FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 mx24-2.3410000000ce26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 mx24-2.3410000000ce26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19536313
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA45950.013.0190673
CCGGGAT89350.011.4351361
CGGAAAT61950.011.1997661
CTCGAAT86650.011.0236371
GTCCGAT69700.010.9089921
CTCGTTT82250.010.6311261
CGATTAT52000.010.5910074
CTCAGAT150450.010.5183941
TGCACAC105750.010.370747
CTGGAAT172150.010.2967271
GTGCACA98650.010.2027436
CGAATTA54700.010.19944414-15
GTCCAAT99800.010.1425341
CTCGGAT108700.010.0115961
CGCAAAT48500.09.8963961
CTCGACT94850.09.8201061
CGCGAAT23850.09.763491
CTCGGTT82350.09.6948951
CGCGTAT18650.09.4280161
CTCGATT108150.09.3594531