-5 10-11 0.18570456577957406 4.764673878252829E-5 2.802749340148723E-5 12-13 0.19021979496655367 5.325223746282574E-5 2.802749340148723E-5 14-15 0.19556183520887715 7.567423218401552E-5 2.802749340148723E-5 16-17 0.20038256407393296 8.68852295446104E-5 2.802749340148723E-5 18-19 0.20474924754588464 8.968797888475914E-5 2.802749340148723E-5 20-21 0.20854417015244603 1.0370172558550275E-4 2.802749340148723E-5 22-23 0.212086845318394 1.3172921898698997E-4 2.802749340148723E-5 24-25 0.21505215412027134 1.345319683271387E-4 3.3632992081784676E-5 26-27 0.21810434815169333 1.4013746700743614E-4 3.6435741421933396E-5 28-29 0.22051471258422123 1.4574296568773358E-4 3.923849076208212E-5 30-31 0.22289704952334763 1.597567123884772E-4 4.484398944237957E-5 32-33 0.22520371223029 1.6816496040892337E-4 4.764673878252829E-5 34-35 0.22732819623012276 1.9338970447026188E-4 6.166048548327191E-5 36-37 0.2290406760769536 2.2421994721189784E-4 6.726598416356935E-5 38-39 0.2309017016388124 2.2421994721189784E-4 6.726598416356935E-5 40-41 0.23295891965448154 2.2982544589219528E-4 7.006873350371807E-5 42-43 0.23499932117410982 2.4944469127323633E-4 8.68852295446104E-5 44-45 0.23712660792328272 2.8868318203531843E-4 9.529347756505658E-5 46-47 0.2390997434587474 3.054996780762108E-4 9.529347756505658E-5 48-49 0.2408654755430411 3.083024274163595E-4 9.529347756505658E-5 50-51 0.24274892309962104 3.195134247769544E-4 9.529347756505658E-5 52-53 0.24489302634483479 3.2511892345725186E-4 1.0089897624535403E-4 54-55 0.2469642581072047 3.419354194981442E-4 1.0089897624535403E-4 56-57 0.2492933428088683 3.559491661988878E-4 1.0650447492565147E-4 58-59 0.2516168220118516 3.615546648791853E-4 1.0650447492565147E-4 60-61 0.2541280854206248 3.7837116092007757E-4 1.0930722426580019E-4 62-63 0.25676547254970483 4.092014036617135E-4 1.1210997360594892E-4 64-65 0.2597672170930041 4.14806902342011E-4 1.1771547228624636E-4 66-67 0.2626904846547792 4.2041240102230846E-4 1.261237203066925E-4 68-69 0.26593046289199107 4.288206490427546E-4 1.2892646964684124E-4 70-71 0.2698963532083016 4.428343957434982E-4 1.2892646964684124E-4 72-73 0.2743078806696957 4.512426437639444E-4 1.345319683271387E-4 74-75 0.2792967744951603 4.7086188914498544E-4 1.345319683271387E-4 76-77 0.2853787405632831 4.820728865055803E-4 1.345319683271387E-4 78-79 0.2924276551537571 4.820728865055803E-4 1.4013746700743614E-4 80-81 0.3013347925567498 4.820728865055803E-4 1.4294021634758485E-4 82-83 0.3133641927246681 4.904811345260265E-4 1.6255946172862593E-4 84-85 0.3284682089187296 5.129031292472163E-4 1.6536221106877466E-4 86-87 0.34704483154523535 5.297196252881086E-4 1.7096770974907207E-4 88-89 0.3701843300975032 5.521416200092984E-4 1.7657320842936954E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 2250 0.0 26.170374 1 ACATGCG 4175 0.0 22.181223 4 CATGCGT 4595 0.0 19.533606 5 GCGTATT 3050 0.0 18.994627 2 TAAGTCG 2900 0.0 18.67405 9 GCCGTAT 7505 0.0 18.452564 48-49 GAGTGTA 7455 0.0 17.839485 28-29 CCGTATC 8325 0.0 17.548086 48-49 ATCGGAA 17765 0.0 16.279915 3 TCGTGTA 8915 0.0 15.881867 14-15 GTCGTGT 8830 0.0 15.362153 14-15 GTAGGGA 9225 0.0 15.347069 18-19 TGTAGGG 8720 0.0 15.309654 18-19 TCGGAAG 18285 0.0 15.219836 4 AGAGCGT 18510 0.0 15.218783 9 CGTGTAG 8930 0.0 15.189313 16-17 AAGAGCG 18775 0.0 14.620634 8 AGATCGG 18370 0.0 14.553586 1 AAAGGGC 10830 0.0 14.294202 2 CGCCGTA 10305 0.0 14.29268 46-47 >>END_MODULE