FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 mx0-3.3420000000cbd3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 mx0-3.3420000000cbd3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20943685
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC391280.1868248113930285Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT158050.024.6767339
CGCCGTA93750.023.66666646-47
CCGTATC102850.021.8488548-49
GCCGTAT93450.021.35215648-49
CGTATCA94050.021.08193850-51
GTAGGGA111250.019.98628618-19
AAGAGCG201400.019.5485578
TCGTGTA117050.018.97713914-15
GAGTGTA105300.018.67410928-29
AGTGTAG111450.018.4746828-29
GATCGGA249450.018.3135172
ATCGGAA251200.017.9604133
CGTGTAG116850.017.2194516-17
TGTAGGG116100.017.1871318-19
TCGGGGG113850.016.85989438-39
GTCGTGT118150.016.70928414-15
TAGATCT142250.016.57891732-33
TAGGGAA126050.016.24441520-21
CGGAAGA253950.016.2137815
TCGGAAG259950.016.1503164
Produced by FastQC (version 0.11.2)