FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 kx12-3.3420000000cd67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 kx12-3.3420000000cd67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21188867
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT16300.013.4010411
CGAATTA62150.012.46107214-15
CTCGGTT90300.012.0424821
CTCGGAT113300.011.2743271
CTCGCAT84650.011.1633471
CTCGGGT111100.011.1556461
CTCGAAT100550.010.7676531
CTCGTTT90000.010.7107971
CTCGTAT54050.010.6306041
CGGAAAT65500.010.5122341
CTCGATT110350.010.413851
CTCGACT110800.010.3286981
CCCGGTT72600.010.2036681
CGCGAAT31250.010.1810621
CTGGAAT191150.09.5643341
CCGGGAT88300.09.518771
CTTGAAT171850.09.4779171
CTTGGAT250900.09.4442651
CGACAAT73400.09.3808081
AATTAGG87000.09.28354716-17
Produced by FastQC (version 0.11.2)