##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l08n02 k12-3.3420000000cc5c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20792259 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.381785981023036 33.0 33.0 33.0 33.0 33.0 2 32.32933270983206 33.0 33.0 33.0 33.0 33.0 3 32.315070623158356 33.0 33.0 33.0 33.0 33.0 4 36.14417389664105 37.0 37.0 37.0 37.0 37.0 5 36.132883059988814 37.0 37.0 37.0 37.0 37.0 6 36.12674139928711 37.0 37.0 37.0 37.0 37.0 7 36.178275386046316 37.0 37.0 37.0 37.0 37.0 8 36.1648185028861 37.0 37.0 37.0 37.0 37.0 9 36.15088177768467 37.0 37.0 37.0 37.0 37.0 10-11 36.1273655979372 37.0 37.0 37.0 37.0 37.0 12-13 36.1261173449215 37.0 37.0 37.0 37.0 37.0 14-15 38.292030245487034 40.0 37.0 40.0 37.0 40.0 16-17 38.27142904481904 40.0 37.0 40.0 37.0 40.0 18-19 38.246232840789446 40.0 37.0 40.0 37.0 40.0 20-21 38.20797915705071 40.0 37.0 40.0 37.0 40.0 22-23 38.195335004243645 40.0 37.0 40.0 37.0 40.0 24-25 38.14941409204262 40.0 37.0 40.0 37.0 40.0 26-27 38.090462969896635 40.0 37.0 40.0 37.0 40.0 28-29 38.03280225587802 40.0 37.0 40.0 33.0 40.0 30-31 37.978495891187194 40.0 37.0 40.0 33.0 40.0 32-33 37.887009703947996 40.0 37.0 40.0 33.0 40.0 34-35 37.80802571764809 40.0 37.0 40.0 33.0 40.0 36-37 37.73605951137873 40.0 37.0 40.0 33.0 40.0 38-39 37.63802278530678 40.0 37.0 40.0 33.0 40.0 40-41 37.51462592400374 40.0 37.0 40.0 33.0 40.0 42-43 37.402692391432794 40.0 37.0 40.0 33.0 40.0 44-45 37.28695020584343 40.0 37.0 40.0 33.0 40.0 46-47 37.143360444865564 40.0 37.0 40.0 33.0 40.0 48-49 36.99331770540181 40.0 37.0 40.0 33.0 40.0 50-51 35.53869007210808 38.5 35.0 38.5 30.0 38.5 52-53 35.85973791977101 37.0 35.0 38.5 33.0 40.0 54-55 36.567642313420585 37.0 37.0 40.0 33.0 40.0 56-57 36.539464446840526 37.0 37.0 40.0 33.0 40.0 58-59 36.406097312466144 37.0 37.0 40.0 33.0 40.0 60-61 36.24228817561382 37.0 37.0 40.0 33.0 40.0 62-63 36.07218138250394 37.0 37.0 40.0 33.0 40.0 64-65 35.87227027616383 37.0 37.0 40.0 33.0 40.0 66-67 35.64623406239794 37.0 37.0 38.5 33.0 40.0 68-69 35.42686874475736 37.0 37.0 37.0 33.0 40.0 70-71 35.21281593308356 37.0 35.0 37.0 30.0 40.0 72-73 35.01105870699283 37.0 33.0 37.0 27.0 40.0 74-75 34.78868421656348 37.0 33.0 37.0 27.0 37.0 76-77 34.60380882615978 37.0 33.0 37.0 27.0 37.0 78-79 34.431748108755286 37.0 33.0 37.0 27.0 37.0 80-81 34.27526754067463 37.0 33.0 37.0 27.0 37.0 82-83 34.16318880983543 37.0 33.0 37.0 27.0 37.0 84-85 34.060410751905316 37.0 33.0 37.0 27.0 37.0 86-87 33.953880840941814 37.0 33.0 37.0 27.0 37.0 88-89 33.865591227966135 37.0 33.0 37.0 27.0 37.0 90-91 33.77684002493427 37.0 33.0 37.0 27.0 37.0 92-93 33.694360819572324 37.0 33.0 37.0 27.0 37.0 94-95 33.61066111190708 37.0 33.0 37.0 27.0 37.0 96-97 33.51698043007256 37.0 33.0 37.0 27.0 37.0 98-99 33.45172174413564 37.0 33.0 37.0 27.0 37.0 100-101 31.625750261190955 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18862333040220136 2306 2 0.3143004622636312 2306 3 0.33346731365087834 2306 4 0.44044483155865777 2306 5 0.421169659594014 2306 6 0.4642928359315235 2306 7 0.47153806341663795 2306 8 0.471342324538675 2306 9 0.47783927162461737 2306 10-11 0.49236795514568854 2306 12-13 0.4508483443206259 2306 14-15 0.81005839789362 2306 16-17 0.8026715481015145 2306 18-19 0.8291831288986629 2306 20-21 0.8276098801123482 2306 22-23 0.8256784410302345 2306 24-25 0.8478245275296317 2306 26-27 0.8578138240732045 2306 28-29 0.8626473310348075 2306 30-31 0.8408035146746258 2306 32-33 0.8856598754721929 2306 34-35 0.8828922430937425 2306 36-37 0.8857467989218719 2306 38-39 0.873254845016298 2306 40-41 0.920056610332658 2306 42-43 0.8822782116473391 2306 44-45 0.9205189139478591 2306 46-47 0.9344474341691651 2306 48-49 0.9027319882223139 2306 50-51 0.8367480932570786 2306 52-53 0.90684802353816 2306 54-55 1.0028693054198499 2306 56-57 0.9775984314572028 2306 58-59 0.9870502067679752 2306 60-61 0.9188915815018746 2306 62-63 0.9281257114163708 2306 64-65 0.9148961162594915 2306 66-67 0.8563675514159499 2306 68-69 0.9292651505453122 2306 70-71 0.9260959384785892 2306 72-73 0.9294587225286008 2306 74-75 0.96356524166778 2306 76-77 0.916402862861105 2306 78-79 0.8936396688327335 2306 80-81 0.915665799203019 2306 82-83 0.9674313576106357 2306 84-85 0.984922247337316 2306 86-87 0.958538031235733 2306 88-89 0.9665307364096378 2306 90-91 0.9450410815023105 2306 92-93 0.991473813120912 2306 94-95 0.976637870702838 2306 96-97 0.9498096109718688 2306 98-99 0.9802103519324135 2306 100-101 0.7981925310496472 2307 1 0.19302449969958957 2307 2 0.25228908449890497 2307 3 0.2561031108857392 2307 4 0.3718674892615468 2307 5 0.40547423336644783 2307 6 0.3564986309822231 2307 7 0.35465576521956876 2307 8 0.34786514294176385 2307 9 0.33642500855285107 2307 10-11 0.3485745157729738 2307 12-13 0.3470937352228205 2307 14-15 0.6401325368192019 2307 16-17 0.5927955155823099 2307 18-19 0.577021462327707 2307 20-21 0.6211472851381217 2307 22-23 0.62838574510819 2307 24-25 0.6256968043104152 2307 26-27 0.6422335637186976 2307 28-29 0.6145537111346755 2307 30-31 0.6294983684918236 2307 32-33 0.6459009965539124 2307 34-35 0.6955468716823745 2307 36-37 0.6585999301809551 2307 38-39 0.6132441362750782 2307 40-41 0.6790969376407716 2307 42-43 0.6703613540771087 2307 44-45 0.6935792278426689 2307 46-47 0.658441700834743 2307 48-49 0.6735644630502335 2307 50-51 0.5699932363090099 2307 52-53 0.6396300266977022 2307 54-55 0.7680567116950954 2307 56-57 0.7656316078707661 2307 58-59 0.7226782179444271 2307 60-61 0.7168919306707906 2307 62-63 0.7294991183406907 2307 64-65 0.7115775958738979 2307 66-67 0.7080024480761296 2307 68-69 0.7116937633423319 2307 70-71 0.720221690028751 2307 72-73 0.6621360831391598 2307 74-75 0.6775916547807057 2307 76-77 0.6373118037399479 2307 78-79 0.6837064768729704 2307 80-81 0.7191377262462098 2307 82-83 0.676067505576242 2307 84-85 0.7127563859537318 2307 86-87 0.7020530140023453 2307 88-89 0.7028190658980762 2307 90-91 0.6634028468329234 2307 92-93 0.7141709031369672 2307 94-95 0.6968338566258794 2307 96-97 0.7624597668223885 2307 98-99 0.699932127498208 2307 100-101 0.6739088899919317 2201 1 0.269757940050404 2201 2 0.3202341998796925 2201 3 0.34135304824592794 2201 4 0.43958565142119 2201 5 0.4422124329608579 2201 6 0.4572722314748958 2201 7 0.4009611354576563 2201 8 0.3699316983129748 2201 9 0.4269604168173373 2201 10-11 0.41222858232339377 2201 12-13 0.45259605793359015 2201 14-15 0.6981402683200528 2201 16-17 0.7228203536586548 2201 18-19 0.6848960024793911 2201 20-21 0.7394207510020792 2201 22-23 0.7522992609562351 2201 24-25 0.7761972260692573 2201 26-27 0.7568557945865848 2201 28-29 0.738764542880233 2201 30-31 0.783152170450407 2201 32-33 0.8014152003263746 2201 34-35 0.8118331667242913 2201 36-37 0.7693919519717696 2201 38-39 0.7827771472065308 2201 40-41 0.7758349339238535 2201 42-43 0.817557076291962 2201 44-45 0.8151533220893654 2201 46-47 0.815857370194756 2201 48-49 0.8202937333655242 2201 50-51 0.7473104556137216 2201 52-53 0.833786176848271 2201 54-55 0.883873253047156 2201 56-57 0.9053618498993572 2201 58-59 0.8032457523592669 2201 60-61 0.8536046010559559 2201 62-63 0.8619258024910508 2201 64-65 0.9041432745390381 2201 66-67 0.8801270952174818 2201 68-69 0.838284479321743 2201 70-71 0.8480466026573694 2201 72-73 0.8850719137600507 2201 74-75 0.8802364700018472 2201 76-77 0.8658675214421194 2201 78-79 0.8938122188603401 2201 80-81 0.9325237385669496 2201 82-83 0.9140439824019921 2201 84-85 0.9393573383802618 2201 86-87 0.8969493730482156 2201 88-89 0.864324991723386 2201 90-91 0.8390955628843599 2201 92-93 0.9449182290053528 2201 94-95 0.9329323370432903 2201 96-97 0.9356259987024913 2201 98-99 0.9391541553634255 2201 100-101 0.8616964945436116 1101 1 0.2681700706957528 1101 2 0.29592917510664307 1101 3 0.27284084980436063 1101 4 0.37048578178377056 1101 5 0.3710171179531301 1101 6 0.4242052050196534 1101 7 0.3867826172472846 1101 8 0.3999307339081497 1101 9 0.3695399534264183 1101 10-11 0.40915397817318677 1101 12-13 0.38463219080778543 1101 14-15 0.6205361793715483 1101 16-17 0.6503360548249475 1101 18-19 0.6367767758137077 1101 20-21 0.6275825618184783 1101 22-23 0.655942831966577 1101 24-25 0.6741604246690045 1101 26-27 0.6828989511427537 1101 28-29 0.6549716578527978 1101 30-31 0.7076094408392919 1101 32-33 0.7445192700027832 1101 34-35 0.7315836017860207 1101 36-37 0.6841274259639434 1101 38-39 0.6496108014483752 1101 40-41 0.7539436800840278 1101 42-43 0.7532664200312738 1101 44-45 0.6849791342645659 1101 46-47 0.7426013715727464 1101 48-49 0.7563813740938627 1101 50-51 0.7016768024594384 1101 52-53 0.7262006410973996 1101 54-55 0.7718945094571268 1101 56-57 0.7393283437888201 1101 58-59 0.7724181082947013 1101 60-61 0.7947700411052381 1101 62-63 0.7393637115334428 1101 64-65 0.7800683834664 1101 66-67 0.7733520793479798 1101 68-69 0.7264852341804371 1101 70-71 0.7730869821114794 1101 72-73 0.7485679516235706 1101 74-75 0.7057455573981883 1101 76-77 0.782165487187676 1101 78-79 0.8417828903979796 1101 80-81 0.8015000748234087 1101 82-83 0.7936888111369598 1101 84-85 0.7794035370498946 1101 86-87 0.7769575169111818 1101 88-89 0.8247000484401426 1101 90-91 0.73900764690881 1101 92-93 0.7336567834952632 1101 94-95 0.7496528216575129 1101 96-97 0.778397520643658 1101 98-99 0.8002741508340776 1101 100-101 0.8285996882909394 2304 1 0.17431042478526848 2304 2 0.2653754281019687 2304 3 0.27399109951780787 2304 4 0.3723686372523076 2304 5 0.397087307176335 2304 6 0.39822121021942536 2304 7 0.3433959931466859 2304 8 0.38610707657513643 2304 9 0.3579942185599805 2304 10-11 0.40857979011980206 2304 12-13 0.41315420300343675 2304 14-15 0.6964088776776904 2304 16-17 0.6937737517399469 2304 18-19 0.6685055254307883 2304 20-21 0.6942060901110949 2304 22-23 0.7208687789406056 2304 24-25 0.7199721132323091 2304 26-27 0.724773864222314 2304 28-29 0.7656879513069583 2304 30-31 0.8120056630010097 2304 32-33 0.8096086721255347 2304 34-35 0.814461864448468 2304 36-37 0.82024233988151 2304 38-39 0.7839465299607227 2304 40-41 0.7849924382277251 2304 42-43 0.7839969333083658 2304 44-45 0.7945356396440744 2304 46-47 0.7947742187710105 2304 48-49 0.8075641947558907 2304 50-51 0.7451021122794614 2304 52-53 0.8747369412471429 2304 54-55 0.8948122788193871 2304 56-57 0.8275583978193737 2304 58-59 0.8297438848045928 2304 60-61 0.8518694561226567 2304 62-63 0.8972192290368071 2304 64-65 0.8879420579216202 2304 66-67 0.9085311139619279 2304 68-69 0.8702432752531237 2304 70-71 0.8959743223982244 2304 72-73 0.8944936065704567 2304 74-75 0.8931134670637846 2304 76-77 0.9200957300862953 2304 78-79 0.9268037287364592 2304 80-81 0.9485763925288424 2304 82-83 0.9497553025419236 2304 84-85 0.9650596557936311 2304 86-87 1.0080402299174764 2304 88-89 1.0349346418789978 2304 90-91 1.0274564785830478 2304 92-93 0.9934871122306248 2304 94-95 1.024751882222617 2304 96-97 1.053117448383766 2304 98-99 1.0336110426006755 2304 100-101 1.1517993886160731 2202 1 0.21262789884224276 2202 2 0.22510558169060602 2202 3 0.24851431992743755 2202 4 0.2540807169820596 2202 5 0.300889368440842 2202 6 0.2891338622216466 2202 7 0.2638439613018875 2202 8 0.2789638773845624 2202 9 0.25500142634786727 2202 10-11 0.2873418019174423 2202 12-13 0.3013494983365703 2202 14-15 0.46871824141609153 2202 16-17 0.45338060108773703 2202 18-19 0.4500873274669104 2202 20-21 0.46410161232052616 2202 22-23 0.428601672713107 2202 24-25 0.4513780609768929 2202 26-27 0.423975126639057 2202 28-29 0.48522455168708234 2202 30-31 0.5065520772701646 2202 32-33 0.5286689548597252 2202 34-35 0.5363328036670012 2202 36-37 0.4873605131329839 2202 38-39 0.5415202800928043 2202 40-41 0.491042896501817 2202 42-43 0.4773380686420765 2202 44-45 0.49425996502768754 2202 46-47 0.49218728813625745 2202 48-49 0.47485405795797675 2202 50-51 0.5203726875044978 2202 52-53 0.5757958432031174 2202 54-55 0.5721606023686405 2202 56-57 0.5679375497187564 2202 58-59 0.5475030756375077 2202 60-61 0.562388317735433 2202 62-63 0.5904023347255034 2202 64-65 0.5878966066212001 2202 66-67 0.5465100587143397 2202 68-69 0.5913031005219054 2202 70-71 0.5139112693079682 2202 72-73 0.5944726026804332 2202 74-75 0.5965006518133436 2202 76-77 0.5911822725220972 2202 78-79 0.5848001339085869 2202 80-81 0.6148558467796974 2202 82-83 0.6315980034532203 2202 84-85 0.6270255247027734 2202 86-87 0.5869655043301165 2202 88-89 0.6169588001834612 2202 90-91 0.640056977062514 2202 92-93 0.6214765030482496 2202 94-95 0.5867301865464896 2202 96-97 0.6055568340361077 2202 98-99 0.6226571020547311 2202 100-101 0.6027398844544898 1102 1 0.11922426572952105 1102 2 0.16345540455959195 1102 3 0.17605283346092193 1102 4 0.18083429468229895 1102 5 0.17524331799525328 1102 6 0.21419190779447206 1102 7 0.19215359499334284 1102 8 0.21393216908501955 1102 9 0.21833595437211528 1102 10-11 0.17897155045019275 1102 12-13 0.2051153469844067 1102 14-15 0.3232134474092092 1102 16-17 0.3456302165226077 1102 18-19 0.2876855922007877 1102 20-21 0.31137950955117333 1102 22-23 0.3301380742164639 1102 24-25 0.3872280036448146 1102 26-27 0.33543949942994544 1102 28-29 0.36548312618991474 1102 30-31 0.30707562052725024 1102 32-33 0.3829936332429682 1102 34-35 0.3482608597195096 1102 36-37 0.4416821082684663 1102 38-39 0.40217765576922204 1102 40-41 0.37885678256078137 1102 42-43 0.40047237200560204 1102 44-45 0.41177985373315096 1102 46-47 0.340599625340694 1102 48-49 0.3636426899250509 1102 50-51 0.3984313631045566 1102 52-53 0.4320113008381341 1102 54-55 0.5226738484866189 1102 56-57 0.5247350436385361 1102 58-59 0.47433635437323574 1102 60-61 0.49798471707737946 1102 62-63 0.48054069598266835 1102 64-65 0.49774738424257237 1102 66-67 0.4778233824293707 1102 68-69 0.43693415574176697 1102 70-71 0.4597501587867967 1102 72-73 0.46521834395662154 1102 74-75 0.4699282346559954 1102 76-77 0.44909015037803357 1102 78-79 0.44845432691845133 1102 80-81 0.5204651039461439 1102 82-83 0.5441326179381889 1102 84-85 0.48588635160300697 1102 86-87 0.4495531272089224 1102 88-89 0.46255205831128876 1102 90-91 0.5084736225947069 1102 92-93 0.4139117822332068 1102 94-95 0.4807193009407271 1102 96-97 0.5052436897247858 1102 98-99 0.5584247941408265 1102 100-101 0.6205060344624904 2305 1 0.17945845745668265 2305 2 0.16545348792210746 2305 3 0.19004271413185592 2305 4 0.20437123318178152 2305 5 0.24148167692857214 2305 6 0.27066136995147616 2305 7 0.221357729559422 2305 8 0.22023079447156846 2305 9 0.2004550808994665 2305 10-11 0.23680460612821008 2305 12-13 0.2662458728898258 2305 14-15 0.3945948636589307 2305 16-17 0.35580892227978467 2305 18-19 0.3198904447086477 2305 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1118.0 5 957.0 6 781.0 7 1278.0 8 2495.0 9 7991.0 10 24484.0 11 28069.0 12 21154.0 13 22095.0 14 24875.0 15 28522.0 16 33454.0 17 38740.0 18 44382.0 19 50927.0 20 58008.0 21 65573.0 22 74110.0 23 85280.0 24 97804.0 25 113798.0 26 134800.0 27 165255.0 28 187450.0 29 222315.0 30 276595.0 31 351049.0 32 460141.0 33 617028.0 34 855128.0 35 1333152.0 36 2763265.0 37 6675992.0 38 5902031.0 39 5079.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.518699419313474 12.537376646356005 10.813985540267227 55.129938394063295 2 22.14483208824651 19.083719682325967 40.25122576095084 18.520222468476685 3 24.466084827127382 23.727304029170377 23.84378489750786 27.962826246194382 4 27.722426537230447 28.52947068597862 17.316806293468225 26.431296483322708 5 28.805368310797935 29.876716991491588 21.200993034098687 20.11692166361179 6 20.947717390985172 32.550808792632616 23.171932632441177 23.329541183941036 7 19.211666804432056 14.355468115319885 41.665785273070824 24.76707980717724 8 21.148161232151335 19.55770392784147 27.801880672404096 31.492254167603107 9 22.308335469954294 19.232395747943727 30.787217830613507 27.672050951488476 10-11 25.443095661662973 28.11262898479951 20.988592140096802 25.45568321344071 12-13 23.33572320592114 22.26209274045336 27.643808884285363 26.75837516934013 14-15 24.280748829524974 23.762524164053474 25.953531578596966 26.003195427824586 16-17 25.44161525039111 24.061396335089675 24.546355166272633 25.950633248246586 18-19 25.01984814456956 24.28558416890561 24.636575206175724 26.0579924803491 20-21 24.90963557038683 24.594020447656632 24.96467172795764 25.5316722539989 22-23 25.08480027923955 24.520840798831994 24.711647747863022 25.682711174065435 24-25 24.85018777938245 24.722091111896262 24.699587773407558 25.72813333531373 26-27 24.650089434241156 24.94110339946412 24.971917889014875 25.43688927727985 28-29 25.022403761554795 24.497832343244617 24.657015856271418 25.822748038929166 30-31 24.89342121661385 24.422163034117197 24.809311550856986 25.875104198411975 32-33 25.054513071846806 24.583446658991097 24.87610971878434 25.485930550377763 34-35 25.25318669037348 24.671981501212905 24.44580784403201 25.62902396438161 36-37 24.849305156282117 24.743173922859707 24.539296699777026 25.868224221081153 38-39 24.99910861516402 24.658971569891566 24.917394927877034 25.424524887067378 40-41 25.29857248825763 24.49832293872748 24.53680872427315 25.66629584874174 42-43 24.985078859940632 24.340254479170955 24.853435762535863 25.82123089835255 44-45 25.276530377358487 24.56047046608887 24.819001176608214 25.343997979944426 46-47 25.323862688899684 24.403943573452576 24.570668332999222 25.70152540464851 48-49 25.01760730812923 24.32017126693941 24.583433787406214 26.078787637525146 50-51 25.1076604686957 24.57958000433601 24.774404217266966 25.538355309701327 52-53 25.285956024361518 24.473533173411592 24.652072014749653 25.588438787477237 54-55 24.820973842412887 24.414754068925102 24.789861881281514 25.9744102073805 56-57 24.886793549166356 24.63721775292049 25.1003360364814 25.37565266143175 58-59 25.175945215644045 24.609870916824796 24.620658877720388 25.593524989810767 60-61 24.8456668512485 24.794393763736828 24.63242693753517 25.727512447479505 62-63 24.985101934631807 24.95668845218168 24.77320657220263 25.285003040983884 64-65 25.100015435134544 24.922150303931566 24.464604001175882 25.513230259758004 66-67 24.975085079989526 24.772378816936737 24.571733100395292 25.68080300267845 68-69 25.04084361112429 24.93919284103588 24.72757947464685 25.292384073192984 70-71 25.305108532380093 24.766060951755005 24.424829681646663 25.50400083421824 72-73 25.075542430686237 24.73476563588825 24.546260390318725 25.643431543106786 74-75 25.14448976855583 24.90655463834443 24.677909503501517 25.271046089598226 76-77 25.302339399914224 24.774019594750506 24.449791001707037 25.47385000362823 78-79 25.20907262707391 24.69180443201264 24.5339201169958 25.565202823917648 80-81 25.245644344822455 24.904020979075696 24.5472101793752 25.303124496726646 82-83 25.43298041915208 24.810541992538116 24.28548797748084 25.470989610828966 84-85 25.135111303327612 24.748099599912095 24.57063929497714 25.54614980178315 86-87 25.290973322205346 25.009961838790773 24.561303538142766 25.13776130086111 88-89 25.416483158957377 24.752205914125973 24.275321385742693 25.555989541173957 90-91 25.359836848555737 24.634494893982158 24.377987285432862 25.62768097202924 92-93 25.35429409405455 24.92479345913115 24.51631411572143 25.204598331092875 94-95 25.559431740153343 24.75696741393321 24.333697780214965 25.349903065698477 96-97 25.266603908582713 24.800710828215102 24.440133476950944 25.492551786251244 98-99 25.46485593798224 24.89003174999271 24.471545877771913 25.173566434253136 100-101 25.838050161756797 24.74143524210084 24.07785748576948 25.342657110372883 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13523.0 1 7376.0 2 902.5 3 535.5 4 470.0 5 436.5 6 420.0 7 415.5 8 447.5 9 456.0 10 454.5 11 491.0 12 499.0 13 548.5 14 660.0 15 743.5 16 838.5 17 941.0 18 1113.0 19 1397.5 20 1886.5 21 2637.5 22 3709.5 23 5438.0 24 7988.5 25 11871.0 26 17601.0 27 25167.5 28 35199.5 29 48252.5 30 64221.5 31 83873.5 32 108457.0 33 138634.5 34 175625.0 35 222061.5 36 278726.5 37 346735.5 38 427140.5 39 512503.0 40 597297.0 41 676892.0 42 747930.0 43 795376.0 44 820420.0 45 823778.5 46 804030.0 47 773114.5 48 734935.5 49 697504.0 50 659753.0 51 623450.0 52 596876.5 53 582736.5 54 579686.0 55 577342.5 56 571074.5 57 566268.5 58 549720.5 59 529057.0 60 512521.0 61 490483.0 62 476554.5 63 453394.5 64 405375.0 65 368323.0 66 348632.5 67 326837.0 68 299770.5 69 262085.0 70 221849.5 71 183910.5 72 150456.5 73 123957.0 74 94505.5 75 68363.0 76 49474.0 77 34768.0 78 23843.5 79 16221.5 80 10518.0 81 6467.0 82 4056.0 83 2469.0 84 1566.5 85 1125.5 86 842.0 87 642.5 88 487.5 89 389.0 90 313.5 91 272.0 92 234.5 93 192.0 94 158.5 95 114.0 96 69.5 97 50.5 98 38.5 99 24.5 100 17.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09263543706338018 2 0.0817660072433688 3 0.08672458341347133 4 0.1047120469209238 5 0.08825399875982691 6 0.08964874860398767 7 0.09729582533576558 8 0.08497393188493853 9 0.11294588048369347 10-11 0.08544766588373105 12-13 0.07885386575840557 14-15 0.12817991541948376 16-17 0.12882198129601982 18-19 0.12122059464534374 20-21 0.1333573230306529 22-23 0.13126038878219054 24-25 0.10584467998402676 26-27 0.1096994799843538 28-29 0.09924607037648002 30-31 0.08748448160442787 32-33 0.10806906551135208 34-35 0.1267635229053274 36-37 0.11365768385243759 38-39 0.1154876918376209 40-41 0.14451532178393892 42-43 0.11900582808246087 44-45 0.11789724243046415 46-47 0.13512961722918132 48-49 0.14564795484704188 50-51 0.11690167961066664 52-53 0.08824437979538442 54-55 0.09088959501706861 56-57 0.09528546176728561 58-59 0.09187313413131301 60-61 0.09223625003901692 62-63 0.08133074910234621 64-65 0.10323553587900189 66-67 0.10133819514272113 68-69 0.09931821260979867 70-71 0.09963323369529015 72-73 0.096949542615836 74-75 0.13459816944373384 76-77 0.15122695422368487 78-79 0.14992117980061714 80-81 0.12067231367212192 82-83 0.10084281847393302 84-85 0.10435854997766236 86-87 0.11062290057083264 88-89 0.10697009882379785 90-91 0.11104853974741272 92-93 0.09841162521109419 94-95 0.094672252784077 96-97 0.09827215022667811 98-99 0.08696024804231228 100-101 0.09408068647086398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.0792259E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.162834384971575 #Duplication Level Percentage of deduplicated Percentage of total 1 74.82101229016315 38.2805506031754 2 13.12224712431517 13.427427127600133 3 4.479865754214737 6.876078889493832 4 2.40385422306338 4.919519820008249 5 1.303299480770592 3.3340247744342624 6 0.795538590788404 2.442120550041648 7 0.5643131416498826 2.021030186524716 8 0.4544814797286725 1.8602048542715914 9 0.2936183674913424 1.3520113117510748 >10 1.5964142316543084 14.549603823939123 >50 0.103362213744247 3.6664884608853745 >100 0.05827744026159815 5.292573855083462 >500 0.0029292888659307186 1.0359597001156085 >1k 7.26941778242663E-4 0.5839343338980725 >5k 4.058231903480474E-5 0.14484563364603112 >10k+ 1.88491913170035E-5 0.21362607513142096 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 30458 0.14648720949464897 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.15630336270820788 1.9237928884975894E-5 0.0 2 0.1565342178548276 1.9237928884975894E-5 9.618964442487947E-6 3 0.15811653750561688 2.404741110621987E-5 9.618964442487947E-6 4 0.1636762989533749 2.404741110621987E-5 1.442844666373192E-5 5 0.1645949100576325 2.404741110621987E-5 1.442844666373192E-5 6 0.16727860113708665 5.771378665492768E-5 2.404741110621987E-5 7 0.167798025216981 5.771378665492768E-5 2.404741110621987E-5 8 0.16919758454336298 6.733275109741563E-5 2.885689332746384E-5 9 0.171385898954029 7.21422333186596E-5 2.885689332746384E-5 10-11 0.17553648211096257 9.618964442487948E-5 3.847585776995179E-5 12-13 0.17953797131903754 1.0580860886736741E-4 4.569008110181775E-5 14-15 0.1836356501715374 1.1542757330985536E-4 5.5309045544305694E-5 16-17 0.1879377320184401 1.2264179664172133E-4 5.771378665492768E-5 18-19 0.1921340052564755 1.250465377523433E-4 6.011852776554967E-5 20-21 0.19563049883131986 1.442844666373192E-4 6.492800998679363E-5 22-23 0.19862440151404426 1.7073661885416106E-4 7.21422333186596E-5 24-25 0.201094070634653 1.8997454773913696E-4 8.657067998239153E-5 26-27 0.2034002173597395 2.1161721773473483E-4 9.378490331425748E-5 28-29 0.20540817618710885 2.2845040550908876E-4 1.0340386775674543E-4 30-31 0.2071852798678585 2.4047411106219868E-4 1.2023705553109934E-4 32-33 0.20871709995532473 2.4768833439406466E-4 1.3466550219483125E-4 34-35 0.21014551617503419 2.573072988365526E-4 1.5630817219042915E-4 36-37 0.21160038454696048 2.789499688321505E-4 1.5871291330105111E-4 38-39 0.21321637057329845 2.813547099427725E-4 1.6833187774353906E-4 40-41 0.21463997731078666 2.9578315660650433E-4 1.8756980662851496E-4 42-43 0.21606839353049612 3.174258266021023E-4 1.9959351218162488E-4 44-45 0.21748959552687375 3.60711166593298E-4 2.2123618217722278E-4 46-47 0.2189973682032337 3.6792538992516395E-4 2.2123618217722278E-4 48-49 0.2206229731940142 3.799490954782739E-4 2.3566462884095472E-4 50-51 0.22234957731144075 4.0880598880573774E-4 2.4528359328344267E-4 52-53 0.22386937369335386 4.3285339991195763E-4 2.500930755046866E-4 54-55 0.22548295497858123 4.3766288213320157E-4 2.597120399471746E-4 56-57 0.2271566547915741 4.4006762324382356E-4 2.693310043896625E-4 58-59 0.22888085416789006 4.4487710546506755E-4 2.693310043896625E-4 60-61 0.23081426602083016 4.569008110181775E-4 2.693310043896625E-4 62-63 0.23308915111147854 4.8816244545626334E-4 2.717357455002845E-4 64-65 0.23552755859764923 5.001861510093733E-4 2.741404866109065E-4 66-67 0.23810784580934663 5.122098565624832E-4 2.8375945105339447E-4 68-69 0.240904559721 5.194240798943492E-4 2.957831566065044E-4 70-71 0.24426879253476017 5.43471491000569E-4 3.029973799383703E-4 72-73 0.24854442222944606 5.45876232111191E-4 3.102116032702363E-4 74-75 0.25363044967841153 5.699236432174109E-4 3.126163443808583E-4 76-77 0.259798610627157 5.987805365448746E-4 3.246400499339682E-4 78-79 0.26726533177563827 6.035900187661186E-4 3.3425901437645613E-4 80-81 0.2773796728869143 6.348516532042046E-4 3.534969432614321E-4 82-83 0.29087267525861427 6.492800998679364E-4 3.871633188101399E-4 84-85 0.3088986146238367 6.540895820891804E-4 4.0880598880573774E-4 86-87 0.33169796509364374 6.637085465316684E-4 4.1361547102698173E-4 88-89 0.3604298118833552 6.757322520847783E-4 4.3044865880133564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGT 14300 0.0 21.393188 9 GCCGTAT 8325 0.0 18.404032 48-49 AAGAGCG 17860 0.0 17.284513 8 GAGTGTA 8785 0.0 17.138863 28-29 CCGTATC 9860 0.0 16.454372 48-49 ATCGGAA 20620 0.0 16.099241 3 TTAAAAA 19155 0.0 15.716736 56-57 GTAGGGA 10565 0.0 15.514005 18-19 CGCCGTA 10450 0.0 15.435522 46-47 TCGTGTA 10415 0.0 15.419209 14-15 TAAAAAA 22035 0.0 15.183606 58-59 CGTATCA 10010 0.0 15.134817 50-51 AGTGTAG 10465 0.0 15.068268 28-29 CGTGTAG 10380 0.0 14.875365 16-17 CGAATTA 7515 0.0 14.857424 14-15 GTCGTGT 10825 0.0 14.220726 14-15 TGTAGGG 10825 0.0 14.175843 18-19 CGCGTAT 1595 0.0 13.993349 1 GATCGGA 24575 0.0 13.661877 2 TCGGAAG 23740 0.0 13.52347 4 >>END_MODULE