##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 m12-3.3420000000ccd2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18952035 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3579300059334 33.0 33.0 33.0 33.0 33.0 2 32.30085270526358 33.0 33.0 33.0 33.0 33.0 3 32.28771348301119 33.0 33.0 33.0 33.0 33.0 4 36.11579099553161 37.0 37.0 37.0 37.0 37.0 5 36.099618378712364 37.0 37.0 37.0 37.0 37.0 6 36.09591655988394 37.0 37.0 37.0 37.0 37.0 7 36.14300554003831 37.0 37.0 37.0 37.0 37.0 8 36.13358839829074 37.0 37.0 37.0 37.0 37.0 9 36.118737328207764 37.0 37.0 37.0 37.0 37.0 10-11 36.09886629588854 37.0 37.0 37.0 37.0 37.0 12-13 36.095545940053405 37.0 37.0 37.0 37.0 37.0 14-15 38.26239113636082 40.0 37.0 40.0 37.0 40.0 16-17 38.2439667296942 40.0 37.0 40.0 37.0 40.0 18-19 38.21534510673919 40.0 37.0 40.0 37.0 40.0 20-21 38.17590498856719 40.0 37.0 40.0 37.0 40.0 22-23 38.167417694194846 40.0 37.0 40.0 37.0 40.0 24-25 38.11255424549395 40.0 37.0 40.0 37.0 40.0 26-27 38.05565370684468 40.0 37.0 40.0 35.0 40.0 28-29 37.99919201288938 40.0 37.0 40.0 33.0 40.0 30-31 37.938928141489825 40.0 37.0 40.0 33.0 40.0 32-33 37.84458434147045 40.0 37.0 40.0 33.0 40.0 34-35 37.766338548868234 40.0 37.0 40.0 33.0 40.0 36-37 37.688743398795964 40.0 37.0 40.0 33.0 40.0 38-39 37.588752395191335 40.0 37.0 40.0 33.0 40.0 40-41 37.46540830575714 40.0 37.0 40.0 33.0 40.0 42-43 37.3499827591074 40.0 37.0 40.0 33.0 40.0 44-45 37.232476908152606 40.0 37.0 40.0 33.0 40.0 46-47 37.081316782076435 40.0 37.0 40.0 33.0 40.0 48-49 36.93303919605467 40.0 37.0 40.0 33.0 40.0 50-51 35.48449641423731 37.0 35.0 38.5 30.0 38.5 52-53 35.802987779412604 37.0 35.0 38.5 33.0 40.0 54-55 36.49721452076255 37.0 37.0 40.0 33.0 40.0 56-57 36.45971944437629 37.0 37.0 40.0 33.0 40.0 58-59 36.319728672936705 37.0 37.0 40.0 33.0 40.0 60-61 36.15157667764966 37.0 37.0 40.0 33.0 40.0 62-63 35.9800447550883 37.0 37.0 40.0 33.0 40.0 64-65 35.77748489805976 37.0 37.0 40.0 33.0 40.0 66-67 35.532287218760416 37.0 37.0 37.0 33.0 40.0 68-69 35.30676650291116 37.0 37.0 37.0 30.0 40.0 70-71 35.09232829086692 37.0 33.0 37.0 27.0 40.0 72-73 34.895460513870944 37.0 33.0 37.0 27.0 40.0 74-75 34.67932361880927 37.0 33.0 37.0 27.0 37.0 76-77 34.50085787621224 37.0 33.0 37.0 27.0 37.0 78-79 34.33256394366093 37.0 33.0 37.0 27.0 37.0 80-81 34.18118447438494 37.0 33.0 37.0 27.0 37.0 82-83 34.0745561624385 37.0 33.0 37.0 27.0 37.0 84-85 33.97780264757848 37.0 33.0 37.0 27.0 37.0 86-87 33.87304075789223 37.0 33.0 37.0 27.0 37.0 88-89 33.785639484097615 37.0 33.0 37.0 27.0 37.0 90-91 33.69769320286713 37.0 33.0 37.0 27.0 37.0 92-93 33.61724959878978 37.0 33.0 37.0 27.0 37.0 94-95 33.53224598308309 37.0 33.0 37.0 27.0 37.0 96-97 33.439488160506244 37.0 33.0 37.0 27.0 37.0 98-99 33.368741905552625 37.0 33.0 37.0 27.0 37.0 100-101 31.549269854134394 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19931222102628965 2306 2 0.30093765733828093 2306 3 0.34006460817008133 2306 4 0.4443206554402934 2306 5 0.4003716524676406 2306 6 0.45774681849215426 2306 7 0.4432865662034473 2306 8 0.46945999677804906 2306 9 0.47507753471379743 2306 10-11 0.5069123520186949 2306 12-13 0.46831933939242987 2306 14-15 0.7956720558403205 2306 16-17 0.7880723274908874 2306 18-19 0.8030921548926386 2306 20-21 0.8081854763389416 2306 22-23 0.8111389486534932 2306 24-25 0.8166183669366873 2306 26-27 0.8344688839583938 2306 28-29 0.872168088384548 2306 30-31 0.8337445119998961 2306 32-33 0.8537589189463048 2306 34-35 0.8368623656164473 2306 36-37 0.871479167535341 2306 38-39 0.8275268824417452 2306 40-41 0.8693415637085309 2306 42-43 0.8483242122740151 2306 44-45 0.8922489750760931 2306 46-47 0.8943124155569322 2306 48-49 0.8165013706732509 2306 50-51 0.745610574449934 2306 52-53 0.8183406264299222 2306 54-55 0.8974552456803124 2306 56-57 0.9196679708987219 2306 58-59 0.9395471003256617 2306 60-61 0.8408750281305473 2306 62-63 0.8403848306814794 2306 64-65 0.8521497968476126 2306 66-67 0.8259757370221266 2306 68-69 0.8984009475754817 2306 70-71 0.8970986516248729 2306 72-73 0.8627831373639694 2306 74-75 0.8697933285551045 2306 76-77 0.8992785090589663 2306 78-79 0.8770809890129172 2306 80-81 0.9135222796744884 2306 82-83 0.9762862120661069 2306 84-85 0.9791656250565453 2306 86-87 0.9427959369662133 2306 88-89 0.9307602114823297 2306 90-91 0.9726409088679375 2306 92-93 0.9645849299888667 2306 94-95 0.9493306546841609 2306 96-97 0.8849375518835814 2306 98-99 0.8816907021524543 2306 100-101 0.68992857562829 2307 1 0.19135463905817574 2307 2 0.24177951970739997 2307 3 0.2496130660650664 2307 4 0.3628411312544628 2307 5 0.4028140639489237 2307 6 0.370980200736561 2307 7 0.3648241415880946 2307 8 0.36011134682115653 2307 9 0.34725326151476565 2307 10-11 0.3553051714592499 2307 12-13 0.3832608700476712 2307 14-15 0.6352266957503474 2307 16-17 0.6041712545307192 2307 18-19 0.6306926044929284 2307 20-21 0.6542264156370123 2307 22-23 0.666173762075033 2307 24-25 0.6626791393305922 2307 26-27 0.6818688730667688 2307 28-29 0.7031891684595664 2307 30-31 0.6901151702885002 2307 32-33 0.687152961501468 2307 34-35 0.7368135324219551 2307 36-37 0.747163461854143 2307 38-39 0.6656953061381756 2307 40-41 0.6772432570296587 2307 42-43 0.7051519935725494 2307 44-45 0.7657907201719851 2307 46-47 0.7316180369380376 2307 48-49 0.6989349684770971 2307 50-51 0.6046914794555462 2307 52-53 0.6782561944866714 2307 54-55 0.8308795817969141 2307 56-57 0.8123929444448308 2307 58-59 0.7719900608100332 2307 60-61 0.7599243938705769 2307 62-63 0.743000939841238 2307 64-65 0.7482784789851138 2307 66-67 0.8089601347582587 2307 68-69 0.7993207153179185 2307 70-71 0.7905387663658558 2307 72-73 0.7546028527981861 2307 74-75 0.7721956699600412 2307 76-77 0.7330996633396865 2307 78-79 0.7594058732975597 2307 80-81 0.7761707392594559 2307 82-83 0.7647391227652847 2307 84-85 0.8099860857390482 2307 86-87 0.8026879684226103 2307 88-89 0.7544520045244241 2307 90-91 0.7101837955423846 2307 92-93 0.7620921390538768 2307 94-95 0.7769918786093442 2307 96-97 0.8396056468672555 2307 98-99 0.7820196595845346 2307 100-101 0.8083385550819848 2201 1 0.2592968152302433 2201 2 0.294813092588214 2201 3 0.33377822536311186 2201 4 0.42225282825674526 2201 5 0.41457985395219765 2201 6 0.3969553028897721 2201 7 0.376923674948884 2201 8 0.3443532167704646 2201 9 0.39320551401575443 2201 10-11 0.3893906283670461 2201 12-13 0.44298137597314025 2201 14-15 0.7132065984979974 2201 16-17 0.7304960212559948 2201 18-19 0.6641841953148173 2201 20-21 0.7162479462507747 2201 22-23 0.75611906462089 2201 24-25 0.7480312248786518 2201 26-27 0.7358396904154958 2201 28-29 0.7479601132304268 2201 30-31 0.7466406352618122 2201 32-33 0.8168760776962714 2201 34-35 0.817820729722996 2201 36-37 0.8315392779728299 2201 38-39 0.8136973807774623 2201 40-41 0.8543500214746871 2201 42-43 0.8421983475606893 2201 44-45 0.8842116512428362 2201 46-47 0.8723998450368313 2201 48-49 0.8730930026401396 2201 50-51 0.7918250479424458 2201 52-53 0.8919541413897392 2201 54-55 0.9780171873623473 2201 56-57 0.9463113095196221 2201 58-59 0.8682113843923176 2201 60-61 0.961499281703631 2201 62-63 0.9739890086210465 2201 64-65 1.0080261425096992 2201 66-67 0.9671793256486723 2201 68-69 0.9179648539672343 2201 70-71 0.933968869547563 2201 72-73 0.9424656265401694 2201 74-75 0.9418997379636949 2201 76-77 0.9408395417382778 2201 78-79 0.971190155602514 2201 80-81 0.9790013122319863 2201 82-83 0.9485351987071269 2201 84-85 0.9356712322975014 2201 86-87 0.9476546761860476 2201 88-89 0.924003402764022 2201 90-91 0.8908787761159331 2201 92-93 0.9364584188243583 2201 94-95 0.914663080807351 2201 96-97 0.9319813540357913 2201 98-99 0.9108244966826433 2201 100-101 0.8785779458323582 1101 1 0.257948836538084 1101 2 0.2777212167697982 1101 3 0.24850131037231193 1101 4 0.3648441570725538 1101 5 0.3352223600313877 1101 6 0.37671522072974994 1101 7 0.36106280572014526 1101 8 0.3556200182791258 1101 9 0.3713261604784037 1101 10-11 0.3738504023022884 1101 12-13 0.354144542754959 1101 14-15 0.5903110089164798 1101 16-17 0.6112079301713678 1101 18-19 0.5988842741103753 1101 20-21 0.64313945666877 1101 22-23 0.6312108138795125 1101 24-25 0.6450640086671839 1101 26-27 0.6295864613040791 1101 28-29 0.6216282166980207 1101 30-31 0.6325817776919038 1101 32-33 0.6694835922280404 1101 34-35 0.6753138997980912 1101 36-37 0.6429259404986212 1101 38-39 0.6067448881851121 1101 40-41 0.6582169841642767 1101 42-43 0.6948493276214052 1101 44-45 0.6928942845012003 1101 46-47 0.7129274125435359 1101 48-49 0.7092215715027663 1101 50-51 0.6522493631983295 1101 52-53 0.6690492151294976 1101 54-55 0.69775885237366 1101 56-57 0.6761781418441402 1101 58-59 0.6961400346534603 1101 60-61 0.7777472437179043 1101 62-63 0.7926391452860173 1101 64-65 0.7574688645130436 1101 66-67 0.761792875805682 1101 68-69 0.7087967684314336 1101 70-71 0.7285394298350525 1101 72-73 0.7460989258357742 1101 74-75 0.7219358626350214 1101 76-77 0.7675469650821185 1101 78-79 0.8171670236084765 1101 80-81 0.7802369126601434 1101 82-83 0.7655891799426868 1101 84-85 0.8019918819487941 1101 86-87 0.7599415728841308 1101 88-89 0.8005570053001918 1101 90-91 0.711673360255439 1101 92-93 0.7395479219160137 1101 94-95 0.7675021497852939 1101 96-97 0.7573564925830567 1101 98-99 0.7851927077833736 1101 100-101 0.814066358487981 2304 1 0.13152843447826967 2304 2 0.23283757949172212 2304 3 0.2502487029870295 2304 4 0.34263136093401414 2304 5 0.36450038129349593 2304 6 0.35445978496881025 2304 7 0.32992642073830325 2304 8 0.37424501384559505 2304 9 0.3261547755804486 2304 10-11 0.38367539834968056 2304 12-13 0.3826679544306941 2304 14-15 0.6313808410502588 2304 16-17 0.6392357366945234 2304 18-19 0.6135747534739124 2304 20-21 0.6704890248005029 2304 22-23 0.7030965081884304 2304 24-25 0.6663556778389221 2304 26-27 0.6899722093740124 2304 28-29 0.7078047911634968 2304 30-31 0.7557516007846559 2304 32-33 0.7774655850212184 2304 34-35 0.7872180941894911 2304 36-37 0.7708352893900638 2304 38-39 0.7446914353803606 2304 40-41 0.8090283675395398 2304 42-43 0.8182987189590136 2304 44-45 0.8064485934169099 2304 46-47 0.8144710318480008 2304 48-49 0.8424295037428564 2304 50-51 0.7781859828547582 2304 52-53 0.9027254035339425 2304 54-55 0.9292396467305153 2304 56-57 0.8487266940404155 2304 58-59 0.849782304580252 2304 60-61 0.8506774630407037 2304 62-63 0.8714529311507988 2304 64-65 0.8429479848244767 2304 66-67 0.8459949448912312 2304 68-69 0.8798451370229046 2304 70-71 0.8812590213248797 2304 72-73 0.8697317155207998 2304 74-75 0.8952694040859797 2304 76-77 0.8976093871939952 2304 78-79 0.8707096466444142 2304 80-81 0.9112898194384869 2304 82-83 0.9469593702644801 2304 84-85 0.9638200272802422 2304 86-87 0.9719213314631077 2304 88-89 0.9752643052721623 2304 90-91 0.942805553535301 2304 92-93 0.9742547449703096 2304 94-95 0.9652101285968087 2304 96-97 1.016146185907445 2304 98-99 1.0354353974912343 2304 100-101 1.1647474378004112 2202 1 0.2192361126251967 2202 2 0.2396412152013525 2202 3 0.24796333921563019 2202 4 0.2795285218510628 2202 5 0.29166809105328895 2202 6 0.30990720688544116 2202 7 0.27723874030451157 2202 8 0.27486227391342055 2202 9 0.23014910610121575 2202 10-11 0.280572319806339 2202 12-13 0.27489035305274 2202 14-15 0.4691569533900548 2202 16-17 0.4577555868244829 2202 18-19 0.44977607426969257 2202 20-21 0.4776121483948472 2202 22-23 0.4746779314769469 2202 24-25 0.4735329970700306 2202 26-27 0.441860115591588 2202 28-29 0.5075290956700087 2202 30-31 0.5063319689246342 2202 32-33 0.5495330215190606 2202 34-35 0.5269812838469434 2202 36-37 0.5045886425282191 2202 38-39 0.5419558511363931 2202 40-41 0.5434885082726453 2202 42-43 0.5152697438181519 2202 44-45 0.5202211019877652 2202 46-47 0.5115748209136441 2202 48-49 0.47437269465263654 2202 50-51 0.5511569404293297 2202 52-53 0.5979470993876888 2202 54-55 0.6386698637780341 2202 56-57 0.6016085930227746 2202 58-59 0.5766002988258307 2202 60-61 0.6106231013394634 2202 62-63 0.6090845289490616 2202 64-65 0.6332070859030807 2202 66-67 0.5759379570645038 2202 68-69 0.6097316867742819 2202 70-71 0.5393849659133352 2202 72-73 0.5997870558840575 2202 74-75 0.5976724524506238 2202 76-77 0.6271501673348538 2202 78-79 0.5776415793961291 2202 80-81 0.6052067333058062 2202 82-83 0.6493899495832096 2202 84-85 0.6320536466084619 2202 86-87 0.6210471754891813 2202 88-89 0.6351192227361935 2202 90-91 0.6159019012746327 2202 92-93 0.6381494979174676 2202 94-95 0.6464046310876839 2202 96-97 0.6320298308485874 2202 98-99 0.568967962263585 2202 100-101 0.6221241909333948 1102 1 0.13303155673382605 1102 2 0.19309862229522423 1102 3 0.16925079265186582 1102 4 0.1723731245579998 1102 5 0.18837526843003616 1102 6 0.21253355260368778 1102 7 0.23505462932516252 1102 8 0.21666470864972354 1102 9 0.2068703888841128 1102 10-11 0.21531666825162432 1102 12-13 0.2279608588520503 1102 14-15 0.38427722717232626 1102 16-17 0.3770882513586642 1102 18-19 0.3202963801726355 1102 20-21 0.3490545512174066 1102 22-23 0.34703096885539253 1102 24-25 0.3708743594914239 1102 26-27 0.3965373638062957 1102 28-29 0.4301564119676229 1102 30-31 0.3278168690188821 1102 32-33 0.45205827700464596 1102 34-35 0.39626276688031936 1102 36-37 0.49863738328986784 1102 38-39 0.4360866594449675 1102 40-41 0.4399626554230167 1102 42-43 0.47257574931335 1102 44-45 0.48452720835549457 1102 46-47 0.3938575941886242 1102 48-49 0.4122266074598073 1102 50-51 0.43211155696794634 1102 52-53 0.4180989786840925 1102 54-55 0.5461164255778428 1102 56-57 0.5556153804167892 1102 58-59 0.540086982024718 1102 60-61 0.5169718999134858 1102 62-63 0.5042538050805092 1102 64-65 0.5070088469650074 1102 66-67 0.5113581476869626 1102 68-69 0.4638821128439261 1102 70-71 0.5176449835679833 1102 72-73 0.491902352674515 1102 74-75 0.5170316776633115 1102 76-77 0.532045311176077 1102 78-79 0.563005253680636 1102 80-81 0.566826662649639 1102 82-83 0.5450175881481627 1102 84-85 0.5026285215500721 1102 86-87 0.48214628926425007 1102 88-89 0.48714203685924673 1102 90-91 0.5364171579691472 1102 92-93 0.4417118144812662 1102 94-95 0.5284550830909254 1102 96-97 0.5424015506517748 1102 98-99 0.5672452490111866 1102 100-101 0.5875848680758828 2305 1 0.18502275177197447 2305 2 0.17721886494916106 2305 3 0.20880878580705087 2305 4 0.2107434459783093 2305 5 0.23945546381246174 2305 6 0.2420229760409498 2305 7 0.25803149797553715 2305 8 0.27112256616973696 2305 9 0.23411838497161597 2305 10-11 0.25737031388246834 2305 12-13 0.264152034090948 2305 14-15 0.4120682743576154 2305 16-17 0.3642749558861169 2305 18-19 0.35671010031191486 2305 20-21 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#Quality Count 2 15083.0 3 1237.0 4 1074.0 5 887.0 6 783.0 7 1660.0 8 3180.0 9 8049.0 10 21345.0 11 26599.0 12 21887.0 13 22641.0 14 25593.0 15 28741.0 16 33538.0 17 38840.0 18 43995.0 19 49854.0 20 56187.0 21 62945.0 22 70677.0 23 81073.0 24 92284.0 25 107940.0 26 128207.0 27 160807.0 28 177803.0 29 203842.0 30 251011.0 31 320024.0 32 417719.0 33 559496.0 34 772558.0 35 1201842.0 36 2515857.0 37 6208408.0 38 5214844.0 39 3525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.366077195390854 12.240302989154761 11.14952576476278 55.24409405069161 2 21.830551012708657 18.99294519700089 39.83939481252184 19.337108977768615 3 24.579749051981548 23.575405611204538 23.67543329574109 28.16941204107282 4 27.928156920066698 28.10487946171463 17.275357394876472 26.691606223342195 5 28.870498440753572 29.52912801823257 21.257392669146693 20.342980871867162 6 21.30894457767083 32.43237133941829 22.795940120270277 23.462743962640605 7 19.425162006371714 14.572563521302751 41.06264475253269 24.939629719792848 8 21.126012124100445 19.7933549924907 27.446396331737677 31.63423655167118 9 22.394983646390557 19.335088452757418 30.471285276320902 27.798642624531123 10-11 25.57643975168591 27.85468376599339 20.78614030222077 25.782736180099935 12-13 23.517963590805955 22.140625272708007 27.316702130864616 27.024709005621418 14-15 24.323707437042703 23.62073175029404 25.85687289164224 26.19868792102102 16-17 25.49000613073165 23.893514155089836 24.465854027931904 26.150625686246602 18-19 25.117929674354126 24.12032715156145 24.60662988562586 26.15511328845856 20-21 24.979851519715606 24.415477435630674 24.915558145795583 25.68911289885814 22-23 25.33714853271344 24.276858019463784 24.574524305635546 25.81146914218723 24-25 24.9983948349897 24.508319667311028 24.54474390533174 25.948541592367537 26-27 24.724757664957277 24.849936909861302 24.815812002823282 25.60949342235814 28-29 25.16696763201646 24.343247970728715 24.523996202913818 25.965788194341005 30-31 25.11400807325247 24.145538881574833 24.693685493833943 26.046767551338757 32-33 25.187729166900525 24.361920772714637 24.76775707136504 25.6825929890198 34-35 25.366230689763814 24.52186347038446 24.264523098737982 25.84738274111375 36-37 24.92481440829027 24.52628897274026 24.503568012258015 26.045328606711454 38-39 25.042793038909196 24.37501744990283 24.82853134459515 25.753658166592825 40-41 25.442895457489804 24.206859776220018 24.41713504595227 25.933109720337903 42-43 25.170951959482128 24.140167201161056 24.740334571906434 25.94854626745038 44-45 25.485390725167463 24.291741782391043 24.665245749100905 25.557621743340597 46-47 25.38265377899858 24.16899314390761 24.488563153160506 25.9597899239333 48-49 25.16776291792237 24.138188878376685 24.447819670620753 26.246228533080195 50-51 25.289402744013127 24.3324227829454 24.660355636336835 25.71781883670463 52-53 25.381923933413947 24.37163034118237 24.546849298862742 25.699596426540943 54-55 25.006247975217665 24.14623504829954 24.668001715842934 26.179515260639857 56-57 25.01908690768856 24.470479928442913 24.986229169125977 25.52420399474255 58-59 25.302186168291747 24.466260389192737 24.51002854251891 25.721524899996613 60-61 24.999978213524308 24.67457277183309 24.440406449546785 25.885042565095812 62-63 25.073387360738813 24.807357187873862 24.58227057689917 25.53698487448815 64-65 25.23621482013139 24.779119242701185 24.27260392987257 25.71206200729485 66-67 25.083279500402227 24.669755435629458 24.37647996543424 25.870485098534076 68-69 25.197120395197082 24.81166210841832 24.49105516994204 25.500162326442556 70-71 25.383169864409066 24.682528018292356 24.251874323501166 25.682427793797412 72-73 25.20442886917984 24.58676495358416 24.390439716241634 25.81836646099437 74-75 25.29169101305428 24.72790278180634 24.542875960116422 25.43753024502296 76-77 25.458629004521814 24.63676280555978 24.245040978763676 25.659567211154734 78-79 25.282633194272535 24.57288434107295 24.34043402791861 25.804048436735904 80-81 25.378118310152033 24.76547807709695 24.348641493806053 25.507762118944967 82-83 25.566346230079763 24.66964336034852 24.093154352816324 25.670856056755397 84-85 25.28343952232425 24.58576336549935 24.32040291537568 25.810394196800722 86-87 25.387427970607746 24.84420341512939 24.360806248482263 25.407562365780606 88-89 25.52532663673605 24.66745364714477 24.11748027673646 25.689739439382713 90-91 25.45191559725584 24.498524616780312 24.208397744414977 25.84116204154887 92-93 25.476863066671157 24.727174532372295 24.400020335227545 25.395942065729 94-95 25.647482475109168 24.617521048420844 24.149032181310016 25.585964295159975 96-97 25.376081666586558 24.644353994883954 24.24862895145355 25.730935387075938 98-99 25.543345338929456 24.73030979339027 24.343645696245446 25.38269917143483 100-101 25.853755294249503 24.60330612101215 23.968245739743526 25.57469284499482 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13065.0 1 7088.0 2 817.0 3 475.0 4 395.0 5 386.0 6 416.5 7 389.0 8 383.5 9 400.0 10 403.5 11 430.0 12 441.0 13 469.5 14 527.0 15 586.5 16 662.5 17 779.5 18 939.5 19 1184.0 20 1535.5 21 2083.5 22 2860.5 23 3976.5 24 5638.0 25 8131.5 26 11684.0 27 16646.0 28 23387.5 29 32246.5 30 43250.5 31 57640.0 32 76539.5 33 100982.0 34 132847.0 35 174039.5 36 224434.5 37 287664.5 38 361334.0 39 437357.5 40 516464.5 41 595424.0 42 665592.5 43 715387.5 44 746346.5 45 755022.5 46 744923.5 47 729173.0 48 700946.5 49 670796.0 50 643483.5 51 608470.5 52 580621.5 53 566192.5 54 559652.0 55 552950.5 56 544246.0 57 533734.0 58 514895.5 59 490176.0 60 468995.5 61 448955.5 62 428455.0 63 404471.0 64 365504.0 65 334312.5 66 316749.0 67 294691.0 68 264152.5 69 229333.0 70 198718.0 71 169036.5 72 139702.5 73 113906.5 74 90768.5 75 69439.5 76 49196.5 77 33870.5 78 23237.0 79 16118.0 80 10862.5 81 6605.0 82 3976.0 83 2467.0 84 1569.0 85 1057.0 86 779.5 87 586.5 88 488.5 89 381.5 90 294.0 91 246.5 92 183.5 93 138.0 94 110.0 95 75.5 96 52.5 97 33.5 98 17.5 99 9.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09649623378175483 2 0.08591689494030588 3 0.09116171429611648 4 0.10903314604473874 5 0.0926971694596385 6 0.09329341149908175 7 0.10179381791981705 8 0.08894559344154862 9 0.11750189359612305 10-11 0.08919094967901864 12-13 0.08337627067489059 14-15 0.13337881657563422 16-17 0.13326537229379323 18-19 0.12560656414997123 20-21 0.13871861253949772 22-23 0.1361700735567447 24-25 0.11059234536027397 26-27 0.1138690383380993 28-29 0.10354033221234554 30-31 0.09186876237828813 32-33 0.11277153086726571 34-35 0.13171144945648317 36-37 0.11807439148355309 38-39 0.12054905977115388 40-41 0.1491607629470925 42-43 0.12346167575144305 44-45 0.1223430623677088 46-47 0.14037806494131105 48-49 0.1505352855247471 50-51 0.12139857276540486 52-53 0.09202178024681783 54-55 0.0944542367086173 56-57 0.0997254384555537 58-59 0.09591054469876191 60-61 0.09640389541281451 62-63 0.08554754146454457 64-65 0.10725497288285929 66-67 0.10555594689435725 68-69 0.10292562249911422 70-71 0.10308919332409422 72-73 0.1011289816634467 74-75 0.1393016633833781 76-77 0.15592520803174964 78-79 0.15563763996847832 80-81 0.1247042863734686 82-83 0.10470643390010624 84-85 0.10877723685081839 86-87 0.11472118957146289 88-89 0.11095114587958498 90-91 0.11538866406694585 92-93 0.10221857441694256 94-95 0.09818206857469394 96-97 0.10260111908826679 98-99 0.09104035529693777 100-101 0.09834563939967397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.8952035E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.618910186390615 #Duplication Level Percentage of deduplicated Percentage of total 1 72.32197192905736 35.8853742965056 2 14.028161559164998 13.921241769687706 3 5.093158240499052 7.581508839011931 4 2.7094384682915162 5.37757536054834 5 1.503621722728992 3.7304035607197896 6 0.9160223488341198 2.727121839331606 7 0.6960873525771223 2.4177367080584653 8 0.4467512209320964 1.7733846965672033 9 0.3444224031903779 1.5380877861075761 >10 1.7953608368771614 15.585056248770057 >50 0.09154029305992213 3.1554631781347813 >100 0.04988142583962282 4.461746547932736 >500 0.0027840503692481366 0.9124799654723669 >1k 7.646353721340231E-4 0.5925326887993334 >5k 2.2874871895368605E-5 0.07295580057626494 >10k+ 1.0638334373430258E-5 0.2673307137762977 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 50644 0.2672219632350827 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.28480318868132104 3.1658869350969435E-5 0.0 2 0.28525168933045975 3.1658869350969435E-5 5.276478225161573E-6 3 0.28706679783991534 3.693534757613101E-5 5.276478225161573E-6 4 0.2933510834060828 3.693534757613101E-5 5.276478225161573E-6 5 0.29457522635432026 3.693534757613101E-5 5.276478225161573E-6 6 0.29789940763617206 6.331773870193887E-5 2.110591290064629E-5 7 0.29868560289172114 7.387069515226202E-5 2.110591290064629E-5 8 0.30028965227217025 7.914717337742359E-5 2.110591290064629E-5 9 0.302827638298473 1.0025308627806987E-4 2.638239112580786E-5 10-11 0.3079220780248665 1.1872076006613538E-4 2.638239112580786E-5 12-13 0.3133594888358955 1.4246491207936247E-4 2.638239112580786E-5 14-15 0.319108211862209 1.5565610764226639E-4 3.693534757613101E-5 16-17 0.3245588138688009 2.2161208545678606E-4 5.0126543139034935E-5 18-19 0.32990388631088957 2.2688856368194762E-4 5.276478225161572E-5 20-21 0.335338658882806 2.2952680279452842E-4 5.276478225161572E-5 22-23 0.3395149913980214 2.5063271569517466E-4 5.8041260476777295E-5 24-25 0.3433061410028 2.638239112580786E-4 6.595597781451965E-5 26-27 0.3471210347595918 2.7701510682098254E-4 7.123245603968124E-5 28-29 0.35050853378014546 2.902063023838865E-4 7.914717337742359E-5 30-31 0.3532707701310176 3.033974979467904E-4 9.233836894032752E-5 32-33 0.35569267363636675 3.3241812818517907E-4 1.319119556290393E-4 34-35 0.35797475046874916 3.4297108463550224E-4 1.4246491207936245E-4 36-37 0.36020406251887993 3.482475628606638E-4 1.4246491207936245E-4 38-39 0.3624518422427987 3.5880051931098695E-4 1.4246491207936245E-4 40-41 0.36474183379251884 3.5880051931098695E-4 1.5565610764226639E-4 42-43 0.3669368487341861 3.7199171487389083E-4 1.9786793344355895E-4 44-45 0.36894454869886006 4.0101234511227954E-4 2.2688856368194762E-4 46-47 0.37108943709738823 4.1156530156260266E-4 2.2688856368194762E-4 48-49 0.37356938186321414 4.19480018900345E-4 2.3480328101968998E-4 50-51 0.37603877367258975 4.405859318009913E-4 2.3744152013227077E-4 52-53 0.3782971063529589 4.511388882513144E-4 2.4007975924485154E-4 54-55 0.3806451391631558 4.590536055890568E-4 2.479944765825939E-4 56-57 0.38318576342857114 4.6960656203937995E-4 2.479944765825939E-4 58-59 0.38558392278190706 4.775212793771223E-4 2.664621503706594E-4 60-61 0.38821424717715014 5.012654313903493E-4 2.7437686770840177E-4 62-63 0.39095273937600894 5.487537354168035E-4 2.7437686770840177E-4 64-65 0.39368595509664267 5.619449309797074E-4 2.7437686770840177E-4 66-67 0.3962292176011705 5.751361265426114E-4 2.7701510682098254E-4 68-69 0.39898353923470486 5.856890829929345E-4 2.849298241587249E-4 70-71 0.402204829191166 5.962420394432577E-4 2.849298241587249E-4 72-73 0.40580866381895137 5.988802785558384E-4 2.9284454149646724E-4 74-75 0.40968687531444514 6.226244305690655E-4 2.9548278060904806E-4 76-77 0.41404260808931603 6.358156261319695E-4 2.9548278060904806E-4 78-79 0.41909747422902077 6.516450608074542E-4 2.9812101972162883E-4 80-81 0.42511793588393015 6.885804083835852E-4 3.0867397617195195E-4 82-83 0.4328664441575799 6.938568866087468E-4 3.2450341084743666E-4 84-85 0.4426806936563804 6.991333648339083E-4 3.271416499600175E-4 86-87 0.45477701998756337 7.149627995093931E-4 3.271416499600175E-4 88-89 0.46911321132532735 7.360687124100393E-4 3.3769460641034065E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGT 16295 0.0 31.688301 9 AAGAGCG 18615 0.0 27.707008 8 ATCGGAA 20310 0.0 26.656132 3 GCCGTAT 10660 0.0 24.17755 48-49 GAGTGTA 10725 0.0 23.759272 28-29 AGATCGG 21945 0.0 23.37047 1 GATCGGA 23560 0.0 23.138947 2 TCGGAAG 22910 0.0 22.968151 4 CCGTATC 11990 0.0 22.446594 48-49 TGTAGGG 12265 0.0 20.897825 18-19 TCGTGTA 12700 0.0 20.78159 14-15 CGTGTAG 12455 0.0 20.63663 16-17 CGGAAGA 25425 0.0 20.621082 5 AGTGTAG 12825 0.0 20.313276 28-29 GTAGGGA 13175 0.0 20.301819 18-19 GTCGTGT 12765 0.0 20.210518 14-15 CGCCGTA 13370 0.0 19.935282 46-47 TTAAAAA 19560 0.0 19.785566 56-57 CGTATCA 13085 0.0 19.453917 50-51 GTGTAGA 13840 0.0 18.669796 30-31 >>END_MODULE