FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 m12-3.3420000000ccd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 m12-3.3420000000ccd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18952035
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC506440.2672219632350827Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT162950.031.6883019
AAGAGCG186150.027.7070088
ATCGGAA203100.026.6561323
GCCGTAT106600.024.1775548-49
GAGTGTA107250.023.75927228-29
AGATCGG219450.023.370471
GATCGGA235600.023.1389472
TCGGAAG229100.022.9681514
CCGTATC119900.022.44659448-49
TGTAGGG122650.020.89782518-19
TCGTGTA127000.020.7815914-15
CGTGTAG124550.020.6366316-17
CGGAAGA254250.020.6210825
AGTGTAG128250.020.31327628-29
GTAGGGA131750.020.30181918-19
GTCGTGT127650.020.21051814-15
CGCCGTA133700.019.93528246-47
TTAAAAA195600.019.78556656-57
CGTATCA130850.019.45391750-51
GTGTAGA138400.018.66979630-31
Produced by FastQC (version 0.11.2)