FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n02 k12-3.3420000000cc5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n02 k12-3.3420000000cc5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20792259
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC304580.14648720949464897Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT143000.021.3931889
GCCGTAT83250.018.40403248-49
AAGAGCG178600.017.2845138
GAGTGTA87850.017.13886328-29
CCGTATC98600.016.45437248-49
ATCGGAA206200.016.0992413
TTAAAAA191550.015.71673656-57
GTAGGGA105650.015.51400518-19
CGCCGTA104500.015.43552246-47
TCGTGTA104150.015.41920914-15
TAAAAAA220350.015.18360658-59
CGTATCA100100.015.13481750-51
AGTGTAG104650.015.06826828-29
CGTGTAG103800.014.87536516-17
CGAATTA75150.014.85742414-15
GTCGTGT108250.014.22072614-15
TGTAGGG108250.014.17584318-19
CGCGTAT15950.013.9933491
GATCGGA245750.013.6618772
TCGGAAG237400.013.523474
Produced by FastQC (version 0.11.2)