FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 kx0-3.3420000000cb5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 kx0-3.3420000000cb5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19951310
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC472670.23691176168381925Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT160400.029.9719649
CGCCGTA101500.027.87411746-47
CCGTATC109500.026.2914848-49
GCCGTAT96100.025.31063748-49
CGTATCA101800.023.95601550-51
AAGAGCG202750.023.9160548
TCGGGGG143400.023.87160938-39
TTAAAAA361800.023.77564256-57
GAGTGTA104250.023.0762728-29
TAAAAAA412850.022.69561658-59
ATCGGAA264500.022.5873573
TCGTGTA122100.022.51154314-15
GTAGGGA127200.022.26127418-19
TTTAAAA240600.021.6902556-57
CGTGTAG117050.020.90389416-17
AGTGTAG121300.020.8507228-29
TCGGAAG259050.020.2761884
GATCGGA300050.020.2575282
TGTAGGG121150.020.19643218-19
GTCGTGT123250.019.64150814-15
Produced by FastQC (version 0.11.2)