##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 mx0-3.3420000000cbd3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20943685 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37668194493949 33.0 33.0 33.0 33.0 33.0 2 32.32445149934217 33.0 33.0 33.0 33.0 33.0 3 32.30437122216076 33.0 33.0 33.0 33.0 33.0 4 36.13983513407502 37.0 37.0 37.0 37.0 37.0 5 36.13451147684851 37.0 37.0 37.0 37.0 37.0 6 36.148702293794045 37.0 37.0 37.0 37.0 37.0 7 36.183743787208414 37.0 37.0 37.0 37.0 37.0 8 36.16747993488252 37.0 37.0 37.0 37.0 37.0 9 36.13975405951722 37.0 37.0 37.0 37.0 37.0 10-11 36.12234654503255 37.0 37.0 37.0 37.0 37.0 12-13 36.1207238124523 37.0 37.0 37.0 37.0 37.0 14-15 38.32200009215188 40.0 37.0 40.0 37.0 40.0 16-17 38.298890548630766 40.0 37.0 40.0 37.0 40.0 18-19 38.27741758434583 40.0 37.0 40.0 37.0 40.0 20-21 38.242311035522164 40.0 37.0 40.0 37.0 40.0 22-23 38.26024553463252 40.0 37.0 40.0 37.0 40.0 24-25 38.20324429535681 40.0 37.0 40.0 37.0 40.0 26-27 38.13456485809446 40.0 37.0 40.0 37.0 40.0 28-29 38.0776829626687 40.0 37.0 40.0 37.0 40.0 30-31 38.03063696765875 40.0 37.0 40.0 37.0 40.0 32-33 37.94249414561 40.0 37.0 40.0 33.0 40.0 34-35 37.87047673320144 40.0 37.0 40.0 33.0 40.0 36-37 37.80725252027043 40.0 37.0 40.0 33.0 40.0 38-39 37.72800094634731 40.0 37.0 40.0 33.0 40.0 40-41 37.62127615078245 40.0 37.0 40.0 33.0 40.0 42-43 37.52548293196732 40.0 37.0 40.0 33.0 40.0 44-45 37.43513751281114 40.0 37.0 40.0 33.0 40.0 46-47 37.28927189747172 40.0 37.0 40.0 33.0 40.0 48-49 37.15391312464831 40.0 37.0 40.0 33.0 40.0 50-51 35.73441264037346 38.5 35.0 38.5 30.0 38.5 52-53 36.06050098156079 37.0 37.0 38.5 33.0 40.0 54-55 36.76124843837175 37.0 37.0 40.0 33.0 40.0 56-57 36.73977201242284 37.0 37.0 40.0 33.0 40.0 58-59 36.60379844807635 37.0 37.0 40.0 33.0 40.0 60-61 36.46019103132997 37.0 37.0 40.0 33.0 40.0 62-63 36.30269768667739 37.0 37.0 40.0 33.0 40.0 64-65 36.095210561083206 37.0 37.0 40.0 33.0 40.0 66-67 35.84372105481915 37.0 37.0 40.0 33.0 40.0 68-69 35.60217065430463 37.0 37.0 37.0 33.0 40.0 70-71 35.375911450157886 37.0 37.0 37.0 33.0 40.0 72-73 35.1675216897122 37.0 37.0 37.0 33.0 40.0 74-75 34.944847910002466 37.0 33.0 37.0 30.0 38.5 76-77 34.75657469065257 37.0 33.0 37.0 27.0 37.0 78-79 34.58509915996159 37.0 33.0 37.0 27.0 37.0 80-81 34.42757189100199 37.0 33.0 37.0 27.0 37.0 82-83 34.31674261239128 37.0 33.0 37.0 27.0 37.0 84-85 34.217253601742 37.0 33.0 37.0 27.0 37.0 86-87 34.11452569115703 37.0 33.0 37.0 27.0 37.0 88-89 34.02831865548016 37.0 33.0 37.0 27.0 37.0 90-91 33.94365244225169 37.0 33.0 37.0 27.0 37.0 92-93 33.87022658620009 37.0 33.0 37.0 27.0 37.0 94-95 33.79478530163149 37.0 33.0 37.0 27.0 37.0 96-97 33.70546033326991 37.0 33.0 37.0 27.0 37.0 98-99 33.642623158245556 37.0 33.0 37.0 27.0 37.0 100-101 31.91046897907412 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13185311884351592 2306 2 0.26326156675146706 2306 3 0.2938343458567729 2306 4 0.3687182122713466 2306 5 0.3821932174951641 2306 6 0.4054188888069845 2306 7 0.41181412229245495 2306 8 0.40080558085987406 2306 9 0.41879490120903284 2306 10-11 0.4099627292548078 2306 12-13 0.4066558757289158 2306 14-15 0.7266380313969663 2306 16-17 0.7186672069056854 2306 18-19 0.7561386502443028 2306 20-21 0.7603328869298238 2306 22-23 0.7533782638665159 2306 24-25 0.7651951290793662 2306 26-27 0.7760994837490287 2306 28-29 0.8014397809754428 2306 30-31 0.7894742523379961 2306 32-33 0.8332350348289239 2306 34-35 0.8335860457086781 2306 36-37 0.8367252441123512 2306 38-39 0.8042180922836764 2306 40-41 0.8180237147544247 2306 42-43 0.7984316588021798 2306 44-45 0.8364153575672546 2306 46-47 0.8403881665390358 2306 48-49 0.773451150227281 2306 50-51 0.7027094174725264 2306 52-53 0.7951099452102994 2306 54-55 0.8954746287523889 2306 56-57 0.861254833565475 2306 58-59 0.8811288718437709 2306 60-61 0.804507276877338 2306 62-63 0.8204121495708065 2306 64-65 0.8366224029667748 2306 66-67 0.7809936637586006 2306 68-69 0.8495249620869103 2306 70-71 0.8476304107999866 2306 72-73 0.8268169302221011 2306 74-75 0.8522412786523077 2306 76-77 0.8486600550596464 2306 78-79 0.7788379044216995 2306 80-81 0.8390632653863364 2306 82-83 0.8884257153930619 2306 84-85 0.9115404793263338 2306 86-87 0.8741262184809173 2306 88-89 0.8553212477712151 2306 90-91 0.8554944157179207 2306 92-93 0.9127774396872113 2306 94-95 0.8721741013449815 2306 96-97 0.8464582149536071 2306 98-99 0.8702071317938973 2306 100-101 0.695640935131987 2307 1 0.18611152149385646 2307 2 0.2464250697047774 2307 3 0.2325344774840019 2307 4 0.31156673067630436 2307 5 0.3213815677592251 2307 6 0.3088737993805921 2307 7 0.3264664199078453 2307 8 0.30282142867818607 2307 9 0.30308213449666965 2307 10-11 0.30616641547407397 2307 12-13 0.3281950074897466 2307 14-15 0.5881403048509313 2307 16-17 0.5549628217030076 2307 18-19 0.5690882912184776 2307 20-21 0.5994121043024805 2307 22-23 0.6175645283982902 2307 24-25 0.6351029932580516 2307 26-27 0.6346735411576319 2307 28-29 0.6111186704920328 2307 30-31 0.624214675293743 2307 32-33 0.6559989656007872 2307 34-35 0.6938525618171241 2307 36-37 0.6923361941293535 2307 38-39 0.6406141049926859 2307 40-41 0.6863931070662872 2307 42-43 0.680409034661146 2307 44-45 0.7088935210431444 2307 46-47 0.6319288643048893 2307 48-49 0.6316979726051883 2307 50-51 0.5637983319794984 2307 52-53 0.6427221755326897 2307 54-55 0.7804210708250352 2307 56-57 0.7391999517329921 2307 58-59 0.7200195493108978 2307 60-61 0.6684039993251361 2307 62-63 0.7263734401606499 2307 64-65 0.692166794011122 2307 66-67 0.7045775706577118 2307 68-69 0.711074284503205 2307 70-71 0.6925534169208802 2307 72-73 0.6475953242376136 2307 74-75 0.6950532715886482 2307 76-77 0.6303978573595614 2307 78-79 0.7087296422526705 2307 80-81 0.6898161939233063 2307 82-83 0.6716657422273613 2307 84-85 0.7150979267425228 2307 86-87 0.7255748373248494 2307 88-89 0.7228094803553233 2307 90-91 0.6845580183746733 2307 92-93 0.7298350943235263 2307 94-95 0.7136463134234887 2307 96-97 0.7446962013900915 2307 98-99 0.7067028687583345 2307 100-101 0.6918804847285323 2201 1 0.17489557625052754 2201 2 0.22361376974160407 2201 3 0.2320724912848604 2201 4 0.3134734322831534 2201 5 0.3151601361603156 2201 6 0.3364541989114045 2201 7 0.3073471088410926 2201 8 0.2526994161685394 2201 9 0.3119322558665871 2201 10-11 0.27230915339125517 2201 12-13 0.3157921572280813 2201 14-15 0.5655253895691175 2201 16-17 0.564810353785326 2201 18-19 0.5202073700500662 2201 20-21 0.5718514340849765 2201 22-23 0.5718173576698362 2201 24-25 0.6145895603595335 2201 26-27 0.5722419794586315 2201 28-29 0.5790396872759587 2201 30-31 0.6103844420622124 2201 32-33 0.6351410806536961 2201 34-35 0.6586445577558422 2201 36-37 0.6404379121250017 2201 38-39 0.6255371526384295 2201 40-41 0.6353921136153033 2201 42-43 0.6008030621011855 2201 44-45 0.6593723800651361 2201 46-47 0.6634684504560653 2201 48-49 0.6533076603837245 2201 50-51 0.530233206417023 2201 52-53 0.6154645381238097 2201 54-55 0.7211986764571918 2201 56-57 0.6880038541583176 2201 58-59 0.6397507457317104 2201 60-61 0.6754309632862743 2201 62-63 0.7312142458251216 2201 64-65 0.7320853563945704 2201 66-67 0.7216774782117099 2201 68-69 0.6954484630633218 2201 70-71 0.6957621813511281 2201 72-73 0.6798506984981927 2201 74-75 0.7265164477107149 2201 76-77 0.7164787708764493 2201 78-79 0.701833582593288 2201 80-81 0.751098633470761 2201 82-83 0.7255714228371133 2201 84-85 0.7234755300322178 2201 86-87 0.7774761885616002 2201 88-89 0.7621932418237378 2201 90-91 0.7406804264252287 2201 92-93 0.8114107852720309 2201 94-95 0.7636830700477688 2201 96-97 0.7919366460676613 2201 98-99 0.773702415748879 2201 100-101 0.6798108489162082 1101 1 0.24352305288329035 1101 2 0.2621479639576094 1101 3 0.23604802540668857 1101 4 0.2998520350857916 1101 5 0.31741691060639 1101 6 0.33958613295816775 1101 7 0.3346915190043589 1101 8 0.32266976940241676 1101 9 0.33604353217768335 1101 10-11 0.34110857039879505 1101 12-13 0.3195739397468529 1101 14-15 0.5525955042481385 1101 16-17 0.5861345361980455 1101 18-19 0.5746599971155604 1101 20-21 0.5539674278205737 1101 22-23 0.5864222326101896 1101 24-25 0.6033502766936749 1101 26-27 0.5830681848946426 1101 28-29 0.5803166270110154 1101 30-31 0.6026350689756867 1101 32-33 0.643589352316809 1101 34-35 0.6121295002593357 1101 36-37 0.6195204611497829 1101 38-39 0.5558943368283167 1101 40-41 0.6359708323911448 1101 42-43 0.6140660265483504 1101 44-45 0.6145591686465721 1101 46-47 0.6266352771008101 1101 48-49 0.6160436364381994 1101 50-51 0.5553159109583419 1101 52-53 0.5887375648533961 1101 54-55 0.6260685715747698 1101 56-57 0.5910135755522461 1101 58-59 0.629911614107094 1101 60-61 0.6517910134621587 1101 62-63 0.6545427264788444 1101 64-65 0.6131622004178539 1101 66-67 0.6271837298357283 1101 68-69 0.6171154326450718 1101 70-71 0.6419472123541325 1101 72-73 0.6647833195147896 1101 74-75 0.6227818434623984 1101 76-77 0.6573379440745768 1101 78-79 0.6888073481337855 1101 80-81 0.6737525963549089 1101 82-83 0.6599523324907963 1101 84-85 0.6678034198170124 1101 86-87 0.6814844697167146 1101 88-89 0.6877345993945738 1101 90-91 0.6232974343393209 1101 92-93 0.6409747613079944 1101 94-95 0.6894203261281575 1101 96-97 0.6885404815027201 1101 98-99 0.6882953753581162 1101 100-101 0.6638968507704668 2304 1 0.1400328604579002 2304 2 0.2550561780228193 2304 3 0.24147279844731173 2304 4 0.3413550429504255 2304 5 0.3448922090958675 2304 6 0.3606378964090311 2304 7 0.30789174254212526 2304 8 0.36065773709511717 2304 9 0.31254636286154636 2304 10-11 0.3861471303809978 2304 12-13 0.38494700287986205 2304 14-15 0.6160601288869643 2304 16-17 0.6065770594983704 2304 18-19 0.581610858910814 2304 20-21 0.6146442668561889 2304 22-23 0.6504318525712236 2304 24-25 0.6293587942437355 2304 26-27 0.6767200984505166 2304 28-29 0.7179777403867007 2304 30-31 0.7397345299146778 2304 32-33 0.7099888473551488 2304 34-35 0.7287961280240367 2304 36-37 0.7140414092571703 2304 38-39 0.6768767062451957 2304 40-41 0.698309563407598 2304 42-43 0.7252279837076614 2304 44-45 0.7424220509582966 2304 46-47 0.7366652012002461 2304 48-49 0.737180856898398 2304 50-51 0.6622290449198047 2304 52-53 0.772729823220736 2304 54-55 0.7726588814628812 2304 56-57 0.7551057949963962 2304 58-59 0.7711598140954479 2304 60-61 0.7952101553371307 2304 62-63 0.8094862998846963 2304 64-65 0.7878562877363535 2304 66-67 0.7609159144152287 2304 68-69 0.7753058277780056 2304 70-71 0.8042848611886342 2304 72-73 0.7520858168866127 2304 74-75 0.7887096486283411 2304 76-77 0.7973604845808921 2304 78-79 0.8015651063705178 2304 80-81 0.8331494687641694 2304 82-83 0.8412198971704399 2304 84-85 0.8471547373169628 2304 86-87 0.8713389289747937 2304 88-89 0.8794665453281993 2304 90-91 0.8593350696571633 2304 92-93 0.8918151746222804 2304 94-95 0.9168617940535242 2304 96-97 0.920758049870976 2304 98-99 0.9327510353571995 2304 100-101 1.0123757237847606 2202 1 0.20350912640012098 2202 2 0.17840650007202896 2202 3 0.18306318851037418 2202 4 0.23454015870601097 2202 5 0.21239845321239414 2202 6 0.24037417829741514 2202 7 0.24193717664230974 2202 8 0.23009513389088454 2202 9 0.17930158838129984 2202 10-11 0.21614636382953023 2202 12-13 0.21882293041760192 2202 14-15 0.37016628675559815 2202 16-17 0.37336917078454945 2202 18-19 0.35613875432955666 2202 20-21 0.3996779560243766 2202 22-23 0.34706287199364994 2202 24-25 0.3683593391222999 2202 26-27 0.3685706229570087 2202 28-29 0.4113178241606761 2202 30-31 0.41429887495915807 2202 32-33 0.46265051512994404 2202 34-35 0.44641286378458034 2202 36-37 0.42775715808847536 2202 38-39 0.48214864682114467 2202 40-41 0.4481983172206654 2202 42-43 0.4019340434998 2202 44-45 0.4503306649279111 2202 46-47 0.4217550856276233 2202 48-49 0.3796916688550809 2202 50-51 0.47921152691690594 2202 52-53 0.5324720686189934 2202 54-55 0.5240331969132015 2202 56-57 0.5073872517589777 2202 58-59 0.4764194171708951 2202 60-61 0.5391741649460187 2202 62-63 0.5443234341409848 2202 64-65 0.5596454954795504 2202 66-67 0.5375981843959394 2202 68-69 0.5653727015993653 2202 70-71 0.47181992110002824 2202 72-73 0.5700752929411905 2202 74-75 0.5634343549715979 2202 76-77 0.5598413726715847 2202 78-79 0.5292555788899804 2202 80-81 0.5229188707842809 2202 82-83 0.5572095485340114 2202 84-85 0.536652887217997 2202 86-87 0.47047231784389254 2202 88-89 0.5728438966383678 2202 90-91 0.5371265427736915 2202 92-93 0.5787920930681096 2202 94-95 0.5517592268117681 2202 96-97 0.5512252204103802 2202 98-99 0.5818593766477491 2202 100-101 0.6067458286704159 1102 1 0.14368262065518422 1102 2 0.17405133883693225 1102 3 0.17530703065175146 1102 4 0.19894814158568863 1102 5 0.18728537060542294 1102 6 0.21818933638142823 1102 7 0.24143255769349992 1102 8 0.23104949416024567 1102 9 0.22105237816125367 1102 10-11 0.2033801547676788 1102 12-13 0.2375497049624542 1102 14-15 0.3528877652939286 1102 16-17 0.33345794673729046 1102 18-19 0.3137070550163443 1102 20-21 0.3488277592872606 1102 22-23 0.351937736808722 1102 24-25 0.39722635871433454 1102 26-27 0.4074298169969168 1102 28-29 0.42998003747705127 1102 30-31 0.32067815949085343 1102 32-33 0.4374755360367004 1102 34-35 0.3628446174848179 1102 36-37 0.44734488675226913 1102 38-39 0.42782139076656733 1102 40-41 0.39831786179100703 1102 42-43 0.4190577938130815 1102 44-45 0.4219881877956908 1102 46-47 0.3570266661089292 1102 48-49 0.32197809343112027 1102 50-51 0.3780316736786986 1102 52-53 0.38950965749318556 1102 54-55 0.4956846193464628 1102 56-57 0.5012626374442775 1102 58-59 0.48333986222495895 1102 60-61 0.4909521647664974 1102 62-63 0.48604506477843046 1102 64-65 0.46860149812955854 1102 66-67 0.47659718001152385 1102 68-69 0.3997050703962728 1102 70-71 0.4659376479336359 1102 72-73 0.45154125562374503 1102 74-75 0.4460079100919998 1102 76-77 0.42081049885433686 1102 78-79 0.4567490308955868 1102 80-81 0.4678181692851311 1102 82-83 0.46619778945441936 1102 84-85 0.46808907136974653 1102 86-87 0.46276547888826514 1102 88-89 0.4845214669532254 1102 90-91 0.46428011968535543 1102 92-93 0.4101539488715673 1102 94-95 0.4765767081771983 1102 96-97 0.515513541033009 1102 98-99 0.550845271310088 1102 100-101 0.587282557339531 2305 1 0.17494220470710786 2305 2 0.17226715489896804 2305 3 0.20929578283941908 2305 4 0.1866608499565956 2305 5 0.22141863102834947 2305 6 0.2699962252761452 2305 7 0.25757432135748815 2305 8 0.24987696829182937 2305 9 0.22632955315082626 2305 10-11 0.23397519472238315 2305 12-13 0.2725074097827829 2305 14-15 0.4062215802475606 2305 16-17 0.37767571158978086 2305 18-19 0.37754594873127445 2305 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sequence quality scores pass #Quality Count 2 16648.0 3 1383.0 4 1137.0 5 995.0 6 858.0 7 1135.0 8 2530.0 9 11865.0 10 43797.0 11 48663.0 12 27352.0 13 22980.0 14 24300.0 15 27106.0 16 31302.0 17 36038.0 18 41263.0 19 47167.0 20 53251.0 21 60610.0 22 68529.0 23 78292.0 24 89862.0 25 105010.0 26 124267.0 27 155139.0 28 171423.0 29 200549.0 30 250174.0 31 319089.0 32 418484.0 33 564543.0 34 784507.0 35 1210319.0 36 2503951.0 37 6674149.0 38 6702827.0 39 22191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.12672757936819 14.937059275371572 13.251786412748615 50.684426732511625 2 21.07362538992692 20.35767378282301 39.68770292017726 18.88099790707281 3 22.30417636871318 25.171633211243222 26.984188884060465 25.54000153598313 4 25.02125752485788 29.153264352312654 20.714386673146336 25.11109144968313 5 26.97853928834007 31.026038721537024 23.13811890973475 18.857303080388157 6 20.642671665456653 34.23582979867565 23.629758692853954 21.49173984301374 7 19.29443796864942 17.164491417696937 41.00732250230328 22.533748111350366 8 20.467206355646578 22.670244202931276 29.017159605381405 27.84538983604074 9 21.092307347388186 21.45159727461668 32.12426727633139 25.33182810166375 10-11 24.156021499924183 29.761436022356317 22.44522091396231 23.637321563757187 12-13 22.51095316965198 24.271345148175985 28.519082146998304 24.698619535173734 14-15 22.943271026645505 25.826699940865012 27.48576661686119 23.744262415628295 16-17 24.16636340554522 25.793397670509943 26.139135668630832 23.90110325531401 18-19 23.87557583274803 25.986292672494294 26.218804599935464 23.919326894822213 20-21 23.532020395958217 26.392979838326404 26.606912773254756 23.468086992460627 22-23 23.665779525964307 26.311901707156167 26.477939371557458 23.544379395322068 24-25 23.454825548753874 26.422169847903437 26.487822970882924 23.635181632459766 26-27 23.274956474170214 26.81313487218449 26.408125484410387 23.503783169234907 28-29 23.76011202757902 26.212103809814273 26.11388180025505 23.913902362351653 30-31 23.571131226872655 26.183428004260172 26.492706623291358 23.75273414557582 32-33 23.541849301306065 26.453159080239146 26.66740551064639 23.337586107808395 34-35 23.779500920952994 26.36040920715379 26.21532961764799 23.644760254245227 36-37 23.33425395315786 26.454514588557632 26.265792703265266 23.94543875501924 38-39 23.441163131026727 26.263542563623293 26.603451603631466 23.691842701718514 40-41 23.786905716479144 26.1956967619522 26.178852738115022 23.838544783453635 42-43 23.51924015716854 26.23162393348306 26.566436875924737 23.682699033423663 44-45 23.92741367195346 26.401943888565444 26.459621289658564 23.211021149822535 46-47 23.916620900702856 26.14021798783593 26.097940851982305 23.84522025947891 48-49 23.54507599523063 25.97318010603427 26.128952809150814 24.352791089584283 50-51 23.49493894595782 26.27788644673358 26.4283099688306 23.79886463847801 52-53 23.530206648412097 26.339159099035257 26.52492194526645 23.605712307286193 54-55 23.118565809901757 26.300857895995794 26.621214579619966 23.959361714482483 56-57 23.301947954108222 26.386256221189697 26.863468904524556 23.44832692017752 58-59 23.56564677242505 26.28781016139619 26.484875266338747 23.661667799840007 60-61 23.142865540802596 26.735682416194052 26.367194566297155 23.754257476706197 62-63 23.204639159677377 27.096634066914376 26.39684867070021 23.301878102708038 64-65 23.313110566632602 27.048110189024115 26.156349510590722 23.482429733752557 66-67 23.26024391827641 26.803333101593772 26.29465480552052 23.641768174609297 68-69 23.43833345250609 26.90280015187835 26.285084586527258 23.373781809088307 70-71 23.581994458211884 26.708029928858203 26.125502895942166 23.584472716987747 72-73 23.327909111476327 26.74032481619855 26.26180908174519 23.66995699057993 74-75 23.328796147414682 26.88830360869325 26.454952786761808 23.327947457130264 76-77 23.631276790506497 26.592781384110943 26.16012820635747 23.61581361902509 78-79 23.521424603788216 26.511087980955217 26.18717201532858 23.780315399927986 80-81 23.567159794055808 26.73680822264147 26.176552556244463 23.519479427058254 82-83 23.695405778986284 26.64605313804134 25.989399903841452 23.66914117913093 84-85 23.40389718130188 26.660194339966075 26.26032796733443 23.675580511397616 86-87 23.58998315212728 26.832576027549344 26.202158697926414 23.37528212239696 88-89 23.800469096285912 26.521623817779922 25.911065391865474 23.766841694068695 90-91 23.606696959988742 26.418580936737285 26.169322614510833 23.805399488763143 92-93 23.61919036065617 26.785438605228673 26.23211868090312 23.36325235321204 94-95 23.75279008528975 26.628856231056858 26.166266584074826 23.452087099578566 96-97 23.55612742051035 26.680841091723238 26.17745208209332 23.585579405673087 98-99 23.80931512400878 26.667643187435473 26.096003381661152 23.42703830689459 100-101 24.083060501777144 26.56184062587352 25.80025817026787 23.554840702081467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15197.0 1 8345.0 2 1203.5 3 897.5 4 930.0 5 1084.0 6 1298.0 7 1594.5 8 2018.5 9 2600.0 10 3499.5 11 4726.5 12 6402.0 13 8817.5 14 13533.0 15 18680.0 16 22373.0 17 27683.0 18 34843.5 19 43048.5 20 53142.5 21 64449.0 22 76269.0 23 89203.5 24 103312.0 25 118290.5 26 134363.5 27 151967.0 28 171276.0 29 191798.5 30 212800.5 31 235665.5 32 262932.0 33 295043.0 34 332712.5 35 375064.5 36 418911.5 37 468360.0 38 525190.0 39 584514.5 40 644385.0 41 700178.0 42 746043.0 43 770783.0 44 772973.5 45 757746.0 46 729313.0 47 692999.5 48 651258.5 49 613084.0 50 571474.5 51 527512.0 52 501359.5 53 496471.0 54 491282.5 55 481184.5 56 474265.5 57 463294.5 58 447472.5 59 428245.0 60 419213.0 61 405900.0 62 384777.0 63 361471.5 64 318909.0 65 287687.0 66 274295.0 67 255926.5 68 230416.5 69 197077.0 70 168290.0 71 142183.0 72 116823.5 73 95484.5 74 72542.0 75 52633.5 76 37926.5 77 27293.0 78 19433.0 79 13417.0 80 8782.5 81 5437.5 82 3101.0 83 1826.5 84 1172.0 85 821.5 86 686.0 87 544.0 88 420.5 89 339.5 90 253.0 91 203.0 92 178.5 93 137.5 94 104.0 95 76.5 96 49.5 97 36.0 98 22.5 99 13.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09639659878383389 2 0.08579674493767453 3 0.09060487683996393 4 0.10914029694392367 5 0.09219485491688784 6 0.09400446960503847 7 0.1018397669751049 8 0.08914381590441224 9 0.1176774765281277 10-11 0.08964038563414223 12-13 0.08325421242727819 14-15 0.1335939687786557 16-17 0.13365126528593227 18-19 0.12642713066014888 20-21 0.13824214793146478 22-23 0.1363227149377008 24-25 0.11042230629423619 26-27 0.11422536196471635 28-29 0.10358253573809957 30-31 0.09160995307177319 32-33 0.11276191367469478 34-35 0.13168408520277114 36-37 0.11844859202189109 38-39 0.12007199306139298 40-41 0.14908312457907957 42-43 0.12359572825889999 44-45 0.1222970074272985 46-47 0.13993239489612264 48-49 0.15032932361234425 50-51 0.12134445299382605 52-53 0.09275110850836421 54-55 0.09463473118508037 56-57 0.0999752431341476 58-59 0.09617696217260716 60-61 0.09644912058217071 62-63 0.08552697388258083 64-65 0.107645813045794 66-67 0.10604867290546054 68-69 0.10350136568612449 70-71 0.10379023557697703 72-73 0.10110684915285921 74-75 0.13897267839924063 76-77 0.15617834206349074 78-79 0.15542393805101634 80-81 0.1254339912006889 82-83 0.10498630016637474 84-85 0.10896363271315433 86-87 0.1152137267152366 88-89 0.11099527136700156 90-91 0.11565777464662977 92-93 0.10232678728695546 94-95 0.09890093362271253 96-97 0.10263714336803671 98-99 0.09099401561855042 100-101 0.09870039584724466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.0943685E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.104686876919764 #Duplication Level Percentage of deduplicated Percentage of total 1 82.78740161039188 47.27548646315231 2 8.726409960668818 9.96637816727253 3 3.0898491147391094 5.293345985823136 4 1.5975739460014116 3.649158397965429 5 0.9706466048630944 2.7714235219426135 6 0.5309252569964378 1.819099233349784 7 0.3591794004033726 1.4357579034872068 8 0.3224724455584334 1.4731750424039116 9 0.2601962554997432 1.3372583127173863 >10 1.1916090248937878 12.378286417593856 >50 0.10582727823825347 4.199021134719078 >100 0.05305646319942092 5.5966319989809925 >500 0.003687594547311077 1.4255986406387746 >1k 0.001128744713340372 1.0922551575562498 >5k 2.7904629827328484E-5 0.09955408999575667 >10k+ 8.394655754634648E-6 0.1875695324011324 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 39128 0.1868248113930285 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.19856104596683916 1.4324126819134264E-5 4.774708939711421E-6 2 0.19868041369033196 1.9098835758845684E-5 9.549417879422842E-6 3 0.19904329156975004 2.3873544698557106E-5 1.9098835758845684E-5 4 0.19993138743253636 2.8648253638268528E-5 2.3873544698557106E-5 5 0.2001319252080042 2.8648253638268528E-5 2.3873544698557106E-5 6 0.20072398911652845 2.8648253638268528E-5 2.8648253638268528E-5 7 0.2008672303847198 4.297238045740279E-5 3.342296257797995E-5 8 0.20119668530155987 4.774708939711421E-5 3.342296257797995E-5 9 0.201707579158109 4.774708939711421E-5 3.342296257797995E-5 10-11 0.2025479279314982 5.0134443866969924E-5 3.819767151769137E-5 12-13 0.20359597654376485 6.207121621624847E-5 4.774708939711421E-5 14-15 0.20469415959989848 6.207121621624847E-5 6.923327962581562E-5 16-17 0.20585918858118807 7.162063409567132E-5 7.639534303538274E-5 18-19 0.2071292611591513 7.162063409567132E-5 9.071946985451701E-5 20-21 0.2085091520427279 7.639534303538275E-5 9.071946985451701E-5 22-23 0.2098580073181964 8.594476091480559E-5 9.788153326408414E-5 24-25 0.2111949258213156 9.071946985451701E-5 1.0743095114350698E-4 26-27 0.2124435122090501 9.310682432437272E-5 1.122056600832184E-4 28-29 0.21378998013004874 1.0504359667365127E-4 1.1936772349278554E-4 30-31 0.21515554688680621 1.2414243243249697E-4 1.3130449584206408E-4 32-33 0.21642561946476946 1.384665592516312E-4 1.5279068607076547E-4 34-35 0.21763839553545616 1.4801597713105406E-4 1.551780405406212E-4 36-37 0.2189442784304672 1.6234010395018832E-4 1.6234010395018832E-4 38-39 0.22020957629949076 1.790515852391783E-4 1.6711481288989976E-4 40-41 0.22170406019762043 1.8382629417888974E-4 1.6950216735975548E-4 42-43 0.22298606954793293 1.8860100311860113E-4 1.7666423076932258E-4 44-45 0.2244757787371229 2.005377754678797E-4 1.8382629417888974E-4 46-47 0.225996523534421 2.1008719334730255E-4 1.9098835758845685E-4 48-49 0.22758411425687503 2.1963661122672537E-4 1.98150420998024E-4 50-51 0.22955129434003615 2.2918602910614825E-4 2.0769983887744683E-4 52-53 0.2316402295011599 2.4351015592528248E-4 2.2918602910614822E-4 54-55 0.23371722788993438 2.5783428274441677E-4 2.2918602910614822E-4 56-57 0.23615471680365704 2.5783428274441677E-4 2.3873544698557107E-4 58-59 0.2391269731186274 2.5783428274441677E-4 2.4351015592528248E-4 60-61 0.2423666131342216 2.6260899168412816E-4 2.4351015592528248E-4 62-63 0.24602881489098027 2.840951819128296E-4 2.5783428274441677E-4 64-65 0.25034753912694924 2.912572453223967E-4 2.602216372142725E-4 66-67 0.2551222480666607 3.0080666320181956E-4 2.697710550936953E-4 68-69 0.26034100493776524 3.103560810812424E-4 2.7932047297311814E-4 70-71 0.2669157791477479 3.103560810812424E-4 2.88869890852541E-4 72-73 0.27488476836812625 3.3900433471951096E-4 2.912572453223967E-4 74-75 0.284567878097861 3.652652338879237E-4 2.960319542621081E-4 76-77 0.2958242544232307 3.772020062372023E-4 2.960319542621081E-4 78-79 0.3096374873858158 3.819767151769137E-4 3.05581372141531E-4 80-81 0.327354044906615 4.0585025987547083E-4 3.199054989606652E-4 82-83 0.34989067110205296 4.13012323285038E-4 3.4377904365922234E-4 84-85 0.379092790977328 4.297238045740279E-4 3.581031704783566E-4 86-87 0.4152182388151846 4.416605769233065E-4 3.581031704783566E-4 88-89 0.45997874777050934 4.512099948027293E-4 3.7242729729749087E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGT 15805 0.0 24.676733 9 CGCCGTA 9375 0.0 23.666666 46-47 CCGTATC 10285 0.0 21.84885 48-49 GCCGTAT 9345 0.0 21.352156 48-49 CGTATCA 9405 0.0 21.081938 50-51 GTAGGGA 11125 0.0 19.986286 18-19 AAGAGCG 20140 0.0 19.548557 8 TCGTGTA 11705 0.0 18.977139 14-15 GAGTGTA 10530 0.0 18.674109 28-29 AGTGTAG 11145 0.0 18.47468 28-29 GATCGGA 24945 0.0 18.313517 2 ATCGGAA 25120 0.0 17.960413 3 CGTGTAG 11685 0.0 17.21945 16-17 TGTAGGG 11610 0.0 17.18713 18-19 TCGGGGG 11385 0.0 16.859894 38-39 GTCGTGT 11815 0.0 16.709284 14-15 TAGATCT 14225 0.0 16.578917 32-33 TAGGGAA 12605 0.0 16.244415 20-21 CGGAAGA 25395 0.0 16.213781 5 TCGGAAG 25995 0.0 16.150316 4 >>END_MODULE