##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 kx12-3.3420000000cd67.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21188867 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.427455842731 33.0 33.0 33.0 33.0 33.0 2 32.38630149502566 33.0 33.0 33.0 33.0 33.0 3 32.37477039239521 33.0 33.0 33.0 33.0 33.0 4 36.215441486323925 37.0 37.0 37.0 37.0 37.0 5 36.20196922279988 37.0 37.0 37.0 37.0 37.0 6 36.18552327502929 37.0 37.0 37.0 37.0 37.0 7 36.23914114898168 37.0 37.0 37.0 37.0 37.0 8 36.229309854085166 37.0 37.0 37.0 37.0 37.0 9 36.22352020992911 37.0 37.0 37.0 37.0 37.0 10-11 36.19849263294729 37.0 37.0 37.0 37.0 37.0 12-13 36.19583503450185 37.0 37.0 37.0 37.0 37.0 14-15 38.38057568155956 40.0 37.0 40.0 37.0 40.0 16-17 38.3631998586805 40.0 37.0 40.0 37.0 40.0 18-19 38.340010888737 40.0 37.0 40.0 37.0 40.0 20-21 38.30199559513966 40.0 37.0 40.0 37.0 40.0 22-23 38.26828046067777 40.0 37.0 40.0 37.0 40.0 24-25 38.228838332884905 40.0 37.0 40.0 37.0 40.0 26-27 38.1748157888763 40.0 37.0 40.0 37.0 40.0 28-29 38.12919973493628 40.0 37.0 40.0 37.0 40.0 30-31 38.07611343730649 40.0 37.0 40.0 37.0 40.0 32-33 37.98648561529977 40.0 37.0 40.0 33.0 40.0 34-35 37.90126458861628 40.0 37.0 40.0 33.0 40.0 36-37 37.82380060717735 40.0 37.0 40.0 33.0 40.0 38-39 37.718792444164194 40.0 37.0 40.0 33.0 40.0 40-41 37.59468684663507 40.0 37.0 40.0 33.0 40.0 42-43 37.48518259612466 40.0 37.0 40.0 33.0 40.0 44-45 37.35829721334322 40.0 37.0 40.0 33.0 40.0 46-47 37.225771793272386 40.0 37.0 40.0 33.0 40.0 48-49 37.06667760952013 40.0 37.0 40.0 33.0 40.0 50-51 35.61472557735154 38.5 35.0 38.5 30.0 38.5 52-53 35.94299088762037 37.0 35.0 38.5 33.0 40.0 54-55 36.63023065367299 37.0 37.0 40.0 33.0 40.0 56-57 36.588305547436775 37.0 37.0 40.0 33.0 40.0 58-59 36.45153924464201 37.0 37.0 40.0 33.0 40.0 60-61 36.274730428012035 37.0 37.0 40.0 33.0 40.0 62-63 36.09211070606088 37.0 37.0 40.0 33.0 40.0 64-65 35.89417178370132 37.0 37.0 40.0 33.0 40.0 66-67 35.69082353483081 37.0 37.0 38.5 33.0 40.0 68-69 35.48834697013295 37.0 37.0 37.0 33.0 40.0 70-71 35.27896805430889 37.0 35.0 37.0 33.0 40.0 72-73 35.08085656491213 37.0 33.0 37.0 33.0 40.0 74-75 34.862689991871676 37.0 33.0 37.0 27.0 37.0 76-77 34.68496748787936 37.0 33.0 37.0 27.0 37.0 78-79 34.516698698424975 37.0 33.0 37.0 27.0 37.0 80-81 34.364570743683466 37.0 33.0 37.0 27.0 37.0 82-83 34.25889687730826 37.0 33.0 37.0 27.0 37.0 84-85 34.161633394555736 37.0 33.0 37.0 27.0 37.0 86-87 34.057494744763844 37.0 33.0 37.0 27.0 37.0 88-89 33.97253284000509 37.0 33.0 37.0 27.0 37.0 90-91 33.88688722714622 37.0 33.0 37.0 27.0 37.0 92-93 33.80537135373967 37.0 33.0 37.0 27.0 37.0 94-95 33.72665968878846 37.0 33.0 37.0 27.0 37.0 96-97 33.632191518310066 37.0 33.0 37.0 27.0 37.0 98-99 33.56256981555455 37.0 33.0 37.0 27.0 37.0 100-101 31.737541016232722 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18174273274244968 2306 2 0.27993620503500694 2306 3 0.32778909383477384 2306 4 0.4267600259829649 2306 5 0.40692094887906194 2306 6 0.44314543807224993 2306 7 0.41810270099414026 2306 8 0.4526582118688012 2306 9 0.4460524109551969 2306 10-11 0.4533694573550022 2306 12-13 0.426309651869623 2306 14-15 0.7609707600526932 2306 16-17 0.7692729360001564 2306 18-19 0.7895849421544483 2306 20-21 0.8156911060219443 2306 22-23 0.7662991623613351 2306 24-25 0.8015740524818682 2306 26-27 0.8223379180443189 2306 28-29 0.8303255399726766 2306 30-31 0.8323669058013792 2306 32-33 0.8936390535932333 2306 34-35 0.8579933325308744 2306 36-37 0.8749047673009471 2306 38-39 0.7973922125631034 2306 40-41 0.8876707226920004 2306 42-43 0.8457979030414222 2306 44-45 0.8658564908780164 2306 46-47 0.8875705423734033 2306 48-49 0.9031791844775725 2306 50-51 0.807419799078481 2306 52-53 0.8756710279765088 2306 54-55 0.9492647647084027 2306 56-57 0.926313058698085 2306 58-59 0.9406056699832916 2306 60-61 0.9018203110369427 2306 62-63 0.8964501735572057 2306 64-65 0.9067340613187724 2306 66-67 0.8690739576337023 2306 68-69 0.9539292296363726 2306 70-71 0.9414259089975232 2306 72-73 0.9499720473086555 2306 74-75 0.9671127562725346 2306 76-77 0.9322634897777178 2306 78-79 0.9108537126583158 2306 80-81 0.956710570706143 2306 82-83 0.9922212969859174 2306 84-85 0.981318060064595 2306 86-87 1.0333432407343466 2306 88-89 1.0187614869037347 2306 90-91 1.0316378575494483 2306 92-93 1.088707762312552 2306 94-95 1.011871205496341 2306 96-97 0.9410656076348616 2306 98-99 0.9652651543963131 2306 100-101 0.8572927377735375 2307 1 0.2025962805678816 2307 2 0.2406758868647998 2307 3 0.26533135536005403 2307 4 0.3605887668032892 2307 5 0.38560488391730274 2307 6 0.37547321147503254 2307 7 0.39343356462202195 2307 8 0.37298121155632913 2307 9 0.3695072365732628 2307 10-11 0.3839118916306674 2307 12-13 0.3724681184808176 2307 14-15 0.6640035855389641 2307 16-17 0.6114879671712856 2307 18-19 0.6288721435518383 2307 20-21 0.6511340311009093 2307 22-23 0.6521923929861302 2307 24-25 0.6918677427107056 2307 26-27 0.7034223493027554 2307 28-29 0.6738024145207646 2307 30-31 0.7039330594090742 2307 32-33 0.6862798423759671 2307 34-35 0.7231862073747735 2307 36-37 0.7357896859680793 2307 38-39 0.7016445063646657 2307 40-41 0.7445087586786698 2307 42-43 0.7050654179410643 2307 44-45 0.7531136163065995 2307 46-47 0.7337878979817702 2307 48-49 0.7277860383457266 2307 50-51 0.6603177477173148 2307 52-53 0.6966127166962224 2307 54-55 0.8191414148407432 2307 56-57 0.8172670847464332 2307 58-59 0.7913663960418447 2307 60-61 0.7701593912628795 2307 62-63 0.7998648095932666 2307 64-65 0.7899349338954025 2307 66-67 0.8097805766834512 2307 68-69 0.813619203847253 2307 70-71 0.7743013824661347 2307 72-73 0.7693223854413489 2307 74-75 0.7722941694679477 2307 76-77 0.7276238196812912 2307 78-79 0.7999186355316041 2307 80-81 0.7922523818722524 2307 82-83 0.7813611544201109 2307 84-85 0.8216762077715032 2307 86-87 0.8501114331862922 2307 88-89 0.828807786439441 2307 90-91 0.7840655472384341 2307 92-93 0.812501988947453 2307 94-95 0.790047073147953 2307 96-97 0.8185025197612461 2307 98-99 0.7746393130643483 2307 100-101 0.7379241698012216 2201 1 0.24224715022931775 2201 2 0.27958034471402726 2201 3 0.306897271306255 2201 4 0.4045813912303018 2201 5 0.3978451024110825 2201 6 0.4216977995155631 2201 7 0.37084995547466093 2201 8 0.3490459279822531 2201 9 0.40009920341748284 2201 10-11 0.38742696260074894 2201 12-13 0.4192546249869409 2201 14-15 0.6871149948855546 2201 16-17 0.6898626900107985 2201 18-19 0.6342538759622016 2201 20-21 0.6982955566080733 2201 22-23 0.7066933754016631 2201 24-25 0.7324132750756291 2201 26-27 0.7176763378296798 2201 28-29 0.6884359669381865 2201 30-31 0.7301100380070054 2201 32-33 0.7575601920783299 2201 34-35 0.7897622805412894 2201 36-37 0.7760158365561836 2201 38-39 0.7536412012238358 2201 40-41 0.739969396008469 2201 42-43 0.7405371086364383 2201 44-45 0.7876624125146137 2201 46-47 0.7786216672161785 2201 48-49 0.8071599223201673 2201 50-51 0.7004074155182636 2201 52-53 0.7831720611697577 2201 54-55 0.8507569102325263 2201 56-57 0.8401660654740368 2201 58-59 0.8023774382469284 2201 60-61 0.824923671033325 2201 62-63 0.8550590067406318 2201 64-65 0.8686649164037519 2201 66-67 0.8683997587728953 2201 68-69 0.8508066364881017 2201 70-71 0.8618802015430589 2201 72-73 0.8721348816377414 2201 74-75 0.8677525192844016 2201 76-77 0.8908858732044749 2201 78-79 0.8928568028307424 2201 80-81 0.9011632787847503 2201 82-83 0.8715987386862523 2201 84-85 0.8839010232217674 2201 86-87 0.8884470167016829 2201 88-89 0.9226214452720214 2201 90-91 0.887688982955531 2201 92-93 0.9471084901828704 2201 94-95 0.9158151205782943 2201 96-97 0.9459026297018767 2201 98-99 0.973595066626082 2201 100-101 0.9043518238912505 1101 1 0.2551471370108658 1101 2 0.27019693265948064 1101 3 0.2513113495690078 1101 4 0.3450048147946063 1101 5 0.3368837611093767 1101 6 0.37990326234198335 1101 7 0.3391853815799166 1101 8 0.3530367844826401 1101 9 0.3610370043089972 1101 10-11 0.3768110860285816 1101 12-13 0.3332306256877331 1101 14-15 0.6112438869997305 1101 16-17 0.6351124408133231 1101 18-19 0.605723279650455 1101 20-21 0.6177460873845106 1101 22-23 0.6442580448706607 1101 24-25 0.6576294910339939 1101 26-27 0.6214110127869219 1101 28-29 0.6477045202021756 1101 30-31 0.6980150699656917 1101 32-33 0.7117917626477279 1101 34-35 0.6966144282595863 1101 36-37 0.6909338508282588 1101 38-39 0.626423811241942 1101 40-41 0.6774564833682746 1101 42-43 0.7103026921617115 1101 44-45 0.6636213098231636 1101 46-47 0.6945985705813129 1101 48-49 0.6969649719489794 1101 50-51 0.6577300013271739 1101 52-53 0.7314602406335382 1101 54-55 0.7327054821347758 1101 56-57 0.7213452717994073 1101 58-59 0.7479492949905264 1101 60-61 0.7864573393630252 1101 62-63 0.779893636711499 1101 64-65 0.7557291029091999 1101 66-67 0.7780676660405561 1101 68-69 0.7279574361256778 1101 70-71 0.758468894003677 1101 72-73 0.7348723624748246 1101 74-75 0.7342278012912331 1101 76-77 0.7723177119351519 1101 78-79 0.7817998919977427 1101 80-81 0.7630586143923992 1101 82-83 0.7900618204008225 1101 84-85 0.740056029845725 1101 86-87 0.717696896502872 1101 88-89 0.7365874992843331 1101 90-91 0.6731091058560068 1101 92-93 0.7332253814881469 1101 94-95 0.7315278368746405 1101 96-97 0.773424926089028 1101 98-99 0.7717679854129003 1101 100-101 0.7705151136687753 2304 1 0.14329771165273542 2304 2 0.2604349605624563 2304 3 0.26559511088782983 2304 4 0.36321187788572473 2304 5 0.38343606640789574 2304 6 0.3844254689918216 2304 7 0.32516564349514 2304 8 0.3689823268207988 2304 9 0.32797200763276635 2304 10-11 0.3957898692049824 2304 12-13 0.39087597673949404 2304 14-15 0.6649478825165502 2304 16-17 0.6554768241834097 2304 18-19 0.6288382007200326 2304 20-21 0.6641869915753844 2304 22-23 0.6941290596069152 2304 24-25 0.6982571880104587 2304 26-27 0.7339100083507049 2304 28-29 0.7345314827140044 2304 30-31 0.7707163720280974 2304 32-33 0.7918006121221381 2304 34-35 0.7933068328433635 2304 36-37 0.7698522217318526 2304 38-39 0.7488136598094783 2304 40-41 0.7795699215247467 2304 42-43 0.757888549282363 2304 44-45 0.7727612097865375 2304 46-47 0.7794571511798907 2304 48-49 0.8124542132630808 2304 50-51 0.7237628383037844 2304 52-53 0.8172450940484666 2304 54-55 0.8762911785456211 2304 56-57 0.8259859236550255 2304 58-59 0.817590536463257 2304 60-61 0.8231181655157229 2304 62-63 0.8453847564517716 2304 64-65 0.8391732286009557 2304 66-67 0.859383273987973 2304 68-69 0.8648258283702859 2304 70-71 0.8852739832863108 2304 72-73 0.910866963043155 2304 74-75 0.8985315469773738 2304 76-77 0.9188167951588255 2304 78-79 0.930733415539116 2304 80-81 0.9436563504714357 2304 82-83 0.9530376231410358 2304 84-85 0.969977062896632 2304 86-87 1.0318151476046253 2304 88-89 0.9970671345357189 2304 90-91 1.0512208492546407 2304 92-93 1.0003032495579305 2304 94-95 1.0451742899364262 2304 96-97 1.0612201129350396 2304 98-99 1.0614633046879547 2304 100-101 1.1148786872284653 2202 1 0.1922684256011209 2202 2 0.2136158994210433 2202 3 0.21507267649582218 2202 4 0.26320930816144994 2202 5 0.29786383623905266 2202 6 0.29257861836086363 2202 7 0.26687106292648366 2202 8 0.2795539721884239 2202 9 0.2264107414192722 2202 10-11 0.26063972569532723 2202 12-13 0.26544311707814217 2202 14-15 0.46168295335383647 2202 16-17 0.42750282654309046 2202 18-19 0.40630697734194854 2202 20-21 0.4721027040902044 2202 22-23 0.42730281873146936 2202 24-25 0.4409527883364035 2202 26-27 0.4380371196862356 2202 28-29 0.45270755629096016 2202 30-31 0.47044236573008646 2202 32-33 0.47724018027543735 2202 34-35 0.4996704564614376 2202 36-37 0.49040872159808657 2202 38-39 0.5204938415252727 2202 40-41 0.5159048477470165 2202 42-43 0.4828336828348938 2202 44-45 0.5044765766961063 2202 46-47 0.4710961130067446 2202 48-49 0.47252129326990655 2202 50-51 0.4518197908005064 2202 52-53 0.5484419869646189 2202 54-55 0.583330577293637 2202 56-57 0.5707488690472928 2202 58-59 0.5299118107230143 2202 60-61 0.539275949306905 2202 62-63 0.5317191063755828 2202 64-65 0.5734988930834675 2202 66-67 0.5082654004710889 2202 68-69 0.5707209799165653 2202 70-71 0.4619099651777674 2202 72-73 0.5816182045506295 2202 74-75 0.5669237721556541 2202 76-77 0.5789932944030411 2202 78-79 0.566966920790783 2202 80-81 0.6042209158707905 2202 82-83 0.6097053112730961 2202 84-85 0.5805619839133271 2202 86-87 0.5147210205318302 2202 88-89 0.588220320221005 2202 90-91 0.5755672521678292 2202 92-93 0.5753324074256483 2202 94-95 0.5612046720888131 2202 96-97 0.5274474682318768 2202 98-99 0.5708066615331262 2202 100-101 0.6333192988031904 1102 1 0.12895228357780297 1102 2 0.17868442018864528 1102 3 0.16469061466685275 1102 4 0.18642492832620405 1102 5 0.18152400031335247 1102 6 0.19305172050566455 1102 7 0.21721417705865775 1102 8 0.21513659190038936 1102 9 0.22668876758282863 1102 10-11 0.22280177703989779 1102 12-13 0.2204251141979796 1102 14-15 0.3451793076716356 1102 16-17 0.3577010309365747 1102 18-19 0.33788520041897385 1102 20-21 0.33919776047871153 1102 22-23 0.35942948766294336 1102 24-25 0.3775074428111722 1102 26-27 0.35635525383835187 1102 28-29 0.40624284066364424 1102 30-31 0.3305180924480098 1102 32-33 0.41898253858929024 1102 34-35 0.3678020215238291 1102 36-37 0.4608110117864044 1102 38-39 0.4245390966370266 1102 40-41 0.3977947746985464 1102 42-43 0.41556161679650216 1102 44-45 0.39679206290938396 1102 46-47 0.3840886083542898 1102 48-49 0.395253457112112 1102 50-51 0.43188202427514 1102 52-53 0.4387703301365491 1102 54-55 0.5097232527641538 1102 56-57 0.5569315985430308 1102 58-59 0.5271278587763533 1102 60-61 0.49519247723108606 1102 62-63 0.4514460402287952 1102 64-65 0.4647941233325099 1102 66-67 0.4694396671800689 1102 68-69 0.4209669408561467 1102 70-71 0.5058128378599633 1102 72-73 0.4989757583928949 1102 74-75 0.49351927411301233 1102 76-77 0.45158353084042346 1102 78-79 0.4715806840949597 1102 80-81 0.5119191826714342 1102 82-83 0.5530653383396924 1102 84-85 0.47635554572838856 1102 86-87 0.472608826568667 1102 88-89 0.4677025492892568 1102 90-91 0.4968769587488069 1102 92-93 0.43003053269050895 1102 94-95 0.508781702027747 1102 96-97 0.5151936368047245 1102 98-99 0.5285130670464468 1102 100-101 0.6064362432024133 2305 1 0.18988417409070024 2305 2 0.1964249061556984 2305 3 0.23957859486984034 2305 4 0.22417336208090433 2305 5 0.25085010581549483 2305 6 0.2564667590009435 2305 7 0.2563949396318179 2305 8 0.24469758671553876 2305 9 0.22760405925223637 2305 10-11 0.26427093453728645 2305 12-13 0.2847642412791558 2305 14-15 0.4158942708328013 2305 16-17 0.3719104685871386 2305 18-19 0.3691014466484219 2305 20-21 0.4199710718566223 2305 22-23 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Count 2 16601.0 3 1344.0 4 1128.0 5 965.0 6 787.0 7 1132.0 8 2042.0 9 4136.0 10 9844.0 11 14753.0 12 17573.0 13 21258.0 14 24947.0 15 28830.0 16 33553.0 17 38915.0 18 45069.0 19 51253.0 20 57813.0 21 65083.0 22 74374.0 23 84943.0 24 97305.0 25 111963.0 26 130571.0 27 154187.0 28 184671.0 29 223487.0 30 277650.0 31 354208.0 32 464316.0 33 619674.0 34 858328.0 35 1338151.0 36 2814017.0 37 6985462.0 38 5974206.0 39 4328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.166570959903876 12.324196691474842 9.916155962909237 56.59307638571205 2 21.937773516193104 18.4998996270851 40.882455050646996 18.679871806074804 3 24.25198611917811 23.330613122538725 24.134083025695695 28.28331773258747 4 27.70132665450071 28.387950510092185 17.293536375539574 26.61718645986752 5 28.8738791380829 29.7282108543525 21.431586527238103 19.9663234803265 6 20.81234848144521 32.303494892188205 23.33670470310571 23.54745192326088 7 19.140859511809627 14.405490757497622 41.534886924591135 24.91876280610161 8 21.15037859251227 19.347978853669513 27.896244882060966 31.605397671757252 9 22.157281648996964 19.034426457387703 31.020982421583938 27.787309472031392 10-11 25.424357293843958 27.605618733836394 21.11800521958881 25.85201875273084 12-13 23.27571629771126 22.132393179647387 27.628127696126892 26.963762826514458 14-15 24.17003072552642 23.67042696022524 25.895364126090133 26.264178188158212 16-17 25.416780082642443 23.943976761066562 24.513400570104306 26.125842586186693 18-19 24.977832362525092 24.181465449885263 24.478437368326123 26.362264819263526 20-21 24.936795978609876 24.36624428888293 24.91227571055375 25.784684021953446 22-23 24.954592790899195 24.39791778643391 24.6987240802853 25.94876534238159 24-25 24.78978044204893 24.438596763928228 24.71525472143874 26.056368072584103 26-27 24.727189631519256 24.55443563894999 24.99694543858777 25.721429290942982 28-29 25.02501909959965 24.21311220260979 24.63591709270256 26.125951605087998 30-31 24.85101604980319 24.248211951687622 24.70813987707921 26.192632121429988 32-33 24.990303750918773 24.45750023824259 24.799133264708757 25.753062746129874 34-35 25.2857979386582 24.3710904809488 24.40884080009695 25.93427078029605 36-37 24.982050558469727 24.4536491449106 24.426594160947623 26.13770613567205 38-39 25.113911350433515 24.560152781448874 24.73524872109608 25.590687147021523 40-41 25.31817874708022 24.3203573214569 24.528256183161417 25.833207748301458 42-43 24.93673115599408 24.222283518748764 24.752596652776507 26.08838867248065 44-45 25.116274410562138 24.426581534810172 24.822155867116944 25.63498818751075 46-47 25.417742310932972 24.29007242124623 24.435398854671465 25.856786413149337 48-49 25.117892120110852 24.213661219038425 24.53651558103446 26.13193107981627 50-51 25.152605750243545 24.472782671654684 24.7711451227799 25.603466455321872 52-53 25.455877247831676 24.295314066878387 24.4492039770655 25.79960470822444 54-55 25.056901203581738 24.226357621950587 24.591176272186296 26.12556490228138 56-57 25.084154283084594 24.47281027320596 24.898403209223005 25.544632234486446 58-59 25.3821487562157 24.372480485331216 24.40046259407115 25.844908164381934 60-61 25.038632834275255 24.373584756901362 24.582623081641366 26.005159327182014 62-63 25.185201157341762 24.54991064116338 24.732832158024465 25.532056043470387 64-65 25.337613569560823 24.502123243911715 24.425842086855162 25.734421099672293 66-67 25.136363683607154 24.361768449514116 24.517752365175745 25.984115501702988 68-69 25.240515384136053 24.567502688219413 24.68756491422146 25.504417013423076 70-71 25.50522573845109 24.414269172932364 24.35504573553102 25.725459353085515 72-73 25.248478680744142 24.375743227500323 24.50909427955993 25.86668381219561 74-75 25.294579719920797 24.579240945710904 24.661855286716445 25.464324047651854 76-77 25.502471058332095 24.475241168453614 24.36361205099106 25.658675722223233 78-79 25.353957509902557 24.40280434463507 24.443333203586153 25.799904941876218 80-81 25.407180091821115 24.662934752970422 24.483556141573935 25.44632901363453 82-83 25.618939353227866 24.53887559843932 24.209051455594576 25.63313359273824 84-85 25.302471100555096 24.489367193611223 24.45542634715845 25.75273535867523 86-87 25.442512083845493 24.735639930402794 24.51387213390566 25.307975851846052 88-89 25.55121920543946 24.504159440039864 24.21809923669009 25.72652211783059 90-91 25.471945075401287 24.374342789106468 24.304306441598143 25.849405693894102 92-93 25.48900761275073 24.64793809268004 24.48579811903269 25.377256175536537 94-95 25.72253542687643 24.532350917275984 24.188611430462256 25.55650222538533 96-97 25.4034043170355 24.548191369472168 24.336315022037287 25.712089291455055 98-99 25.574832398489338 24.68869585147217 24.41955232590148 25.316919424137012 100-101 25.95880952576661 24.496290184471807 23.99720990278073 25.547690386980843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14333.0 1 7764.0 2 887.5 3 534.0 4 467.0 5 453.0 6 440.5 7 415.0 8 418.5 9 415.5 10 409.5 11 458.0 12 514.0 13 527.5 14 565.5 15 637.5 16 725.0 17 853.0 18 1058.0 19 1301.0 20 1669.0 21 2246.5 22 3137.5 23 4579.0 24 6643.5 25 9593.5 26 13810.5 27 19518.0 28 27642.0 29 38166.0 30 50878.0 31 67450.5 32 88815.0 33 116142.5 34 151446.5 35 196743.0 36 253109.5 37 322882.5 38 405819.0 39 495148.0 40 584807.5 41 669449.0 42 745140.0 43 797509.0 44 828282.5 45 838242.0 46 825001.0 47 797860.5 48 762066.0 49 725734.5 50 690200.5 51 659024.0 52 637848.5 53 626775.5 54 624481.0 55 622359.5 56 618488.5 57 612617.0 58 593605.0 59 574365.0 60 555680.5 61 529236.5 62 504584.5 63 469127.0 64 417228.0 65 374699.0 66 347892.0 67 323129.0 68 294137.5 69 257502.5 70 219739.0 71 181807.5 72 147228.0 73 120246.0 74 92242.5 75 67599.0 76 49067.5 77 34235.0 78 23283.0 79 15734.5 80 10456.0 81 6466.5 82 3941.5 83 2387.0 84 1533.5 85 1075.5 86 766.5 87 562.0 88 446.5 89 356.0 90 307.0 91 259.5 92 182.0 93 129.0 94 99.0 95 71.5 96 52.5 97 33.5 98 16.0 99 10.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09457324924452072 2 0.08408661019959209 3 0.08938184377673426 4 0.10709869480043459 5 0.09071273136029406 6 0.09169909839917349 7 0.10016108931166542 8 0.08721561185881246 9 0.11510289813985805 10-11 0.08786217781252768 12-13 0.08161361341311926 14-15 0.13118917590072182 16-17 0.13132839995644882 18-19 0.12393300689461122 20-21 0.1360195427155213 22-23 0.1340586072865529 24-25 0.10864195806222202 26-27 0.11231841702531806 28-29 0.1016500787890169 30-31 0.09031629676093583 32-33 0.1107468370064336 34-35 0.12957984020570804 36-37 0.11590756598736497 38-39 0.11817998574440058 40-41 0.14735804420311854 42-43 0.12178329308499601 44-45 0.12016451847094986 46-47 0.138098464632394 48-49 0.1486370177320005 50-51 0.1196194208968323 52-53 0.09055698919626047 54-55 0.09306302219934648 56-57 0.09794058360930766 58-59 0.09431131924137331 60-61 0.09449065870298776 62-63 0.08384355803450935 64-65 0.10587635478574667 66-67 0.10354966124427512 68-69 0.10151557419280606 70-71 0.10180582095305048 72-73 0.09936822011294895 74-75 0.13734807056932302 76-77 0.1540832740136601 78-79 0.1526155221041314 80-81 0.12311890012807199 82-83 0.10302344150822222 84-85 0.1068013688509159 86-87 0.11296734270879137 88-89 0.1095740513166655 90-91 0.11393955136912229 92-93 0.10077933850828362 94-95 0.09679847440639464 96-97 0.10067551039892789 98-99 0.08936532566842767 100-101 0.09641383845582682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.1188867E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.96530658848236 #Duplication Level Percentage of deduplicated Percentage of total 1 74.38644448326085 37.91127949116511 2 13.127366733177869 13.380805405117085 3 4.705782530460371 7.194949482109115 4 2.292371635799193 4.673256929329868 5 1.3464147896319814 3.431022127443046 6 0.8416693431422577 2.573756167162304 7 0.6100213500479446 2.1762947591499393 8 0.47590716707788144 1.9403803742224262 9 0.30919508236778886 1.4182399951672875 >10 1.7559342927743138 15.813924685956234 >50 0.09145069252141512 3.171896156293502 >100 0.05441964453454038 4.883932098110903 >500 0.0023518536402509214 0.7892773525819928 >1k 6.504489904730941E-4 0.5648353953786229 >5k 1.9952572812434656E-5 0.07614958081247213 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.015569496943843198 3.775567612935604E-5 9.43891903233901E-6 2 0.015871542352878045 4.247513564552555E-5 1.4158378548508516E-5 3 0.017523353183537375 5.191405467786456E-5 1.4158378548508516E-5 4 0.02338020244310373 5.663351419403406E-5 2.3597297580847528E-5 5 0.02441376407714485 6.135297371020358E-5 2.831675709701703E-5 6 0.027311512220072928 8.023081177488159E-5 3.775567612935604E-5 7 0.027934480876207302 8.023081177488159E-5 3.775567612935604E-5 8 0.029435269002349204 8.49502712910511E-5 5.191405467786456E-5 9 0.03155902578462548 1.0382810935572913E-4 5.191405467786456E-5 10-11 0.03560832204949892 1.1090729862998337E-4 5.4273784435949316E-5 12-13 0.04010124750889229 1.2270594742040713E-4 6.843216298445782E-5 14-15 0.04490329756659476 1.3686432596891565E-4 8.49502712910511E-5 16-17 0.04986580924784699 1.5810189379167843E-4 8.731000104913585E-5 18-19 0.05464426200796862 1.651810830659327E-4 8.96697308072206E-5 20-21 0.05863456502888994 1.7697973185635646E-4 8.96697308072206E-5 22-23 0.06237473669545427 1.8641865088869547E-4 9.910864983955962E-5 24-25 0.06567599862701484 1.9113811040486498E-4 1.0618783911381388E-4 26-27 0.06901265650494667 1.9585756992103448E-4 1.0854756887189862E-4 28-29 0.07167915113158244 1.9821729967911924E-4 1.0854756887189862E-4 30-31 0.07398696683498934 2.1473540798571248E-4 1.3214486645274614E-4 32-33 0.07602577334597457 2.2181459725996675E-4 1.3686432596891565E-4 34-35 0.07778377201574771 2.2181459725996675E-4 1.6282135330784794E-4 36-37 0.07961020284850531 2.2181459725996675E-4 1.746200020982717E-4 38-39 0.08140359746464972 2.2181459725996675E-4 1.746200020982717E-4 40-41 0.08310496262022882 2.3125351629230576E-4 1.8641865088869547E-4 42-43 0.08456327561072519 2.47771624598899E-4 2.336132460503905E-4 44-45 0.08617969049501326 2.5485081387315327E-4 2.7608838169591605E-4 46-47 0.08791409186720554 2.57210543631238E-4 2.784481114540008E-4 48-49 0.08967916972625295 2.7608838169591605E-4 2.831675709701703E-4 50-51 0.0913569375842512 3.044051387929331E-4 2.878870304863398E-4 52-53 0.09287896327821588 3.1148432806718735E-4 2.949662197605941E-4 54-55 0.09458504789331114 3.138440578252721E-4 2.9732594951867883E-4 56-57 0.0964020398070364 3.4216081492228914E-4 3.0676486855101784E-4 58-59 0.09811520361140594 3.5159973395462816E-4 3.232829768576111E-4 60-61 0.10005018201303542 3.8227622080972995E-4 3.3744135540611964E-4 62-63 0.10204179392885895 4.2711108621334027E-4 3.4688027443845865E-4 64-65 0.10422454395508736 4.38909735003764E-4 3.492400041965434E-4 66-67 0.10665978506543082 4.436291945199335E-4 3.5631919347079767E-4 68-69 0.10930740185400192 4.48348654036103E-4 3.633983827450519E-4 70-71 0.11221458891596234 4.530681135522725E-4 3.633983827450519E-4 72-73 0.11580373787800924 4.530681135522725E-4 3.8227622080972995E-4 74-75 0.11998281927957734 4.6486676234269627E-4 3.893554100839842E-4 76-77 0.12491229474421639 4.743056813750353E-4 3.964345993582385E-4 78-79 0.13113726184604396 4.837446004073743E-4 4.0351378863249274E-4 80-81 0.13921461680796807 4.908237896816286E-4 4.318305457295098E-4 82-83 0.1501189280200777 4.955432491977981E-4 4.837446004073743E-4 84-85 0.16436461656963536 4.955432491977981E-4 4.931835194397134E-4 86-87 0.18231980029890224 4.955432491977981E-4 5.002627087139676E-4 88-89 0.20500152273361288 5.191405467786455E-4 5.049821682301371E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 1630 0.0 13.401041 1 CGAATTA 6215 0.0 12.461072 14-15 CTCGGTT 9030 0.0 12.042482 1 CTCGGAT 11330 0.0 11.274327 1 CTCGCAT 8465 0.0 11.163347 1 CTCGGGT 11110 0.0 11.155646 1 CTCGAAT 10055 0.0 10.767653 1 CTCGTTT 9000 0.0 10.710797 1 CTCGTAT 5405 0.0 10.630604 1 CGGAAAT 6550 0.0 10.512234 1 CTCGATT 11035 0.0 10.41385 1 CTCGACT 11080 0.0 10.328698 1 CCCGGTT 7260 0.0 10.203668 1 CGCGAAT 3125 0.0 10.181062 1 CTGGAAT 19115 0.0 9.564334 1 CCGGGAT 8830 0.0 9.51877 1 CTTGAAT 17185 0.0 9.477917 1 CTTGGAT 25090 0.0 9.444265 1 CGACAAT 7340 0.0 9.380808 1 AATTAGG 8700 0.0 9.283547 16-17 >>END_MODULE