FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 k48-2.3420000000ce4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 k48-2.3420000000ce4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12586605
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC302380.24023952447860247Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA106050.030.9433983
AGAGCGT104200.030.04269
GCCGTAT53000.029.984448-49
AAGAGCG106150.029.8862158
AGATCGG109150.028.6705741
CGTGTAG58200.027.30554416-17
TCGTGTA59700.027.21767814-15
CCGTATC60450.027.19286248-49
GAGTGTA57000.027.164928-29
GTCGTGT59550.026.48839414-15
GATCGGA125200.026.474382
CGTATCA60500.026.2578250-51
CGCCGTA61450.026.2106146-47
GTAGGGA61950.026.18923218-19
TCGGAAG123650.025.8863964
CGCGTAT33250.025.5644281
CGCGTGT23900.024.8377763
TGTAGGG64150.024.73564118-19
ACATGCG37050.024.225874
CGGAAGA141350.022.8460045
Produced by FastQC (version 0.11.2)