##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 kx24-2.3420000000cdfb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26285590 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38179907698477 33.0 33.0 33.0 33.0 33.0 2 32.32810798616276 33.0 33.0 33.0 33.0 33.0 3 32.30770810166331 33.0 33.0 33.0 33.0 33.0 4 36.141084069256195 37.0 37.0 37.0 37.0 37.0 5 36.13092093424572 37.0 37.0 37.0 37.0 37.0 6 36.12822158452597 37.0 37.0 37.0 37.0 37.0 7 36.176435720103676 37.0 37.0 37.0 37.0 37.0 8 36.16124804503152 37.0 37.0 37.0 37.0 37.0 9 36.14397013725011 37.0 37.0 37.0 37.0 37.0 10-11 36.12222970836873 37.0 37.0 37.0 37.0 37.0 12-13 36.11823514328573 37.0 37.0 37.0 37.0 37.0 14-15 38.30353864988383 40.0 37.0 40.0 37.0 40.0 16-17 38.28366696353402 40.0 37.0 40.0 37.0 40.0 18-19 38.26129192839119 40.0 37.0 40.0 37.0 40.0 20-21 38.22421450307944 40.0 37.0 40.0 37.0 40.0 22-23 38.20991351154758 40.0 37.0 40.0 37.0 40.0 24-25 38.16785297571788 40.0 37.0 40.0 37.0 40.0 26-27 38.111316732856295 40.0 37.0 40.0 37.0 40.0 28-29 38.06130073549804 40.0 37.0 40.0 37.0 40.0 30-31 38.01496196204841 40.0 37.0 40.0 35.0 40.0 32-33 37.92573446135316 40.0 37.0 40.0 33.0 40.0 34-35 37.84623025391479 40.0 37.0 40.0 33.0 40.0 36-37 37.77673105302183 40.0 37.0 40.0 33.0 40.0 38-39 37.68586253913266 40.0 37.0 40.0 33.0 40.0 40-41 37.57121329595417 40.0 37.0 40.0 33.0 40.0 42-43 37.47269490622048 40.0 37.0 40.0 33.0 40.0 44-45 37.36555704094906 40.0 37.0 40.0 33.0 40.0 46-47 37.23254967835989 40.0 37.0 40.0 33.0 40.0 48-49 37.08381946153767 40.0 37.0 40.0 33.0 40.0 50-51 35.659026333439726 38.5 35.0 38.5 30.0 38.5 52-53 35.99001764845301 37.0 37.0 38.5 33.0 40.0 54-55 36.6760606666999 37.0 37.0 40.0 33.0 40.0 56-57 36.64909863160766 37.0 37.0 40.0 33.0 40.0 58-59 36.51602018444326 37.0 37.0 40.0 33.0 40.0 60-61 36.35722776243561 37.0 37.0 40.0 33.0 40.0 62-63 36.186380180167156 37.0 37.0 40.0 33.0 40.0 64-65 35.984954417991 37.0 37.0 40.0 33.0 40.0 66-67 35.77090778635747 37.0 37.0 40.0 33.0 40.0 68-69 35.55457147813688 37.0 37.0 37.0 33.0 40.0 70-71 35.33326792360377 37.0 37.0 37.0 33.0 40.0 72-73 35.12473615771988 37.0 35.0 37.0 33.0 40.0 74-75 34.89453685460361 37.0 33.0 37.0 27.0 38.5 76-77 34.70281519265879 37.0 33.0 37.0 27.0 37.0 78-79 34.52635746429888 37.0 33.0 37.0 27.0 37.0 80-81 34.36842104362124 37.0 33.0 37.0 27.0 37.0 82-83 34.254854865346374 37.0 33.0 37.0 27.0 37.0 84-85 34.153936358286046 37.0 33.0 37.0 27.0 37.0 86-87 34.047802351021986 37.0 33.0 37.0 27.0 37.0 88-89 33.96070871911188 37.0 33.0 37.0 27.0 37.0 90-91 33.87364898410117 37.0 33.0 37.0 27.0 37.0 92-93 33.79583614063827 37.0 33.0 37.0 27.0 37.0 94-95 33.716264652990475 37.0 33.0 37.0 27.0 37.0 96-97 33.62495572669284 37.0 33.0 37.0 27.0 37.0 98-99 33.55614931983646 37.0 33.0 37.0 27.0 37.0 100-101 31.775930785651 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14667252257805075 2306 2 0.27491828175514144 2306 3 0.32717818600528403 2306 4 0.4238197868903555 2306 5 0.3872019502651298 2306 6 0.44326902532785795 2306 7 0.4477965431237507 2306 8 0.44779294221388 2306 9 0.4630940443258922 2306 10-11 0.4595960064032454 2306 12-13 0.43035683024553606 2306 14-15 0.7768991554491009 2306 16-17 0.7907737140100508 2306 18-19 0.806355887122983 2306 20-21 0.7912582320974124 2306 22-23 0.7926622056679875 2306 24-25 0.8180647513526011 2306 26-27 0.8387392601121988 2306 28-29 0.8661473854950259 2306 30-31 0.836746791816104 2306 32-33 0.8808201854585676 2306 34-35 0.8589792544772479 2306 36-37 0.8771875878004991 2306 38-39 0.841936488713003 2306 40-41 0.8965737077487574 2306 42-43 0.8518343915634006 2306 44-45 0.9043587789060794 2306 46-47 0.9231548583503795 2306 48-49 0.9021705877450898 2306 50-51 0.8481594609499936 2306 52-53 0.8859543146586546 2306 54-55 0.9602099255332703 2306 56-57 0.9497478257304763 2306 58-59 0.9824022251985909 2306 60-61 0.9250638139653447 2306 62-63 0.9177902953230728 2306 64-65 0.9237868606927933 2306 66-67 0.9254454390026723 2306 68-69 0.9333718618242344 2306 70-71 0.9511518626769657 2306 72-73 0.9712947326870705 2306 74-75 0.9851732507627204 2306 76-77 0.9454504209194141 2306 78-79 0.9169559641545604 2306 80-81 0.9661136987388943 2306 82-83 0.9878269007091731 2306 84-85 0.9768882763463935 2306 86-87 1.0012802145081565 2306 88-89 0.9798613473415045 2306 90-91 0.9884480216926121 2306 92-93 0.9945455653680355 2306 94-95 0.9971389904117984 2306 96-97 0.9576228294791775 2306 98-99 0.9591100286641279 2306 100-101 0.8346729961338504 2307 1 0.2072018244079601 2307 2 0.2798311198703658 2307 3 0.27644188067751685 2307 4 0.389823072582999 2307 5 0.39408336174085434 2307 6 0.3725263949672595 2307 7 0.3660596500626738 2307 8 0.3614954393904668 2307 9 0.35010320939019834 2307 10-11 0.37386044907118077 2307 12-13 0.36971822029126855 2307 14-15 0.643703421831546 2307 16-17 0.6409165898135925 2307 18-19 0.6382432535540019 2307 20-21 0.6756398094023766 2307 22-23 0.6702192219146994 2307 24-25 0.6917445075781288 2307 26-27 0.7017491497287338 2307 28-29 0.6994220957760646 2307 30-31 0.7205333793123145 2307 32-33 0.732401802374369 2307 34-35 0.7450211568200586 2307 36-37 0.7148802947012811 2307 38-39 0.681716344718339 2307 40-41 0.7632117371830986 2307 42-43 0.7558085401576804 2307 44-45 0.7823441853845097 2307 46-47 0.7546291178895146 2307 48-49 0.7355952225522628 2307 50-51 0.6387553124435215 2307 52-53 0.7433939708503488 2307 54-55 0.85214269193186 2307 56-57 0.8141498596201799 2307 58-59 0.8036585400386045 2307 60-61 0.7991051132072258 2307 62-63 0.8306439839925233 2307 64-65 0.8301333827166459 2307 66-67 0.7803902394289466 2307 68-69 0.8091019456154314 2307 70-71 0.7812892308329182 2307 72-73 0.7291544876486853 2307 74-75 0.7915461427712742 2307 76-77 0.7271808748665549 2307 78-79 0.8041320816240685 2307 80-81 0.7891016961521089 2307 82-83 0.7848161611688056 2307 84-85 0.8076885576523694 2307 86-87 0.7830998418526249 2307 88-89 0.7345317337055874 2307 90-91 0.7422041930491687 2307 92-93 0.7702774975867683 2307 94-95 0.7682061109513825 2307 96-97 0.7950903575274495 2307 98-99 0.7490963459880504 2307 100-101 0.7476355087690187 2201 1 0.24868158260817097 2201 2 0.2852181478013094 2201 3 0.30542367949812643 2201 4 0.4051696645667491 2201 5 0.39684081106366875 2201 6 0.4233830881706808 2201 7 0.3820057453560892 2201 8 0.3340072452497793 2201 9 0.4082080288090282 2201 10-11 0.3768298185280443 2201 12-13 0.4200620678898659 2201 14-15 0.6776128369689189 2201 16-17 0.680129725188273 2201 18-19 0.6469224730654233 2201 20-21 0.7223381503228836 2201 22-23 0.7166922776434248 2201 24-25 0.7373307602377608 2201 26-27 0.7251248718704417 2201 28-29 0.7175063693925239 2201 30-31 0.749762017104338 2201 32-33 0.7528935024985799 2201 34-35 0.799955537885289 2201 36-37 0.7780140144830412 2201 38-39 0.7264398181750948 2201 40-41 0.7500280401324275 2201 42-43 0.7567716605054073 2201 44-45 0.7909860684525611 2201 46-47 0.7691579572700036 2201 48-49 0.7861501960786086 2201 50-51 0.7222461581136983 2201 52-53 0.8134784040777845 2201 54-55 0.8569668550045932 2201 56-57 0.8449925677951242 2201 58-59 0.8100494115082384 2201 60-61 0.8588596688200951 2201 62-63 0.8571786092534452 2201 64-65 0.8828076800382405 2201 66-67 0.8654819216257934 2201 68-69 0.797237052634209 2201 70-71 0.8317782432510157 2201 72-73 0.8496417741550175 2201 74-75 0.8856813927471876 2201 76-77 0.8991436060182068 2201 78-79 0.9394028713387357 2201 80-81 0.9298563597350622 2201 82-83 0.9041394193711554 2201 84-85 0.9254200322303845 2201 86-87 0.957422294652801 2201 88-89 0.906367173178424 2201 90-91 0.9127151832900964 2201 92-93 0.9832132588216211 2201 94-95 0.9250111102806855 2201 96-97 0.948010157314684 2201 98-99 0.9064901548314808 2201 100-101 0.8011427290840167 1101 1 0.25124402246966326 1101 2 0.2708230514771941 1101 3 0.28240188942883293 1101 4 0.382138522504718 1101 5 0.3556907948566206 1101 6 0.37732338952520905 1101 7 0.3600878716418876 1101 8 0.3672594451784903 1101 9 0.3883187566489781 1101 10-11 0.39198075692397083 1101 12-13 0.3778533873517631 1101 14-15 0.6172899106306602 1101 16-17 0.6427567396680658 1101 18-19 0.628073215051046 1101 20-21 0.6367573300265832 1101 22-23 0.6580705501076594 1101 24-25 0.6693194905761786 1101 26-27 0.6176553359714134 1101 28-29 0.6264773861713024 1101 30-31 0.6731099165711427 1101 32-33 0.7104715860734743 1101 34-35 0.7284302415811439 1101 36-37 0.6776681592228968 1101 38-39 0.6216999051477643 1101 40-41 0.7021677769930506 1101 42-43 0.6966489135409546 1101 44-45 0.6688397235882348 1101 46-47 0.7161478988175318 1101 48-49 0.6891566628370924 1101 50-51 0.6425827804791382 1101 52-53 0.7064544835618278 1101 54-55 0.7339418947265912 1101 56-57 0.7012440373829989 1101 58-59 0.7287882081007027 1101 60-61 0.7576625408890081 1101 62-63 0.7407175396808512 1101 64-65 0.7426196257289988 1101 66-67 0.7887195585772986 1101 68-69 0.7271175603599787 1101 70-71 0.7226542562160176 1101 72-73 0.7476924763925226 1101 74-75 0.7302572975229751 1101 76-77 0.7965611780399584 1101 78-79 0.8320898399006822 1101 80-81 0.8172569760590562 1101 82-83 0.8018594493486901 1101 84-85 0.7664912500591754 1101 86-87 0.7493470874889496 1101 88-89 0.8161880868829954 1101 90-91 0.7576017682125098 1101 92-93 0.7826409184032315 1101 94-95 0.7362570109751658 1101 96-97 0.8345347437035926 1101 98-99 0.8390254479670176 1101 100-101 0.7877295996830718 2304 1 0.1171627993251434 2304 2 0.2367641706029744 2304 3 0.2597413025383801 2304 4 0.3466083018703401 2304 5 0.34625739904377895 2304 6 0.3525400763334048 2304 7 0.28784392515769497 2304 8 0.35099181945218305 2304 9 0.32325708696041033 2304 10-11 0.37899346901149755 2304 12-13 0.37437710345295727 2304 14-15 0.626735845009847 2304 16-17 0.618079854423577 2304 18-19 0.5908624976308587 2304 20-21 0.6299897186388606 2304 22-23 0.6675469487151489 2304 24-25 0.6434319621157059 2304 26-27 0.6821650300702586 2304 28-29 0.6959165222788286 2304 30-31 0.7469138282344474 2304 32-33 0.7487064414084941 2304 34-35 0.7433378970409734 2304 36-37 0.7648598832830587 2304 38-39 0.7077502697123776 2304 40-41 0.7646400294001126 2304 42-43 0.7506464058276876 2304 44-45 0.7560521859413498 2304 46-47 0.7487564099101647 2304 48-49 0.7680214616424337 2304 50-51 0.6787821496785895 2304 52-53 0.7908053531200707 2304 54-55 0.8365526092628457 2304 56-57 0.7808187419797861 2304 58-59 0.7709188003717671 2304 60-61 0.7711395895123658 2304 62-63 0.7954804483366118 2304 64-65 0.7611790207670666 2304 66-67 0.7762564293989627 2304 68-69 0.8097664414561692 2304 70-71 0.8105484364839555 2304 72-73 0.8344848732806724 2304 74-75 0.862031536253447 2304 76-77 0.85094652339005 2304 78-79 0.8258900818582404 2304 80-81 0.8621536272621881 2304 82-83 0.8585958690476687 2304 84-85 0.8946526772555359 2304 86-87 0.9467765614961721 2304 88-89 0.9254705759706852 2304 90-91 0.9170936865159476 2304 92-93 0.9293240520305872 2304 94-95 0.9434519284084075 2304 96-97 0.923191367311837 2304 98-99 0.9395084833142988 2304 100-101 1.0560224720127955 2202 1 0.19995910298298014 2202 2 0.20783558288557202 2202 3 0.2147665175504443 2202 4 0.26403588282482815 2202 5 0.26006281479502746 2202 6 0.2804630301180211 2202 7 0.2700795297567993 2202 8 0.2787523215869996 2202 9 0.2126030830370027 2202 10-11 0.2445994337330859 2202 12-13 0.2587786079854695 2202 14-15 0.4247743911201951 2202 16-17 0.4167420634217649 2202 18-19 0.388243725584168 2202 20-21 0.4394453104860361 2202 22-23 0.4080845367118897 2202 24-25 0.4229374803081001 2202 26-27 0.4025020718992707 2202 28-29 0.4573499012252853 2202 30-31 0.46521372949487017 2202 32-33 0.49948698242837253 2202 34-35 0.4939192614767265 2202 36-37 0.44630102232184043 2202 38-39 0.5116760515020147 2202 40-41 0.49435945777611323 2202 42-43 0.459953017122281 2202 44-45 0.48293064848594724 2202 46-47 0.4968477312582351 2202 48-49 0.4197787814784064 2202 50-51 0.4443086719423306 2202 52-53 0.5514939297750416 2202 54-55 0.6104447952729259 2202 56-57 0.5749779399431745 2202 58-59 0.5196505977003696 2202 60-61 0.5452429002691659 2202 62-63 0.5319831783159401 2202 64-65 0.5571306321627958 2202 66-67 0.4995408719473744 2202 68-69 0.5238395433765888 2202 70-71 0.4413602174938873 2202 72-73 0.5373608930486853 2202 74-75 0.506800327145136 2202 76-77 0.5180618154914285 2202 78-79 0.5319980174714161 2202 80-81 0.527108558576117 2202 82-83 0.5543179020120661 2202 84-85 0.5631448318286374 2202 86-87 0.505158592823939 2202 88-89 0.5958227374207965 2202 90-91 0.5149127119091688 2202 92-93 0.5406114870398113 2202 94-95 0.5739767552175508 2202 96-97 0.5338734979344224 2202 98-99 0.5352066252415 2202 100-101 0.5984789986404202 1102 1 0.1252168309517927 1102 2 0.14804612472688206 1102 3 0.17852756021942895 1102 4 0.18142810251124075 1102 5 0.16304599134090836 1102 6 0.210899842165567 1102 7 0.2170276855193407 1102 8 0.23009033712715166 1102 9 0.22017888411228626 1102 10-11 0.20667086554107783 1102 12-13 0.21542817640529677 1102 14-15 0.34936652545061264 1102 16-17 0.33950119286888736 1102 18-19 0.28885446296609274 1102 20-21 0.3319063854752997 1102 22-23 0.32150004860045556 1102 24-25 0.38906890554778784 1102 26-27 0.3669781962727825 1102 28-29 0.4016000358013301 1102 30-31 0.30068828720855123 1102 32-33 0.43478171953541533 1102 34-35 0.3505961016410737 1102 36-37 0.4395703345267279 1102 38-39 0.40942448415616184 1102 40-41 0.39156746276881904 1102 42-43 0.4067081570342026 1102 44-45 0.42033583975811695 1102 46-47 0.37423438486499094 1102 48-49 0.40396508317658686 1102 50-51 0.4326075926478907 1102 52-53 0.4089812220990794 1102 54-55 0.516058863086819 1102 56-57 0.5274979645078162 1102 58-59 0.4720325915153083 1102 60-61 0.48592737915789286 1102 62-63 0.46824584794098456 1102 64-65 0.4635005309472362 1102 66-67 0.4802378597621413 1102 68-69 0.4002997769625054 1102 70-71 0.4835336689167846 1102 72-73 0.5065227187790668 1102 74-75 0.48051742432090094 1102 76-77 0.47689075608024467 1102 78-79 0.4710122485558017 1102 80-81 0.5005711948747233 1102 82-83 0.5143072716575787 1102 84-85 0.45809994365641415 1102 86-87 0.4228518599949638 1102 88-89 0.4660919043658751 1102 90-91 0.4668553560312816 1102 92-93 0.41830449435816064 1102 94-95 0.49447149287144043 1102 96-97 0.49290743100516465 1102 98-99 0.5406709768037956 1102 100-101 0.5892513587784798 2305 1 0.15063133710287957 2305 2 0.1473329769370153 2305 3 0.19249489546226073 2305 4 0.14965312265751862 2305 5 0.2099253068359559 2305 6 0.23121495527726665 2305 7 0.2179706583344867 2305 8 0.22056144884042794 2305 9 0.16730326132392292 2305 10-11 0.21322727928026808 2305 12-13 0.22622819137052375 2305 14-15 0.35273094582356634 2305 16-17 0.3038741800083642 2305 18-19 0.295165437382515 2305 20-21 0.34232650701545 2305 22-23 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39477.0 12 31596.0 13 31952.0 14 35525.0 15 39701.0 16 45083.0 17 50873.0 18 57358.0 19 64516.0 20 72685.0 21 81109.0 22 91319.0 23 102801.0 24 117706.0 25 135208.0 26 157646.0 27 185614.0 28 222154.0 29 268539.0 30 333337.0 31 422560.0 32 553844.0 33 738660.0 34 1022584.0 35 1583897.0 36 3279994.0 37 8400597.0 38 8036377.0 39 6686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.005459746861955 13.251082667326724 10.411557336862098 57.33190024894923 2 22.760799358461377 19.856627038586556 39.87607561038751 17.50649799256455 3 22.589815105301376 24.875936783191623 25.290580661738776 27.24366744976822 4 25.379744593725327 29.56657636690268 18.338716661734647 26.714962377637345 5 27.787854471714706 31.037513695496987 21.483358312356067 19.691273520432237 6 20.178093836643946 33.49981333687113 22.85645460689324 23.465638219591693 7 19.20248912396368 14.47092729822376 41.57860216185178 24.747981415960783 8 20.601976176147325 20.0686534537009 27.827045843410342 31.502324526741432 9 21.38210866526006 19.456866046472122 31.61165072949893 27.54937455876889 10-11 25.009373747604684 28.349569295003175 21.146129366665175 25.494927590726967 12-13 22.906552973524068 22.515602229525516 27.89056432801437 26.68728046893605 14-15 23.750803531563722 24.155441160804234 26.241858519817384 25.85189678781466 16-17 25.1814097535142 24.418763850679063 24.758858091866987 25.640968303939754 18-19 24.6189507414834 24.76355749761252 24.777272341682878 25.840219419221206 20-21 24.60633348429639 24.949301404748812 25.2356143688383 25.208750742116496 22-23 24.56945916570212 24.94433964589413 25.169021075295984 25.317180113107767 24-25 24.25788562665719 25.15206024355804 25.239337292373527 25.350716837411248 26-27 24.272949498442344 25.28692151677569 25.44510648382022 24.995022500961745 28-29 24.59081552644017 24.85732721097552 25.089255822616902 25.462601439967408 30-31 24.44659477745148 24.819253444734663 25.18776536760537 25.546386410208484 32-33 24.616917510634188 25.097613415662973 25.262411959873628 25.023057113829218 34-35 24.85822868823831 25.02006067085123 24.856977306933338 25.26473333397712 36-37 24.46968440476647 25.103559741677355 24.939974647805087 25.48678120575109 38-39 24.587405920440634 25.12809598750364 25.312600907079357 24.97189718497637 40-41 24.85396807078365 24.921657352595837 25.013231814396796 25.21114276222372 42-43 24.478976083938615 24.795698807182294 25.31939336130325 25.405931747575845 44-45 24.82716305151073 24.993925572059894 25.22594224671216 24.952969129717214 46-47 24.978416539874612 24.82694801406737 24.940601315780075 25.25403413027794 48-49 24.562207209265015 24.785150423945876 25.026403150417163 25.626239216371943 50-51 24.711601939905943 25.007890333242884 25.172206570638345 25.108301156212832 52-53 24.94014084795314 24.805292946717564 24.98538970036277 25.269176504966527 54-55 24.439116131495272 24.84677376929835 25.16062224623608 25.553487852970296 56-57 24.591519298678744 25.02272381118961 25.434194399979333 24.95156249015232 58-59 24.807643854487974 24.984704671205694 24.942034069194158 25.265617405112174 60-61 24.457994457397934 25.093848974441922 25.069169237440867 25.378987330719273 62-63 24.69517418388248 25.242675250630285 25.12727820122246 24.934872364264777 64-65 24.759609310299265 25.204049379242356 24.85318325752999 25.18315805292839 66-67 24.57348385598361 25.112706614829793 24.994109864859077 25.319699664327516 68-69 24.75303112553386 25.295394106633157 25.079170965509235 24.872403802323753 70-71 24.908457962525404 25.072605260299664 24.848216351717628 25.170720425457304 72-73 24.662837616987616 25.089543302026197 24.96040893735703 25.287210143629157 74-75 24.78372410067509 25.282087942795524 25.048709483925002 24.88547847260438 76-77 24.920546031062347 25.131674551574562 24.81518467091755 25.13259474644554 78-79 24.72350879261234 25.07596048372604 24.949350310556618 25.251180413105008 80-81 24.873134093131146 25.311563225547122 24.898198069597672 24.917104611724056 82-83 25.063120978420816 25.144640559315228 24.694985170009485 25.097253292254468 84-85 24.711871734592027 25.146994773519165 24.96837992804677 25.172753563842036 86-87 24.925316932776184 25.3900280777901 24.952966430835623 24.73168855859809 88-89 25.03918089664782 25.16606035141579 24.65461587334571 25.140142878590677 90-91 24.936620967821334 25.037849931546287 24.79547630164182 25.23005279899056 92-93 24.955054985096016 25.32101297121813 24.88161470887878 24.842317334807078 94-95 25.211095404387745 25.142323007756833 24.690168157734888 24.95641343012053 96-97 24.90356018122504 25.186193766732885 24.820342153973268 25.089903898068812 98-99 25.107477102683518 25.31483375835885 24.849404113544345 24.728285025413285 100-101 25.42127159109608 25.14637655269885 24.471391506140495 24.960960350064575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18086.0 1 9797.0 2 1130.5 3 674.5 4 593.0 5 581.5 6 554.5 7 554.5 8 561.5 9 574.0 10 600.0 11 638.0 12 689.5 13 742.5 14 807.0 15 876.5 16 1037.5 17 1217.5 18 1407.5 19 1817.5 20 2450.5 21 3286.0 22 4545.5 23 6605.0 24 9787.0 25 14463.0 26 21258.0 27 30697.5 28 43239.5 29 58878.0 30 79005.5 31 105229.0 32 136982.5 33 177379.0 34 229130.5 35 293194.0 36 371280.0 37 467300.5 38 579146.5 39 697520.5 40 816293.0 41 928506.5 42 1026794.5 43 1094148.0 44 1127134.0 45 1128041.5 46 1098718.0 47 1048506.5 48 989845.0 49 928652.0 50 863635.0 51 806239.0 52 763087.0 53 732675.0 54 716588.0 55 706536.0 56 693598.5 57 685738.0 58 668183.0 59 635883.5 60 603856.0 61 569782.5 62 545246.0 63 516166.0 64 458849.0 65 411510.0 66 386958.0 67 354678.0 68 314789.0 69 270162.0 70 226414.5 71 187865.0 72 155929.5 73 128944.5 74 97381.5 75 70424.5 76 51305.5 77 35930.5 78 24692.5 79 17189.0 80 11412.5 81 7190.0 82 4708.0 83 2987.0 84 1834.0 85 1253.5 86 924.5 87 704.0 88 549.5 89 435.0 90 358.5 91 285.0 92 220.0 93 166.0 94 135.0 95 103.5 96 72.0 97 45.0 98 24.5 99 16.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09592708400306023 2 0.08551833913562526 3 0.09045640596235428 4 0.10880486228385972 5 0.09214554438382398 6 0.09274282981664099 7 0.10162602399261345 8 0.08847052700738313 9 0.11765001280169096 10-11 0.0890792255376425 12-13 0.08291805510167358 14-15 0.13340198945505885 16-17 0.13342671783285062 18-19 0.12559352862157555 20-21 0.13825826241678424 22-23 0.13603270841552348 24-25 0.11022579291543388 26-27 0.11388178846280415 28-29 0.10301842188058172 30-31 0.09139798650134921 32-33 0.1125844997201889 34-35 0.13162535061986433 36-37 0.11815789563787611 38-39 0.12001823052098126 40-41 0.14909119407249372 42-43 0.12364379114183856 44-45 0.12207829460932776 46-47 0.14039060945559906 48-49 0.1509153874803647 50-51 0.12153427029790848 52-53 0.09187733659392847 54-55 0.09445669661590247 56-57 0.09982846114544128 58-59 0.09557137579943992 60-61 0.09579583338247306 62-63 0.08504089122595308 64-65 0.1075931717720622 66-67 0.10539424833150025 68-69 0.10297847603953346 70-71 0.10333418424315376 72-73 0.10084232463490451 74-75 0.13922076696775684 76-77 0.15654584888526374 78-79 0.15544068061626162 80-81 0.12467857864327946 82-83 0.1045496791207654 84-85 0.10855377414012773 86-87 0.11466548782051306 88-89 0.1109809595295369 90-91 0.11511440298657934 92-93 0.10210156971937857 94-95 0.09808035505385271 96-97 0.10217195048693979 98-99 0.09067135263085212 100-101 0.09795861534780083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.628559E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.23787650494147 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29238027003085 38.808761858040214 2 12.982929598239881 13.564013460504084 3 5.062254097793612 7.933242131915296 4 2.4343016829461184 5.086510027580421 5 1.328653831441768 3.4703027382336193 6 0.849389186095793 2.662217244474287 7 0.636288870471514 2.326686560601175 8 0.47643900447483856 1.99105295023151 9 0.3362057009713946 1.5806404698840895 >10 1.4670317883657478 13.931365682965744 >50 0.08856879857701415 3.191728912291511 >100 0.04258956846054606 3.8646238146301535 >500 0.0022868994554516844 0.8319063664519977 >1k 6.571186986344883E-4 0.6468710728436954 >5k 1.604064562634998E-5 0.06617586552326338 >10k+ 7.543331252398348E-6 0.04390084382887364 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.008270691279898986 6.467421884005647E-5 7.608731628241938E-6 2 0.00899352078458197 6.467421884005647E-5 2.2826194884725814E-5 3 0.011629946293767802 7.608731628241938E-5 2.2826194884725814E-5 4 0.020714771857888677 7.608731628241938E-5 4.1848023955330656E-5 5 0.022327822963075967 7.608731628241938E-5 4.94567555835726E-5 6 0.02716697627863784 9.510914535302423E-5 6.08698530259355E-5 7 0.0281218720979822 1.103266086095081E-4 6.08698530259355E-5 8 0.030887646044848147 1.103266086095081E-4 6.08698530259355E-5 9 0.0343267927408135 1.1793534023775003E-4 6.08698530259355E-5 10-11 0.04152655504403743 1.2554407186599197E-4 6.277203593299599E-5 12-13 0.04956518000927504 1.3885935221541538E-4 7.228295046829841E-5 14-15 0.058140220554303707 1.5217463256483876E-4 8.179386500360083E-5 16-17 0.06717178499702689 1.6739209582132265E-4 8.369604791066131E-5 18-19 0.07644873103476088 1.7500082744956457E-4 8.369604791066131E-5 20-21 0.08456724768209502 1.84511741984867E-4 8.369604791066131E-5 22-23 0.0923471757719724 2.0543575396253234E-4 9.130477953890326E-5 24-25 0.0989230220816805 2.1875103431195572E-4 1.0462005988832665E-4 26-27 0.10445647215831944 2.3587068047550006E-4 1.0652224279538713E-4 28-29 0.10954861580052036 2.43479412103742E-4 1.122287915165686E-4 30-31 0.11396168014490068 2.6440342408140733E-4 1.464680838436573E-4 32-33 0.11781930708041935 2.7581652152377026E-4 1.7880519326368553E-4 34-35 0.1214848135423249 2.8152307024495167E-4 2.0163138814841136E-4 36-37 0.12473183976467714 3.0054489931555657E-4 2.073379368695928E-4 38-39 0.12809870351017422 3.1005581385085894E-4 2.1304448559077426E-4 40-41 0.13104898919902502 3.157623625720404E-4 2.206532172190162E-4 42-43 0.13353704444146014 3.3668637454970575E-4 2.548925095461049E-4 44-45 0.13610689354889885 3.6141475234149206E-4 2.7201215570964925E-4 46-47 0.13870527539994348 3.65219118155613E-4 2.7771870443083076E-4 48-49 0.14115718916714443 3.766322155979759E-4 2.872296189661332E-4 50-51 0.1435843745565536 3.9755622757564125E-4 2.9674053350143556E-4 52-53 0.14596971192200747 4.0706714211094367E-4 3.005448993155565E-4 54-55 0.14826945105664358 4.1277369083212513E-4 3.1576236257204046E-4 56-57 0.15051973343569613 4.184802395533066E-4 3.2337109420028237E-4 58-59 0.15274528743695692 4.2228460536742756E-4 3.252732771073429E-4 60-61 0.15529421253241793 4.3940425153097195E-4 3.309798258285243E-4 62-63 0.15805998647928388 4.660348122298187E-4 3.3668637454970575E-4 64-65 0.16097413069290056 4.8125227548630255E-4 3.461972890850082E-4 66-67 0.16390539455268077 4.90763190021605E-4 3.557082036203106E-4 68-69 0.1670382898006094 4.983719216498469E-4 3.5951256943443156E-4 70-71 0.17062961112913957 5.135893849063308E-4 3.65219118155613E-4 72-73 0.17522338284969063 5.192959336275122E-4 3.766322155979759E-4 74-75 0.1809166162905227 5.250024823486936E-4 3.8424094722621787E-4 76-77 0.18741066873522716 5.364155797910567E-4 3.937518617615203E-4 78-79 0.19524385794650223 5.421221285122381E-4 4.0706714211094367E-4 80-81 0.2054319495967182 5.573395917687219E-4 4.146758737391856E-4 82-83 0.21944152670721867 5.687526892110849E-4 4.717413609510001E-4 84-85 0.23932504463472193 5.744592379322663E-4 4.90763190021605E-4 86-87 0.26473820827305 5.915788840958107E-4 4.94567555835726E-4 88-89 0.2967386313185285 6.106007131664155E-4 4.964697387427864E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGATT 13400 0.0 12.5448475 1 CGAATTA 8265 0.0 12.216143 14-15 CTCGGTT 11870 0.0 12.161573 1 CTCGTTT 12090 0.0 11.547499 1 CTCGACT 13625 0.0 11.501231 1 CTCGGGT 13725 0.0 11.452032 1 CGGGAAT 7810 0.0 10.883509 1 TTTAAAA 16465 0.0 10.829246 56-57 CTCGGAT 13135 0.0 10.809561 1 CTCGAAT 11655 0.0 10.715449 1 CTCAGAT 20865 0.0 10.673858 1 CTTGGAT 31810 0.0 10.613848 1 CGCAAAT 6435 0.0 10.552478 1 CGACGAT 18135 0.0 10.552477 1 CTCGTAT 7125 0.0 10.530262 1 CCCGAAT 5615 0.0 9.9792795 1 CTCAAAT 21885 0.0 9.959397 1 CTTAAAT 11435 0.0 9.8834305 1 TCGGGGG 12595 0.0 9.770247 38-39 CTTTAAT 12790 0.0 9.764547 1 >>END_MODULE