FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 kx24-2.3420000000cdfb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 kx24-2.3420000000cdfb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26285590
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT134000.012.54484751
CGAATTA82650.012.21614314-15
CTCGGTT118700.012.1615731
CTCGTTT120900.011.5474991
CTCGACT136250.011.5012311
CTCGGGT137250.011.4520321
CGGGAAT78100.010.8835091
TTTAAAA164650.010.82924656-57
CTCGGAT131350.010.8095611
CTCGAAT116550.010.7154491
CTCAGAT208650.010.6738581
CTTGGAT318100.010.6138481
CGCAAAT64350.010.5524781
CGACGAT181350.010.5524771
CTCGTAT71250.010.5302621
CCCGAAT56150.09.97927951
CTCAAAT218850.09.9593971
CTTAAAT114350.09.88343051
TCGGGGG125950.09.77024738-39
CTTTAAT127900.09.7645471
Produced by FastQC (version 0.11.2)