FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 mx12-3.3420000000cdb7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 mx12-3.3420000000cdb7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17839626
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC274170.15368595731771506Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT22500.026.1703741
ACATGCG41750.022.1812234
CATGCGT45950.019.5336065
GCGTATT30500.018.9946272
TAAGTCG29000.018.674059
GCCGTAT75050.018.45256448-49
GAGTGTA74550.017.83948528-29
CCGTATC83250.017.54808648-49
ATCGGAA177650.016.2799153
TCGTGTA89150.015.88186714-15
GTCGTGT88300.015.36215314-15
GTAGGGA92250.015.34706918-19
TGTAGGG87200.015.30965418-19
TCGGAAG182850.015.2198364
AGAGCGT185100.015.2187839
CGTGTAG89300.015.18931316-17
AAGAGCG187750.014.6206348
AGATCGG183700.014.5535861
AAAGGGC108300.014.2942022
CGCCGTA103050.014.2926846-47
Produced by FastQC (version 0.11.2)