##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 kx0-3.3420000000cb5d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19951310 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.308788645958586 33.0 33.0 33.0 33.0 33.0 2 32.21731490313167 33.0 33.0 33.0 33.0 33.0 3 32.183471862248645 33.0 33.0 33.0 33.0 33.0 4 35.995462754074794 37.0 37.0 37.0 37.0 37.0 5 35.99089042273415 37.0 37.0 37.0 37.0 37.0 6 36.0305377441381 37.0 37.0 37.0 37.0 37.0 7 36.059903384790275 37.0 37.0 37.0 37.0 37.0 8 36.03925261048021 37.0 37.0 37.0 37.0 37.0 9 35.991044247219854 37.0 37.0 37.0 37.0 37.0 10-11 35.9769630665856 37.0 37.0 37.0 37.0 37.0 12-13 35.97635759255908 37.0 37.0 37.0 37.0 37.0 14-15 38.163210310500915 40.0 37.0 40.0 37.0 40.0 16-17 38.1396685480803 40.0 37.0 40.0 37.0 40.0 18-19 38.12485834263514 40.0 37.0 40.0 37.0 40.0 20-21 38.090461252920235 40.0 37.0 40.0 37.0 40.0 22-23 38.152604941730644 40.0 37.0 40.0 37.0 40.0 24-25 38.085664074188614 40.0 37.0 40.0 37.0 40.0 26-27 38.01348941498077 40.0 37.0 40.0 37.0 40.0 28-29 37.95121463202166 40.0 37.0 40.0 37.0 40.0 30-31 37.91382187435311 40.0 37.0 40.0 33.0 40.0 32-33 37.82432734993341 40.0 37.0 40.0 33.0 40.0 34-35 37.75517487322888 40.0 37.0 40.0 33.0 40.0 36-37 37.70396021614621 40.0 37.0 40.0 33.0 40.0 38-39 37.64219825665583 40.0 37.0 40.0 33.0 40.0 40-41 37.54853087341132 40.0 37.0 40.0 33.0 40.0 42-43 37.457054624483305 40.0 37.0 40.0 33.0 40.0 44-45 37.39790657856552 40.0 37.0 40.0 33.0 40.0 46-47 37.23653674871474 40.0 37.0 40.0 33.0 40.0 48-49 37.11555211161573 40.0 37.0 40.0 33.0 40.0 50-51 35.722213253164824 38.5 35.0 38.5 30.0 40.0 52-53 36.043732992971385 37.0 37.0 40.0 33.0 40.0 54-55 36.75060161463082 38.5 37.0 40.0 33.0 40.0 56-57 36.75965668419768 37.0 37.0 40.0 33.0 40.0 58-59 36.63561908967381 37.0 37.0 40.0 33.0 40.0 60-61 36.538881832822 37.0 37.0 40.0 33.0 40.0 62-63 36.414473034602736 37.0 37.0 40.0 33.0 40.0 64-65 36.203393937540945 37.0 37.0 40.0 33.0 40.0 66-67 35.938487698301515 37.0 37.0 40.0 33.0 40.0 68-69 35.67386404702248 37.0 37.0 38.5 33.0 40.0 70-71 35.42855915225617 37.0 37.0 37.0 33.0 40.0 72-73 35.20547462798182 37.0 37.0 37.0 33.0 40.0 74-75 34.96376696567795 37.0 35.0 37.0 27.0 40.0 76-77 34.75881668922993 37.0 33.0 37.0 27.0 37.0 78-79 34.57329413457062 37.0 33.0 37.0 27.0 37.0 80-81 34.409390761809625 37.0 33.0 37.0 27.0 37.0 82-83 34.2897971361279 37.0 33.0 37.0 27.0 37.0 84-85 34.18723194116076 37.0 33.0 37.0 27.0 37.0 86-87 34.08321588908197 37.0 33.0 37.0 27.0 37.0 88-89 33.99912820260925 37.0 33.0 37.0 27.0 37.0 90-91 33.916270560679976 37.0 33.0 37.0 27.0 37.0 92-93 33.84469714018779 37.0 33.0 37.0 27.0 37.0 94-95 33.77133448881301 37.0 33.0 37.0 27.0 37.0 96-97 33.685100627477595 37.0 33.0 37.0 27.0 37.0 98-99 33.62930456696829 37.0 33.0 37.0 27.0 37.0 100-101 31.91322259039632 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11977828918101352 2306 2 0.22716029358952738 2306 3 0.2644271341488249 2306 4 0.33815541326281817 2306 5 0.3552018238612007 2306 6 0.3733695065015823 2306 7 0.3991174781190807 2306 8 0.3677014196319206 2306 9 0.38706485447649186 2306 10-11 0.3856758711170656 2306 12-13 0.3507712593789236 2306 14-15 0.6851230401529023 2306 16-17 0.6933510321319574 2306 18-19 0.7108471949778377 2306 20-21 0.7191032475104819 2306 22-23 0.7135474532285286 2306 24-25 0.72819176742113 2306 26-27 0.7312961164345211 2306 28-29 0.7293628984644229 2306 30-31 0.7215572224024456 2306 32-33 0.8060663162661115 2306 34-35 0.7796565005943208 2306 36-37 0.7848263481444135 2306 38-39 0.7410559742590124 2306 40-41 0.7745855792537029 2306 42-43 0.7489961986380251 2306 44-45 0.8172865373071048 2306 46-47 0.8010934170968156 2306 48-49 0.776077874667358 2306 50-51 0.6700074108743266 2306 52-53 0.7632232032018109 2306 54-55 0.8399593748910092 2306 56-57 0.8310719845326915 2306 58-59 0.8505816323624273 2306 60-61 0.8043995967428685 2306 62-63 0.8104146154164482 2306 64-65 0.8321641332401626 2306 66-67 0.7664959464862164 2306 68-69 0.8221277621485186 2306 70-71 0.818061452154538 2306 72-73 0.8176099907492542 2306 74-75 0.8123137327953103 2306 76-77 0.8210596516488167 2306 78-79 0.7823449657555557 2306 80-81 0.8403185811872973 2306 82-83 0.854045762327452 2306 84-85 0.8979428701772321 2306 86-87 0.8543118072900171 2306 88-89 0.8612661808955053 2306 90-91 0.8790056329627873 2306 92-93 0.9008679921788953 2306 94-95 0.8839616107931363 2306 96-97 0.8612156985899873 2306 98-99 0.8930001237617589 2306 100-101 0.7147575756211779 2307 1 0.19397405664700784 2307 2 0.25910226580854356 2307 3 0.24594083600505456 2307 4 0.35181502360435957 2307 5 0.3805963553090663 2307 6 0.3302946907073405 2307 7 0.37457168977184097 2307 8 0.32776490617072085 2307 9 0.3214871860336075 2307 10-11 0.31577667906450557 2307 12-13 0.35003882909883544 2307 14-15 0.6228751782469502 2307 16-17 0.5861373463123485 2307 18-19 0.6098299517177921 2307 20-21 0.6100880055707734 2307 22-23 0.6641327585097443 2307 24-25 0.6713630761967764 2307 26-27 0.6283916797961098 2307 28-29 0.6294176394729263 2307 30-31 0.6391884615795576 2307 32-33 0.6314253065178193 2307 34-35 0.6425819466825757 2307 36-37 0.6549833452307112 2307 38-39 0.6129121389751191 2307 40-41 0.6707385652921758 2307 42-43 0.654210571321201 2307 44-45 0.717888625347122 2307 46-47 0.7012237593368909 2307 48-49 0.6339948705262088 2307 50-51 0.5805322814095817 2307 52-53 0.6145267412007343 2307 54-55 0.7343196638360965 2307 56-57 0.7126207862100813 2307 58-59 0.7260927078272559 2307 60-61 0.6890206521377422 2307 62-63 0.7192937141875504 2307 64-65 0.7341930185013723 2307 66-67 0.7570050914608757 2307 68-69 0.7596007620839984 2307 70-71 0.7060330166754696 2307 72-73 0.672717519156457 2307 74-75 0.7151986648624344 2307 76-77 0.695441437658026 2307 78-79 0.7460494702396048 2307 80-81 0.7458232314932403 2307 82-83 0.6708747078678741 2307 84-85 0.7870052299487185 2307 86-87 0.7450059199150516 2307 88-89 0.7361221823028572 2307 90-91 0.7461566666525243 2307 92-93 0.7277695565169751 2307 94-95 0.7394860178846372 2307 96-97 0.7930113549976241 2307 98-99 0.7199076992069351 2307 100-101 0.7269513690900453 2201 1 0.23015481331959364 2201 2 0.302122951702934 2201 3 0.31247788766878415 2201 4 0.4044578982671041 2201 5 0.4079108794652342 2201 6 0.4013419206742057 2201 7 0.3928094507926758 2201 8 0.3314510953471128 2201 9 0.38511600106894406 2201 10-11 0.39693120707612906 2201 12-13 0.43148152171550436 2201 14-15 0.6934150026066206 2201 16-17 0.7173686457102519 2201 18-19 0.6645622213530018 2201 20-21 0.7224263806126885 2201 22-23 0.7286655125510251 2201 24-25 0.7616317216674915 2201 26-27 0.7098955794974984 2201 28-29 0.6962213845064227 2201 30-31 0.7357808452729699 2201 32-33 0.7608147302186126 2201 34-35 0.7895384412718229 2201 36-37 0.7843864017594981 2201 38-39 0.7503047734225561 2201 40-41 0.7505114615049067 2201 42-43 0.7307476117336336 2201 44-45 0.7608604899654523 2201 46-47 0.7987510981263526 2201 48-49 0.8107832275285318 2201 50-51 0.7319599591632766 2201 52-53 0.7899486931666715 2201 54-55 0.8692970280910117 2201 56-57 0.8501673478495917 2201 58-59 0.7964867996933904 2201 60-61 0.8480049952004549 2201 62-63 0.8897507945946117 2201 64-65 0.8898227576436781 2201 66-67 0.8853348300031172 2201 68-69 0.8353167199561184 2201 70-71 0.7985869704206152 2201 72-73 0.7857523498131158 2201 74-75 0.8424617049343297 2201 76-77 0.8664734783667356 2201 78-79 0.8537307624741857 2201 80-81 0.842565088738219 2201 82-83 0.8729053804182172 2201 84-85 0.8570870137901991 2201 86-87 0.8666083922762269 2201 88-89 0.8476860176918066 2201 90-91 0.8365574399170299 2201 92-93 0.8930440807931532 2201 94-95 0.8415361176836953 2201 96-97 0.844171289260025 2201 98-99 0.860453426749892 2201 100-101 0.8114306100621889 1101 1 0.2087851423487237 1101 2 0.240926366843766 1101 3 0.1805183567888733 1101 4 0.2872070722431914 1101 5 0.2889414428406951 1101 6 0.3270692644155204 1101 7 0.3451752125021983 1101 8 0.3125309660968085 1101 9 0.3059028865722482 1101 10-11 0.3327217666439495 1101 12-13 0.32169889014539876 1101 14-15 0.5459280458355096 1101 16-17 0.53611706328374 1101 18-19 0.5387251926925671 1101 20-21 0.5605206587765039 1101 22-23 0.5918661090590334 1101 24-25 0.592775230224099 1101 26-27 0.5551634427348517 1101 28-29 0.5816638129676548 1101 30-31 0.6312540504987254 1101 32-33 0.6289377881001457 1101 34-35 0.6382331833741901 1101 36-37 0.6152799223648984 1101 38-39 0.5767407389322514 1101 40-41 0.6228383028823643 1101 42-43 0.6359719199420084 1101 44-45 0.6495449900473389 1101 46-47 0.6460377859421911 1101 48-49 0.63670486762134 1101 50-51 0.6009949499527707 1101 52-53 0.6584797962845101 1101 54-55 0.7077790102910626 1101 56-57 0.670834931205988 1101 58-59 0.6712048360953631 1101 60-61 0.7230266857564445 1101 62-63 0.7481727274567689 1101 64-65 0.7357282283633637 1101 66-67 0.6882009083614946 1101 68-69 0.6733026162152385 1101 70-71 0.6957423291028633 1101 72-73 0.6638105710054276 1101 74-75 0.6579535896078497 1101 76-77 0.7141611947679891 1101 78-79 0.7251292769505113 1101 80-81 0.7121648490489818 1101 82-83 0.7128048269587524 1101 84-85 0.6645355703446185 1101 86-87 0.6971477382960103 1101 88-89 0.7176833009885186 1101 90-91 0.6567219670231026 1101 92-93 0.6895749493683994 1101 94-95 0.6681020738749339 1101 96-97 0.694356219173109 1101 98-99 0.7151084076124832 1101 100-101 0.7207113548237984 2304 1 0.17202420516388628 2304 2 0.22594291221378882 2304 3 0.2776107929964482 2304 4 0.344730497824024 2304 5 0.35624307511845643 2304 6 0.392433327694512 2304 7 0.33300321828853896 2304 8 0.3853434485008691 2304 9 0.32465477216621963 2304 10-11 0.39322050475322357 2304 12-13 0.3968460093901456 2304 14-15 0.6531112035977671 2304 16-17 0.639855543799392 2304 18-19 0.6010108834758867 2304 20-21 0.6381845080854092 2304 22-23 0.6662769630659042 2304 24-25 0.6285091627844892 2304 26-27 0.6691028630792673 2304 28-29 0.6967145279388802 2304 30-31 0.7052273490258258 2304 32-33 0.7057429758167331 2304 34-35 0.708679841029813 2304 36-37 0.7599823267826622 2304 38-39 0.7219100747640255 2304 40-41 0.737130162189942 2304 42-43 0.7353120760597989 2304 44-45 0.741109254008613 2304 46-47 0.7291183581217027 2304 48-49 0.7477934611966219 2304 50-51 0.6864886200270348 2304 52-53 0.7929805672951318 2304 54-55 0.8245265705453164 2304 56-57 0.7771115849173356 2304 58-59 0.7815000453633871 2304 60-61 0.7806851972861466 2304 62-63 0.817819122819536 2304 64-65 0.8116446271768041 2304 66-67 0.7972506473754635 2304 68-69 0.7719613344659777 2304 70-71 0.767007157823052 2304 72-73 0.7731600341358771 2304 74-75 0.8142280227124203 2304 76-77 0.7872733319306988 2304 78-79 0.7899535368471859 2304 80-81 0.8239005107927468 2304 82-83 0.8081697212602421 2304 84-85 0.7819152030248162 2304 86-87 0.8447839387964322 2304 88-89 0.8376715513083965 2304 90-91 0.8398057706998685 2304 92-93 0.8349132495629235 2304 94-95 0.8529412492902324 2304 96-97 0.8536426868913694 2304 98-99 0.8226455591094322 2304 100-101 0.9657298263765739 2202 1 0.20380119992831425 2202 2 0.2222761251564478 2202 3 0.2531694483748339 2202 4 0.2817970795536482 2202 5 0.3014362831886146 2202 6 0.2665586704998404 2202 7 0.30144778975936504 2202 8 0.2847924763224867 2202 9 0.2284187533625257 2202 10-11 0.2569994779061062 2202 12-13 0.28716597367107966 2202 14-15 0.46437656170313346 2202 16-17 0.44950596530893705 2202 18-19 0.4261013328933245 2202 20-21 0.46122151447180215 2202 22-23 0.4484164380298594 2202 24-25 0.444800480721085 2202 26-27 0.4238028438357162 2202 28-29 0.4841378499820337 2202 30-31 0.49625069344079265 2202 32-33 0.5162700926307622 2202 34-35 0.502828451465497 2202 36-37 0.4533599250171889 2202 38-39 0.50946003926299 2202 40-41 0.5044773395026496 2202 42-43 0.48899537180659536 2202 44-45 0.4947283208576678 2202 46-47 0.47442066194415844 2202 48-49 0.4616386918774822 2202 50-51 0.5163778463889912 2202 52-53 0.5657310581004111 2202 54-55 0.6164080587256962 2202 56-57 0.6138626909473999 2202 58-59 0.5798955126071732 2202 60-61 0.5829903753174079 2202 62-63 0.5848476345538387 2202 64-65 0.6198176608037187 2202 66-67 0.5637656739325081 2202 68-69 0.5962786814347467 2202 70-71 0.5368651774059643 2202 72-73 0.6382335719816794 2202 74-75 0.6246494564231426 2202 76-77 0.6096061571585167 2202 78-79 0.5866698954748841 2202 80-81 0.6041602655180824 2202 82-83 0.6040765805899042 2202 84-85 0.6224160282223039 2202 86-87 0.5930214240912406 2202 88-89 0.6314736404183492 2202 90-91 0.6271657075279506 2202 92-93 0.6214758636141298 2202 94-95 0.6071512257903535 2202 96-97 0.6278571093147391 2202 98-99 0.5976795386657656 2202 100-101 0.6260360073474516 1102 1 0.08621808833074596 1102 2 0.09669251452235983 1102 3 0.11566318420837973 1102 4 0.142192450675374 1102 5 0.14923020626569894 1102 6 0.19268145025908723 1102 7 0.18775760337550906 1102 8 0.186802503182399 1102 9 0.17496351253546294 1102 10-11 0.16646298219090028 1102 12-13 0.16301262687634477 1102 14-15 0.3017420996666047 1102 16-17 0.2867822742838442 1102 18-19 0.23911404574393202 1102 20-21 0.2746471381101898 1102 22-23 0.29523287855749913 1102 24-25 0.2996930410929721 1102 26-27 0.2861344871545981 1102 28-29 0.31659823633695083 1102 30-31 0.2653471542653776 1102 32-33 0.32863006361115765 1102 34-35 0.29132592168581084 1102 36-37 0.3770668123502574 1102 38-39 0.3362597264216092 1102 40-41 0.3197995011275836 1102 42-43 0.33381303476403446 1102 44-45 0.3305500222367286 1102 46-47 0.28485114384988464 1102 48-49 0.29622387300887 1102 50-51 0.31783204511036445 1102 52-53 0.3176222178151633 1102 54-55 0.4072867194626326 1102 56-57 0.4280558663084122 1102 58-59 0.4074648063446844 1102 60-61 0.3996199706994332 1102 62-63 0.3952679365338341 1102 64-65 0.3789676946669118 1102 66-67 0.3779453669202013 1102 68-69 0.33067038107346747 1102 70-71 0.3744836094667576 1102 72-73 0.358717008372345 1102 74-75 0.3393493111154484 1102 76-77 0.3524197691135882 1102 78-79 0.3181541936471035 1102 80-81 0.348904582687247 1102 82-83 0.34863205017698107 1102 84-85 0.355287060829518 1102 86-87 0.28977239228499485 1102 88-89 0.33949600899666166 1102 90-91 0.3346081453423366 1102 92-93 0.30218618339589653 1102 94-95 0.3828755102076187 1102 96-97 0.3937715098733321 1102 98-99 0.3970759351188349 1102 100-101 0.48394221364244316 2305 1 0.14396405932196643 2305 2 0.10967759535279953 2305 3 0.14950446443962306 2305 4 0.1322715113929931 2305 5 0.1863100168093439 2305 6 0.2001778609560958 2305 7 0.23071155184437941 2305 8 0.1856720102994487 2305 9 0.14253868027687133 2305 10-11 0.18273517509344828 2305 12-13 0.19175372065328133 2305 14-15 0.32324760819891196 2305 16-17 0.2774466801897333 2305 18-19 0.2570052093276658 2305 20-21 0.31181486268000214 2305 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-1.2324822048379716 1213 96-97 -1.1419344909682607 1213 98-99 -1.0066365088940543 1213 100-101 -0.9044677113951529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15849.0 3 1353.0 4 1154.0 5 967.0 6 772.0 7 1242.0 8 3404.0 9 18792.0 10 73263.0 11 80201.0 12 37799.0 13 26156.0 14 25160.0 15 26811.0 16 29850.0 17 33934.0 18 38030.0 19 43342.0 20 48699.0 21 54914.0 22 61483.0 23 70617.0 24 81645.0 25 95325.0 26 115385.0 27 145277.0 28 159890.0 29 184110.0 30 228964.0 31 291423.0 32 383867.0 33 519691.0 34 724469.0 35 1120047.0 36 2259345.0 37 6062157.0 38 6877795.0 39 8128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.663570472319982 13.007267310308512 12.713337891498675 52.61582432587283 2 22.942356737376215 18.331622118134096 39.14253395061156 19.58348719387813 3 23.9908305506571 23.973407312612775 26.03095978462811 26.00480235210202 4 26.13630205584067 28.084895000190674 19.76268417365963 26.016118770309028 5 28.16948736230559 29.57057745914445 22.68509441773373 19.574840760816233 6 21.40601041148044 32.58071746944153 23.757011875814367 22.256260243263654 7 19.530122314162703 14.874926050714546 43.197455968973586 22.39749566614916 8 21.083882040679626 21.197700395655236 28.68921870080522 29.029198862859918 9 21.473420679044406 20.738830502852558 32.0867876941204 25.700961123982637 10-11 25.059630215532145 29.059333930034732 22.160045689972847 23.72099016446027 12-13 23.05366348224477 23.093669097578413 28.95392990961013 24.898737510566686 14-15 23.731367130987053 24.734944699053333 27.31308334230676 24.220604827652856 16-17 24.94148769011215 24.965076557040252 25.85542077306388 24.238014979783713 18-19 24.619342315018415 25.272018791429634 25.83920690972972 24.269431983822226 20-21 24.30868078761482 25.67622273382859 26.153440101110643 23.861656377445943 22-23 24.66953337877437 25.449482712709127 25.846940821807923 24.034043086708575 24-25 24.13943117786454 25.89963851657445 25.895280574096326 24.06564973146469 26-27 23.711673331137977 26.507208605792602 25.94996471750482 23.831153345564598 28-29 24.2919922518101 25.808956839487518 25.70083911964375 24.198211789058632 30-31 24.410819988593275 25.48403641733723 25.942593075089103 24.162550518980392 32-33 24.384740498049432 25.723624657713863 26.03424225015956 23.857392594077144 34-35 24.51459352441385 25.864136124353752 25.597793494461378 24.023476856771016 36-37 23.708997138820177 25.953720962693062 26.05719795368423 24.280083944802534 38-39 23.848234633683646 25.6134165285743 26.467476095402652 24.070872742339404 40-41 24.582427339945184 25.36963701985739 25.721779124797962 24.326156515399468 42-43 24.50375951093939 25.411908185911763 25.987801286807215 24.096531016341633 44-45 24.922072229811672 25.51253373223359 25.862105911108795 23.703288126845944 46-47 24.584695471453692 25.363444086477855 25.71321252080146 24.338647921266993 48-49 24.0205955122238 25.337919986089148 25.6529296321254 24.988554869561654 50-51 24.33419538231205 25.491412581017197 25.8353213453424 24.339070691328356 52-53 24.163064654804366 25.650825406632933 26.058717567720652 24.127392370842053 54-55 23.596274426664827 25.60559179662861 26.17739867641742 24.620735100289146 56-57 23.725829877649957 25.694866362556994 26.707567668766558 23.87173609102649 58-59 23.900203235298854 25.93836093985025 26.15163031996327 24.009805504887627 60-61 23.600117288255205 26.540569166117784 25.74269998135419 24.116613564272825 62-63 23.8363764174936 26.770434474551973 25.72793553917869 23.66525356877574 64-65 23.743940786480607 26.75729171626792 25.608959520010586 23.889807977240885 66-67 23.62846319941227 26.654880699128025 25.6174298920663 24.099226209393404 68-69 23.88578421966155 26.632020185399437 25.70255195222372 23.7796436427153 70-71 23.991646001181604 26.48158169044295 25.517231690505664 24.00954061786978 72-73 23.819843088354887 26.414993046895656 25.625859040720204 24.13930482402925 74-75 23.948373280662384 26.465724322849216 25.701004590421444 23.884897806066956 76-77 24.017056205634304 26.328489337800697 25.572338083453474 24.082116373111525 78-79 23.87600070421344 26.23498771115422 25.685841288825053 24.203170295807293 80-81 24.047320918363905 26.3381343811443 25.593718995813585 24.020825704678213 82-83 24.121448776115496 26.293396711501554 25.445906215617697 24.13924829676525 84-85 23.8963827819552 26.27982596842558 25.646028921868385 24.177762327750834 86-87 24.100399203134252 26.412791180071803 25.705458795321263 23.78135082147268 88-89 24.168214473066786 26.172839840693545 25.402357533004462 24.256588153235203 90-91 24.090659854731186 26.104573934802506 25.503539990824038 24.301226219642267 92-93 24.142013087174423 26.293882371369637 25.590044981109898 23.97405956034604 94-95 24.293698544803792 26.226710340832188 25.417241990194533 24.062349124169486 96-97 24.018439991768524 26.27067392614696 25.523898010876277 24.18698807120824 98-99 24.278912252299754 26.352120579247146 25.47569710002676 23.893270068426332 100-101 24.556648122663304 26.225049011994795 25.161135454225064 24.057167411116833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13751.0 1 7492.0 2 918.5 3 526.5 4 459.5 5 441.5 6 439.5 7 451.0 8 445.5 9 501.0 10 552.5 11 561.5 12 582.5 13 659.0 14 760.5 15 848.0 16 1019.0 17 1280.0 18 1633.5 19 2033.0 20 2654.0 21 3862.0 22 5725.0 23 8435.5 24 12447.5 25 18340.0 26 26335.5 27 36868.5 28 50282.5 29 67430.5 30 88227.5 31 113376.5 32 143318.5 33 179766.5 34 225325.0 35 281605.5 36 350794.5 37 432819.0 38 526156.5 39 624422.0 40 718534.0 41 805191.5 42 881731.0 43 924838.0 44 935980.0 45 919272.5 46 877708.0 47 823665.0 48 765042.0 49 710791.5 50 654797.5 51 604201.5 52 563065.0 53 532207.5 54 513378.0 55 495796.5 56 480952.0 57 459797.5 58 424538.5 59 399914.0 60 378906.0 61 348630.0 62 327784.0 63 306044.0 64 267997.5 65 237812.0 66 221037.5 67 205047.0 68 188869.5 69 162634.0 70 133469.0 71 110195.0 72 89140.0 73 72754.0 74 55601.5 75 39798.5 76 28734.5 77 20164.0 78 13529.5 79 8766.0 80 5627.0 81 3466.0 82 2177.5 83 1372.0 84 863.0 85 602.0 86 470.5 87 373.0 88 315.0 89 257.5 90 203.5 91 164.0 92 116.5 93 90.5 94 77.0 95 55.5 96 39.0 97 25.5 98 12.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09680567341192133 2 0.0858740604000439 3 0.09102660426809066 4 0.10901539798639788 5 0.09223454499980202 6 0.09371815685285828 7 0.10235418125426349 8 0.08918712605838915 9 0.11828797206799954 10-11 0.08938510804553686 12-13 0.08324516034285469 14-15 0.1340338052990004 16-17 0.13360776811146738 18-19 0.12633255660906478 20-21 0.1392865932111726 22-23 0.13695090698305024 24-25 0.11117315103619764 26-27 0.11464159496293727 28-29 0.10385032361283546 30-31 0.09188118474425991 32-33 0.11340358101798828 34-35 0.13237727246982778 36-37 0.11874658856987336 38-39 0.12093692093401386 40-41 0.1493987111623247 42-43 0.12421239507581205 44-45 0.12291924690659412 46-47 0.14098572975909854 48-49 0.1512381893720262 50-51 0.1221373433624158 52-53 0.09256535034541591 54-55 0.09507395754965464 56-57 0.10031170885520799 58-59 0.09643978265086353 60-61 0.09633703250563497 62-63 0.08567357231179307 64-65 0.10794779891646213 66-67 0.10581761297879688 68-69 0.10358467689590307 70-71 0.10392801274703264 72-73 0.10167001565310749 74-75 0.13967503888215863 76-77 0.15681927652870914 78-79 0.15644586746434194 80-81 0.12565340321011503 82-83 0.10524622192728196 84-85 0.10938128874745567 86-87 0.11520296161003964 88-89 0.1117696030987439 90-91 0.11603749327738377 92-93 0.10274513302635266 94-95 0.09909624982018725 96-97 0.10274262692524952 98-99 0.0911970191431039 100-101 0.09878799938450157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.995131E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.6168565756689 #Duplication Level Percentage of deduplicated Percentage of total 1 74.45580422329023 37.68718763596364 2 13.20310714415784 13.365995613380537 3 4.805812522569002 7.297653695532862 4 2.056554157835335 4.163852273889868 5 1.4594700884739804 3.6936894072383133 6 0.8817378984953765 2.67784804472833 7 0.5424487683875442 1.9219936056384395 8 0.4104194527779135 1.6619314061699328 9 0.31859908796823533 1.4513835906744381 >10 1.6961903801082776 15.311060858925416 >50 0.10903641183335759 3.8322042838885135 >100 0.05801913495569015 5.321875719127454 >500 0.0021237040511222706 0.7403530207502199 >1k 6.446714117709788E-4 0.5749138630048536 >5k 2.2427361505553432E-5 0.060569018450179465 >10k+ 9.92632274978227E-6 0.2374879626369936 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 47267 0.23691176168381925 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.2515774653393687 3.0073213237627003E-5 5.012202206271167E-6 2 0.25286560130638036 3.0073213237627003E-5 3.0073213237627003E-5 3 0.25344701676230785 3.508541544389817E-5 3.0073213237627003E-5 4 0.25544187324040374 3.508541544389817E-5 3.508541544389817E-5 5 0.2558578860235243 3.508541544389817E-5 4.009761765016934E-5 6 0.2570307413397917 7.017083088779634E-5 5.513422426898284E-5 7 0.25732144906775545 7.518303309406751E-5 5.513422426898284E-5 8 0.2583589749244536 8.019523530033868E-5 6.014642647525401E-5 9 0.2593112933436451 8.019523530033868E-5 6.515862868152518E-5 10-11 0.2613011376195348 9.523184191915218E-5 7.017083088779634E-5 12-13 0.2629175728310572 1.1778675184737243E-4 8.771353860974543E-5 14-15 0.2646818680076647 1.403416617755927E-4 9.021963971288101E-5 16-17 0.26662409636259476 1.6039047060067735E-4 9.523184191915218E-5 18-19 0.2683307512138301 1.6039047060067735E-4 9.523184191915218E-5 20-21 0.2698845338977741 1.6289657170381292E-4 9.523184191915218E-5 22-23 0.27141074946958366 1.679087739100841E-4 1.0525624633169451E-4 24-25 0.27242321431525046 1.80439279425762E-4 1.1026844853796568E-4 26-27 0.2733705205322357 1.9547588604457551E-4 1.227989540536436E-4 28-29 0.27430028404149903 1.9798198714771108E-4 1.3532945956932151E-4 30-31 0.27535034040371287 2.0550029045711786E-4 1.528721672912706E-4 32-33 0.27615229275671627 2.280552003853381E-4 2.0800639156025346E-4 34-35 0.2768113973468409 2.4309180700415162E-4 2.5311621141669395E-4 36-37 0.2776008191943286 2.606345147261007E-4 2.7316502024177865E-4 38-39 0.27845790577160096 2.606345147261007E-4 2.907077279637277E-4 40-41 0.27933002895549214 2.6314061582923627E-4 3.2328704230449027E-4 42-43 0.2801645606228363 2.7567112134491416E-4 3.5085415443898173E-4 44-45 0.28098656178466475 2.932138290668633E-4 3.633846599546596E-4 46-47 0.281748416520018 3.2328704230449027E-4 3.6839686216093076E-4 48-49 0.2827057471414158 3.383236489233038E-4 3.7842126657347314E-4 50-51 0.2847331829338524 3.43335851129575E-4 3.8844567098601546E-4 52-53 0.2869385519046118 3.4584195223271054E-4 3.9596397429542216E-4 54-55 0.2893444089636219 3.5336025554211724E-4 4.0598837870796454E-4 56-57 0.2905874351107772 3.6589076105779524E-4 4.1100058091423573E-4 58-59 0.29216126660354635 3.709029632640664E-4 4.2102498532677805E-4 60-61 0.2942638854290771 3.759151654703375E-4 4.3606159194559157E-4 62-63 0.29754186567197843 4.110005809142357E-4 4.410737941518627E-4 64-65 0.30026850367218993 4.310493897393204E-4 4.460859963581339E-4 66-67 0.30367429507135124 4.410737941518627E-4 4.63628704080083E-4 68-69 0.3072530074466288 4.4859209746126946E-4 4.7615920959576087E-4 70-71 0.31215243510325885 4.7615920959576087E-4 4.8117141180203205E-4 72-73 0.31873846880229917 4.7615920959576087E-4 4.861836140083032E-4 74-75 0.3281213113324388 4.861836140083032E-4 4.886897151114388E-4 76-77 0.3387421678075274 4.962080184208455E-4 5.062324228333879E-4 78-79 0.3514606309059405 4.962080184208455E-4 5.262812316584725E-4 80-81 0.3686625088778631 5.137507261427946E-4 5.463300404835573E-4 82-83 0.3933651474514706 5.237751305553369E-4 5.914398603399977E-4 84-85 0.42730276859013266 5.363056360710149E-4 6.265252757838959E-4 86-87 0.4699541032643972 5.463300404835572E-4 6.315374779901671E-4 88-89 0.5210184193418879 5.688849504117775E-4 6.415618824027094E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGT 16040 0.0 29.971964 9 CGCCGTA 10150 0.0 27.874117 46-47 CCGTATC 10950 0.0 26.29148 48-49 GCCGTAT 9610 0.0 25.310637 48-49 CGTATCA 10180 0.0 23.956015 50-51 AAGAGCG 20275 0.0 23.916054 8 TCGGGGG 14340 0.0 23.871609 38-39 TTAAAAA 36180 0.0 23.775642 56-57 GAGTGTA 10425 0.0 23.07627 28-29 TAAAAAA 41285 0.0 22.695616 58-59 ATCGGAA 26450 0.0 22.587357 3 TCGTGTA 12210 0.0 22.511543 14-15 GTAGGGA 12720 0.0 22.261274 18-19 TTTAAAA 24060 0.0 21.69025 56-57 CGTGTAG 11705 0.0 20.903894 16-17 AGTGTAG 12130 0.0 20.85072 28-29 TCGGAAG 25905 0.0 20.276188 4 GATCGGA 30005 0.0 20.257528 2 TGTAGGG 12115 0.0 20.196432 18-19 GTCGTGT 12325 0.0 19.641508 14-15 >>END_MODULE