##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l08n01 mx12-3.3410000000cdba.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17839626
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.780192308964324	33.0	33.0	33.0	33.0	33.0
2	32.77489600959123	33.0	33.0	33.0	33.0	33.0
3	32.623241316830295	33.0	33.0	33.0	33.0	33.0
4	36.6169328325605	37.0	37.0	37.0	37.0	37.0
5	36.66867068849986	37.0	37.0	37.0	37.0	37.0
6	36.60965981013279	37.0	37.0	37.0	37.0	37.0
7	36.63899557087127	37.0	37.0	37.0	37.0	37.0
8	36.63444099108356	37.0	37.0	37.0	37.0	37.0
9	36.63439474571945	37.0	37.0	37.0	37.0	37.0
10-11	36.60906374943062	37.0	37.0	37.0	37.0	37.0
12-13	36.60612764527687	37.0	37.0	37.0	37.0	37.0
14-15	38.912987105222946	40.0	40.0	40.0	37.0	40.0
16-17	38.89680873915182	40.0	40.0	40.0	37.0	40.0
18-19	38.870706398217095	40.0	40.0	40.0	37.0	40.0
20-21	38.84819216501512	40.0	40.0	40.0	37.0	40.0
22-23	38.81313660947825	40.0	40.0	40.0	37.0	40.0
24-25	38.7823429426155	40.0	38.5	40.0	37.0	40.0
26-27	38.71458843924194	40.0	37.0	40.0	37.0	40.0
28-29	38.66709885061492	40.0	37.0	40.0	37.0	40.0
30-31	38.60248320228238	40.0	37.0	40.0	37.0	40.0
32-33	38.50561659756768	40.0	37.0	40.0	37.0	40.0
34-35	38.42128882074097	40.0	37.0	40.0	37.0	40.0
36-37	38.338133461990736	40.0	37.0	40.0	37.0	40.0
38-39	38.22140416508732	40.0	37.0	40.0	37.0	40.0
40-41	38.09489655220351	40.0	37.0	40.0	37.0	40.0
42-43	37.967898878597566	40.0	37.0	40.0	33.0	40.0
44-45	37.82675258999264	40.0	37.0	40.0	33.0	40.0
46-47	37.697840078037515	40.0	37.0	40.0	33.0	40.0
48-49	37.530664263925715	40.0	37.0	40.0	33.0	40.0
50-51	37.36835211679886	40.0	37.0	40.0	33.0	40.0
52-53	37.17097799583915	37.0	37.0	40.0	33.0	40.0
54-55	36.96785669722	37.0	37.0	40.0	33.0	40.0
56-57	36.77268096876022	37.0	37.0	40.0	33.0	40.0
58-59	36.60013169558599	37.0	37.0	40.0	33.0	40.0
60-61	36.38529905279404	37.0	37.0	40.0	33.0	40.0
62-63	36.1667531595113	37.0	37.0	40.0	33.0	40.0
64-65	35.98394475870738	37.0	37.0	38.5	33.0	40.0
66-67	35.73382233461621	37.0	37.0	37.0	33.0	40.0
68-69	35.54545344728639	37.0	35.0	37.0	33.0	40.0
70-71	35.34766023121785	37.0	33.0	37.0	33.0	40.0
72-73	35.086333003842114	37.0	33.0	37.0	33.0	38.5
74-75	34.84972927683574	37.0	33.0	37.0	30.0	37.0
76-77	32.052049157308566	33.0	30.0	35.0	24.5	37.0
78-79	34.305449901247925	37.0	33.0	37.0	27.0	37.0
80-81	34.56958680075468	37.0	33.0	37.0	27.0	37.0
82-83	34.54771630302115	37.0	33.0	37.0	27.0	37.0
84-85	34.44193151807106	37.0	33.0	37.0	27.0	37.0
86-87	34.36590464396507	37.0	33.0	37.0	27.0	37.0
88-89	34.27273685558206	37.0	33.0	37.0	27.0	37.0
90-91	34.19272632172894	37.0	33.0	37.0	27.0	37.0
92-93	34.10379118934444	37.0	33.0	37.0	27.0	37.0
94-95	34.01889167967983	37.0	33.0	37.0	27.0	37.0
96-97	33.94906067537515	37.0	33.0	37.0	27.0	37.0
98-99	33.88530748906956	37.0	33.0	37.0	27.0	37.0
100-101	31.889904446427295	35.0	30.0	37.0	21.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.03320390063866796
2306	2	0.07776622966665059
2306	3	0.2241859612095638
2306	4	0.20644578504787603
2306	5	0.17962989147467567
2306	6	0.19362449160263395
2306	7	0.18008161207111328
2306	8	0.18971278117585655
2306	9	0.21076809402250518
2306	10-11	0.22100443623484267
2306	12-13	0.20838234555498758
2306	14-15	0.4032942079468924
2306	16-17	0.44198843356483053
2306	18-19	0.4535874923450862
2306	20-21	0.414140414113902
2306	22-23	0.4683156297619817
2306	24-25	0.4536364941990243
2306	26-27	0.45115594600232356
2306	28-29	0.47163513865994133
2306	30-31	0.47647816220312933
2306	32-33	0.46483830266372905
2306	34-35	0.45404701922002033
2306	36-37	0.44795242637678
2306	38-39	0.4765394379493486
2306	40-41	0.50223989559845
2306	42-43	0.4926425638585812
2306	44-45	0.45178471540685905
2306	46-47	0.4827251603041489
2306	48-49	0.4717720488073951
2306	50-51	0.4731855337046369
2306	52-53	0.48509240403419085
2306	54-55	0.5090490206958975
2306	56-57	0.476583092939336
2306	58-59	0.492461784735319
2306	60-61	0.5276571738474303
2306	62-63	0.5338115213082233
2306	64-65	0.5602417237039532
2306	66-67	0.5158326661841599
2306	68-69	0.5531704526474641
2306	70-71	0.5148861126502666
2306	72-73	0.4390624010624151
2306	74-75	0.472345352609139
2306	76-77	0.2814201726197325
2306	78-79	0.4981015518973706
2306	80-81	0.5539043784455302
2306	82-83	0.5861477562055839
2306	84-85	0.5604891363151907
2306	86-87	0.5704214796441249
2306	88-89	0.553141870939946
2306	90-91	0.564555069757823
2306	92-93	0.5899391730733328
2306	94-95	0.6201370026769837
2306	96-97	0.6087629861146908
2306	98-99	0.5425867977707597
2306	100-101	0.31926827556531734
2307	1	0.07770965670518848
2307	2	0.0970925364240216
2307	3	0.23115536969579864
2307	4	0.19413809682239958
2307	5	0.18952018281565586
2307	6	0.18889350284803896
2307	7	0.15576127910978954
2307	8	0.1778326350299153
2307	9	0.19180758916403562
2307	10-11	0.19628646009044104
2307	12-13	0.1952072554841351
2307	14-15	0.44303625383021483
2307	16-17	0.43771458228815163
2307	18-19	0.420280420845053
2307	20-21	0.43107989057902785
2307	22-23	0.45545401852120193
2307	24-25	0.4626884241250977
2307	26-27	0.4901318066496003
2307	28-29	0.4718130492338375
2307	30-31	0.44300753558834316
2307	32-33	0.4785743785837582
2307	34-35	0.4454894659861566
2307	36-37	0.47448141024387525
2307	38-39	0.4667386308491075
2307	40-41	0.4535207927651328
2307	42-43	0.4231602556200684
2307	44-45	0.4370515615836794
2307	46-47	0.4346421287447342
2307	48-49	0.4312161858323691
2307	50-51	0.44165820095380326
2307	52-53	0.44577641308980986
2307	54-55	0.45198463985651927
2307	56-57	0.4644299025788001
2307	58-59	0.45246412843420813
2307	60-61	0.4688788873278611
2307	62-63	0.42346415925889147
2307	64-65	0.4562460301509219
2307	66-67	0.4433892919452376
2307	68-69	0.4407600537349694
2307	70-71	0.4293762139390225
2307	72-73	0.40595205268148504
2307	74-75	0.4394400735444748
2307	76-77	0.39812790922453445
2307	78-79	0.5335395874663362
2307	80-81	0.47580993067580124
2307	82-83	0.4145296426702174
2307	84-85	0.43339124703333454
2307	86-87	0.47520562131799693
2307	88-89	0.43300010559984514
2307	90-91	0.4163881530636502
2307	92-93	0.46342831844719967
2307	94-95	0.4331665558804616
2307	96-97	0.36382079894347896
2307	98-99	0.3581521164452184
2307	100-101	0.23775410249118423
2201	1	0.05270681971347102
2201	2	0.07758722438663312
2201	3	0.21939809478642758
2201	4	0.2104837340375525
2201	5	0.16072559653213148
2201	6	0.18348734600016314
2201	7	0.15808722480456794
2201	8	0.14565864560232455
2201	9	0.16838949163851424
2201	10-11	0.160281683186966
2201	12-13	0.18546233037444182
2201	14-15	0.3874514017678834
2201	16-17	0.3660100254354006
2201	18-19	0.36798799823176154
2201	20-21	0.38249842441457815
2201	22-23	0.3920684583959613
2201	24-25	0.3771404986042768
2201	26-27	0.3824270571867743
2201	28-29	0.43331920639327137
2201	30-31	0.40591863335897216
2201	32-33	0.4075581206410135
2201	34-35	0.3949379317002695
2201	36-37	0.3702707487631258
2201	38-39	0.420948239841195
2201	40-41	0.3898860299853055
2201	42-43	0.38704587806535784
2201	44-45	0.35128449676266627
2201	46-47	0.3839793352466856
2201	48-49	0.43599807662251067
2201	50-51	0.4574443799919763
2201	52-53	0.44040717859829925
2201	54-55	0.45260516252673
2201	56-57	0.40965095999695933
2201	58-59	0.3922199950858456
2201	60-61	0.37700996135193066
2201	62-63	0.44185744107029734
2201	64-65	0.36678593141446925
2201	66-67	0.3784960301838254
2201	68-69	0.3457953889105241
2201	70-71	0.368484482300822
2201	72-73	0.39992896542035794
2201	74-75	0.34043162016943995
2201	76-77	0.3054341746988527
2201	78-79	0.5010237756067255
2201	80-81	0.46634364380201987
2201	82-83	0.5081315206820065
2201	84-85	0.5485933661657825
2201	86-87	0.4977599457373856
2201	88-89	0.5145771184972006
2201	90-91	0.5207486236727235
2201	92-93	0.5732825473722158
2201	94-95	0.5600923136866385
2201	96-97	0.5372878069657503
2201	98-99	0.5278867074802847
2201	100-101	0.4987082502722835
1101	1	0.03836576752718912
1101	2	0.07563604159803106
1101	3	0.21530823757006345
1101	4	0.2005006387998307
1101	5	0.16723992919447284
1101	6	0.15384783620550024
1101	7	0.14728447742298556
1101	8	0.15485112890857522
1101	9	0.1634025758447777
1101	10-11	0.13438657222891237
1101	12-13	0.16465427752218176
1101	14-15	0.3213841506160833
1101	16-17	0.3352509860992825
1101	18-19	0.3105092226402988
1101	20-21	0.32261499772522484
1101	22-23	0.35201227084146325
1101	24-25	0.3794560084014549
1101	26-27	0.37234607923038254
1101	28-29	0.38756247429997615
1101	30-31	0.3764026946513823
1101	32-33	0.36070304866756686
1101	34-35	0.3909804893874167
1101	36-37	0.37913422912730965
1101	38-39	0.41653275541973045
1101	40-41	0.45773222966187177
1101	42-43	0.44587050093239355
1101	44-45	0.3942687248280876
1101	46-47	0.4165074723726292
1101	48-49	0.4106795545074533
1101	50-51	0.44158302763229784
1101	52-53	0.45623123060056514
1101	54-55	0.44624240011250293
1101	56-57	0.468278626190866
1101	58-59	0.4186754476594601
1101	60-61	0.43163110904897906
1101	62-63	0.454212085386672
1101	64-65	0.5002684416018184
1101	66-67	0.4594573502320003
1101	68-69	0.3827320849065714
1101	70-71	0.4835970955204374
1101	72-73	0.5031675826060038
1101	74-75	0.47403408677708114
1101	76-77	0.3440076969391086
1101	78-79	0.5190977648250694
1101	80-81	0.40302014318150015
1101	82-83	0.4661874667398962
1101	84-85	0.5090962812050961
1101	86-87	0.5359392235429397
1101	88-89	0.478254335594535
1101	90-91	0.5378979579200518
1101	92-93	0.5315659341343562
1101	94-95	0.46448569846552346
1101	96-97	0.49542199229822614
1101	98-99	0.4286419420980323
1101	100-101	0.503444870018324
2304	1	0.05778927278614532
2304	2	0.08410359255280042
2304	3	0.22364331652302383
2304	4	0.17925982557810016
2304	5	0.167999149982478
2304	6	0.1901583987970099
2304	7	0.1809253630202292
2304	8	0.1617345919244073
2304	9	0.18319953305650927
2304	10-11	0.17897802531414442
2304	12-13	0.16811170359191152
2304	14-15	0.34276571260993194
2304	16-17	0.37072890705378114
2304	18-19	0.4158009295115406
2304	20-21	0.38565011603979826
2304	22-23	0.40334232526344493
2304	24-25	0.4099363324583365
2304	26-27	0.38880032043966395
2304	28-29	0.40101352907644383
2304	30-31	0.47316823618393045
2304	32-33	0.5251830901641128
2304	34-35	0.49763763041595155
2304	36-37	0.4835719800401179
2304	38-39	0.5120838101204939
2304	40-41	0.5483793525516916
2304	42-43	0.45626969138418616
2304	44-45	0.49282814895239113
2304	46-47	0.5093922131995043
2304	48-49	0.5222319502051533
2304	50-51	0.5080414530874648
2304	52-53	0.46662750198033365
2304	54-55	0.5019035421187041
2304	56-57	0.5367650714762817
2304	58-59	0.5213623377153525
2304	60-61	0.5266254799716279
2304	62-63	0.5805031086854981
2304	64-65	0.6074641623383172
2304	66-67	0.5687047927900579
2304	68-69	0.5739180466538869
2304	70-71	0.5685784379185321
2304	72-73	0.5616100397326278
2304	74-75	0.6353364162751802
2304	76-77	0.803599870984165
2304	78-79	0.6874584541581754
2304	80-81	0.6546040127359944
2304	82-83	0.6317006704670121
2304	84-85	0.6064690989112123
2304	86-87	0.496460950960369
2304	88-89	0.5974129012175808
2304	90-91	0.6557200253242073
2304	92-93	0.6989507507551949
2304	94-95	0.603638582598947
2304	96-97	0.5774138336884604
2304	98-99	0.671574897841758
2304	100-101	0.8057974003922475
2202	1	0.03535049476958818
2202	2	0.04315767018677974
2202	3	0.1620938860865806
2202	4	0.1261437949612585
2202	5	0.0835489174870716
2202	6	0.09065563686965561
2202	7	0.051612843238032724
2202	8	0.045396139758267395
2202	9	0.057982570009556866
2202	10-11	0.08989942355956515
2202	12-13	0.09305764192369281
2202	14-15	0.21699362369947295
2202	16-17	0.21167555324058895
2202	18-19	0.23149278631321835
2202	20-21	0.179614016624825
2202	22-23	0.2069569462765415
2202	24-25	0.2113054826683083
2202	26-27	0.19690030397944724
2202	28-29	0.20484838820215145
2202	30-31	0.22565071510391732
2202	32-33	0.23173037386128925
2202	34-35	0.24636688111012006
2202	36-37	0.26190810728197533
2202	38-39	0.23034559709521574
2202	40-41	0.21678211479478904
2202	42-43	0.22198221729056655
2202	44-45	0.17149984904299487
2202	46-47	0.21067432619383197
2202	48-49	0.2431042459972943
2202	50-51	0.25058973297651477
2202	52-53	0.2701651120360893
2202	54-55	0.2935555769814826
2202	56-57	0.2955529617817447
2202	58-59	0.2541449407333758
2202	60-61	0.22851874494133995
2202	62-63	0.2626377193351459
2202	64-65	0.30598480966882136
2202	66-67	0.2799151623360103
2202	68-69	0.21878061989457365
2202	70-71	0.19202459574970732
2202	72-73	0.2685980975236646
2202	74-75	0.29318373870123793
2202	76-77	0.2867657088114939
2202	78-79	0.3482151250901282
2202	80-81	0.3614623815012905
2202	82-83	0.259202917439076
2202	84-85	0.32547808340375894
2202	86-87	0.3260429971785044
2202	88-89	0.3842067105303002
2202	90-91	0.35870535416194116
2202	92-93	0.35497215928900516
2202	94-95	0.3764334149117161
2202	96-97	0.33779765351177105
2202	98-99	0.29037552322291305
2202	100-101	0.4350904485016649
1102	1	0.04559806489920959
1102	2	0.054507766100329036
1102	3	0.18525517879540843
1102	4	0.1600251909712611
1102	5	0.11473658020585731
1102	6	0.11573015248167451
1102	7	0.11551730822854722
1102	8	0.1218142546993235
1102	9	0.08618783411890263
1102	10-11	0.11391642802886537
1102	12-13	0.12003069631741425
1102	14-15	0.2343616101732664
1102	16-17	0.21865537542601032
1102	18-19	0.24764608756830597
1102	20-21	0.26485992986879126
1102	22-23	0.2524002297752972
1102	24-25	0.25367414162973745
1102	26-27	0.22982730288037345
1102	28-29	0.22401609538120937
1102	30-31	0.20021981422620883
1102	32-33	0.18898235444798672
1102	34-35	0.2533178438425381
1102	36-37	0.2652161226355716
1102	38-39	0.27326258123945735
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1213	78-79	-0.5691507420952391
1213	80-81	-0.5986207497009701
1213	82-83	-0.5842167595546073
1213	84-85	-0.9739122708602466
1213	86-87	-0.7531841401713777
1213	88-89	-0.8164490850713477
1213	90-91	-0.703343213662798
1213	92-93	-0.6604715225806785
1213	94-95	-0.5501820073715749
1213	96-97	-0.5225402970788835
1213	98-99	-0.4376724950778126
1213	100-101	-0.07660032095083835
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	15.0
8	217.0
9	572.0
10	664.0
11	435.0
12	326.0
13	364.0
14	745.0
15	1844.0
16	3956.0
17	6756.0
18	10015.0
19	14660.0
20	21264.0
21	29294.0
22	38919.0
23	50804.0
24	64836.0
25	82753.0
26	103282.0
27	129327.0
28	161525.0
29	202001.0
30	253106.0
31	315298.0
32	402826.0
33	538847.0
34	760529.0
35	1206571.0
36	2555370.0
37	6258629.0
38	4621292.0
39	2582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.10887754039591	12.285549610536954	11.137103737732227	54.468469111334905
2	21.97128695192469	18.90473095077319	40.30554670250122	18.818435394800908
3	24.559269136605252	23.597492597051154	23.933752541807568	27.909485724536026
4	28.25350150277814	28.893918516004764	17.07186574427065	25.780714236946444
5	29.10272029762606	29.536952631207797	21.122310978828875	20.238016092337265
6	21.17005143493479	32.28538535505173	23.46371499043758	23.080848219575902
7	18.965268666506798	14.510629314762541	41.44359304393489	25.080508974795773
8	21.18789373723418	19.3169184152179	27.78893458865113	31.706253258896794
9	22.532966778563633	19.079340564650852	30.69832854119251	27.68936411559301
10-11	25.57218062521439	28.17655158604936	20.814709412745767	25.43655837599048
12-13	23.370186620936224	22.269358276103716	27.597865570110645	26.76258953284941
14-15	24.270958446931132	24.05763833211479	25.576081464959373	26.095321755994703
16-17	25.541437231522202	24.209014912107225	24.36609642531861	25.883451431051963
18-19	25.214742777724386	24.303890835330996	24.641567499582447	25.839798887362175
20-21	25.120117428470756	24.511833936428935	24.90954687054538	25.45850176455493
22-23	25.12548749620648	24.800724521915427	24.496155356620143	25.57763262525795
24-25	25.03537002671667	24.64437214029981	24.521908704228714	25.7983491287548
26-27	24.918603658464466	24.35178197490398	24.908003621838006	25.82161074479355
28-29	25.170342431548136	24.442225337280902	24.47539049145248	25.91204173971848
30-31	25.19205960139912	24.12974751626032	24.63450812652193	26.043684755818635
32-33	25.202200845212317	24.558544418625182	24.423045624573138	25.816209111589362
34-35	25.226572879110847	24.64067544537032	24.202029072796392	25.93072260272244
36-37	25.342607823491225	24.34764537836791	24.43634596109922	25.873400837041643
38-39	25.218847298938087	24.38018115114769	24.664976140954145	25.735995408960083
40-41	25.72040861808486	24.280864716400643	24.480015351814185	25.518711313700308
42-43	25.129685557535158	24.38982555354604	24.780570533440038	25.69991835547876
44-45	25.107800191897773	24.306010940606974	24.878689908778178	25.70749895871708
46-47	25.531456330224245	24.16183228879369	24.412176940613346	25.894534440368716
48-49	25.293790759306166	24.26755497911921	24.56738846258045	25.871265798994177
50-51	25.28996554407324	24.379729951309496	24.944511848949308	25.385792655667956
52-53	25.654393968355677	24.25299655037184	24.3762440288857	25.716365452386786
54-55	25.430280989683084	24.22188314739696	24.463940171923106	25.88389569099685
56-57	25.050880798197202	24.332705993696617	24.89615079211747	25.72026241598871
58-59	25.61505796394112	24.18665338246022	24.49721891137461	25.701069742224046
60-61	25.389112720783935	24.07652249426186	24.625537762760537	25.908827022193666
62-63	25.252465080797435	24.25548074246558	24.78199381442287	25.710060362314113
64-65	25.43908277336974	24.319388363016095	24.621349572621025	25.62017929099314
66-67	25.32387187399055	24.47751979172618	24.354761214423718	25.843847119859547
68-69	25.32152263066092	24.736381732847676	24.48067812780042	25.46141750869098
70-71	25.500052253998522	24.57581281052633	24.179512651666318	25.744622283808827
72-73	25.370800693403485	24.618213741505617	24.430928932763575	25.58005663232733
74-75	25.557843862466147	24.603700921642456	24.536013549139902	25.30244166675149
76-77	25.722874755088743	24.58911287833298	24.260699236049007	25.42731313052927
78-79	25.405572864234422	24.580745031299546	24.279640633352926	25.734041471113102
80-81	25.495958987642158	24.70081468811443	24.265805748493776	25.537420575749632
82-83	25.633442868498378	24.625265289236744	24.098013792237467	25.643278050027412
84-85	25.343549707421214	24.64314049093365	24.195851551915585	25.81745824972955
86-87	25.433532767375194	25.045071375951387	24.235187994808367	25.286207861865044
88-89	25.50829599457271	24.736143551249317	24.197456400430898	25.558104053747076
90-91	25.58728909664171	24.47709241163144	24.15529558050606	25.780322911220786
92-93	25.529306644199636	24.7696540312016	24.43597539613004	25.265063928468727
94-95	25.638923661128025	24.780789146180723	24.157355333393166	25.42293185929808
96-97	25.282441459254805	24.859296377625853	24.27537718559795	25.582884977521392
98-99	25.488831630434305	24.815085311995606	24.377768353040636	25.318314704529442
100-101	25.82825146570237	24.832069943389893	23.910820159902958	25.42885843100478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.5
5	2.0
6	3.5
7	6.0
8	9.5
9	14.0
10	20.5
11	48.5
12	81.0
13	117.0
14	158.5
15	195.5
16	312.5
17	489.0
18	680.0
19	884.5
20	1236.0
21	1981.0
22	3128.5
23	4877.5
24	7136.0
25	10401.0
26	15030.5
27	21129.5
28	29644.0
29	40458.0
30	52160.0
31	66127.0
32	83846.5
33	105114.0
34	132478.5
35	173693.0
36	218091.0
37	268537.5
38	326011.0
39	380979.5
40	442130.0
41	504222.5
42	563038.5
43	606564.5
44	640743.5
45	657904.0
46	652718.0
47	654004.5
48	635962.0
49	615599.5
50	626637.0
51	611771.5
52	591463.5
53	597692.5
54	610757.5
55	627354.5
56	640550.0
57	633955.5
58	583333.5
59	509732.5
60	462772.5
61	432288.5
62	393926.5
63	351498.5
64	302061.0
65	269941.0
66	257394.5
67	246381.0
68	223061.5
69	186981.5
70	158256.5
71	132907.5
72	107604.5
73	87604.0
74	75390.0
75	61592.0
76	42501.5
77	28664.0
78	20422.0
79	17380.5
80	13178.0
81	7705.0
82	4036.0
83	1815.5
84	1027.0
85	641.5
86	452.0
87	294.0
88	186.5
89	146.0
90	112.5
91	74.5
92	46.0
93	27.0
94	13.5
95	9.0
96	6.5
97	4.0
98	1.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0788021004476215
2	0.011721097740501958
3	0.002528079904814148
4	0.0
5	0.0018386035671375622
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.408248020446169E-6
12-13	8.127973086431296E-5
14-15	2.8027493401487227E-5
16-17	9.080907862081862E-4
18-19	0.003741670369098545
20-21	0.0
22-23	0.0
24-25	8.688522954461042E-5
26-27	3.6435741421933396E-4
28-29	6.67054342955396E-4
30-31	0.01266562426813208
32-33	0.04955260833382942
34-35	0.04392468765881078
36-37	0.049622677067333136
38-39	0.04204404285157099
40-41	0.06960347711325338
42-43	0.062321934327547006
44-45	0.046284602603216006
46-47	0.03593685203938693
48-49	0.01153891903339229
50-51	0.03918243577527915
52-53	0.05862230519855069
54-55	0.05457793790071608
56-57	0.046206125621691845
58-59	0.05004869496703574
60-61	0.05132674866614356
62-63	0.06650083359370874
64-65	0.06172494871809532
66-67	0.02817884186585526
68-69	0.06700813122427567
70-71	0.07421119702845788
72-73	0.09101648207198962
74-75	0.051130556212333145
76-77	0.02861607076291846
78-79	0.0035342669179275394
80-81	0.0012472234563661818
82-83	0.003396932200260252
84-85	0.00711057507595731
86-87	8.071918099628322E-4
88-89	8.043890606226834E-4
90-91	0.0014826544009386745
92-93	8.744577941264015E-4
94-95	0.0029316758097955643
96-97	0.0
98-99	2.690639366542774E-4
100-101	3.139079260966569E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.7839626E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.827098180749516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25807962738199	32.54516146543186
2	13.38987328442593	11.736785821286604
3	4.510072141651729	5.9299012366330075
4	2.3607904884704745	4.138663860895004
5	1.2300936396340576	2.6955717357878677
6	0.8834202969317614	2.323064885309712
7	0.5533291307890871	1.6975567098953481
8	0.4567991173297291	1.6016143811271804
9	0.39697721165678784	1.5658523307722
>10	1.7182721436244526	13.569600752837596
>50	0.12771050348202345	3.8992859816505168
>100	0.09681668691989229	8.644414597974398
>500	0.010740988259580114	3.3033492828502187
>1k	0.006730743354819917	5.230581949532426
>5k	2.4272087710782884E-4	0.7050502051992865
>10k+	5.12752106397818E-5	0.4135448028166022
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT	25439	0.14259828092808674	TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA	25169	0.1410847962844064	TruSeq Adapter, Index 15 (97% over 40bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.1632265160715813	2.802749340148723E-5	0.0
2	0.16406734087362593	3.3632992081784676E-5	5.605498680297446E-6
3	0.16645528331143264	3.3632992081784676E-5	5.605498680297446E-6
4	0.1733724686829197	3.3632992081784676E-5	1.1210997360594892E-5
5	0.17456643990182305	3.923849076208212E-5	1.1210997360594892E-5
6	0.17769430816542903	5.0449488122677014E-5	1.1210997360594892E-5
7	0.17844544498858889	5.0449488122677014E-5	1.1210997360594892E-5
8	0.1803793420332915	5.0449488122677014E-5	1.1210997360594892E-5
9	0.18334465083516885	5.0449488122677014E-5	1.6816496040892338E-5
10-11	0.1882242374363678	6.16604854832719E-5	1.6816496040892338E-5
12-13	0.1931262460322879	7.006873350371807E-5	2.2421994721189784E-5
14-15	0.1989083179210147	7.847698152416423E-5	2.5224744061338507E-5
16-17	0.20400651897074523	8.408248020446168E-5	3.0830242741635957E-5
18-19	0.20846569317092187	8.68852295446104E-5	3.3632992081784676E-5
20-21	0.21243999173525274	9.529347756505658E-5	3.3632992081784676E-5
22-23	0.2160611438827249	1.0930722426580019E-4	3.6435741421933396E-5
24-25	0.21903205818328253	1.2051822162639508E-4	3.923849076208212E-5
26-27	0.22204221097460228	1.5134846436803102E-4	3.923849076208212E-5
28-29	0.2243965204203272	1.597567123884772E-4	3.923849076208212E-5
30-31	0.22667795838320826	1.7657320842936954E-4	4.484398944237957E-5
32-33	0.2287968368843607	2.1300894985130294E-4	4.764673878252829E-5
34-35	0.23059339921139604	2.2421994721189784E-4	6.726598416356935E-5
36-37	0.23223861307406332	2.2982544589219528E-4	7.567423218401552E-5
38-39	0.23401275340637748	2.3543094457249272E-4	7.847698152416424E-5
40-41	0.23573083875188863	2.438391925929389E-4	8.408248020446168E-5
42-43	0.23748535983882174	2.606556886338312E-4	8.968797888475914E-5
44-45	0.239262302920476	2.8588043269516975E-4	9.809622690520531E-5
46-47	0.2408710810417214	2.9148593137546717E-4	1.0089897624535403E-4
48-49	0.24240979042946303	2.942886807156159E-4	1.1210997360594892E-4
50-51	0.2441390867723348	3.1671067543680566E-4	1.1771547228624636E-4
52-53	0.245952465595411	3.2511892345725186E-4	1.2051822162639508E-4
54-55	0.24777144991716754	3.447381688382929E-4	1.233209709665438E-4
56-57	0.24969413596450957	3.811739102602263E-4	1.233209709665438E-4
58-59	0.251756959478859	3.895821582806725E-4	1.261237203066925E-4
60-61	0.2540243836950393	4.092014036617135E-4	1.3172921898698997E-4
62-63	0.25638990413812485	4.34426147723052E-4	1.4013746700743614E-4
64-65	0.2590020665231435	4.484398944237957E-4	1.4013746700743614E-4
66-67	0.26219439802157285	4.5404539310409315E-4	1.5134846436803102E-4
68-69	0.26522417005827365	4.6525639046468797E-4	1.5134846436803102E-4
70-71	0.26897424867539266	4.7927013716543164E-4	1.5134846436803102E-4
72-73	0.27333812939800417	4.820728865055803E-4	1.569539630483285E-4
74-75	0.2781980967538221	5.072976305669189E-4	1.569539630483285E-4
76-77	0.28408387036813443	5.269168759479599E-4	1.6255946172862593E-4
78-79	0.2909870419929207	5.269168759479599E-4	1.6536221106877466E-4
80-81	0.29990539039327396	5.493388706691496E-4	1.82178707109667E-4
82-83	0.31176662560078333	5.577471186895958E-4	2.0179795249070806E-4
84-85	0.3268145868080418	5.773663640706369E-4	2.0460070183085676E-4
86-87	0.3454332506746498	5.913801107713805E-4	2.074034511710055E-4
88-89	0.3684998777440738	6.3622410021376E-4	2.1861444853160037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAT	2180	0.0	29.861446	1
CACACGT	12175	0.0	23.00927	12-13
TATGCCG	10585	0.0	23.005783	48-49
CGTATGC	10760	0.0	22.981764	46-47
CTCGTAT	11005	0.0	22.643127	44-45
ACATGCG	4265	0.0	20.815973	4
CATGCGT	4260	0.0	20.72896	5
CGTCTGA	14160	0.0	19.716658	16-17
GCGTATT	3125	0.0	18.840801	2
AGCACAC	15375	0.0	18.388369	10-11
AGTCACA	15260	0.0	18.322386	28-29
ACATGTC	15645	0.0	18.02463	32-33
CACGTCT	16230	0.0	17.245846	14-15
TAAGTCG	3135	0.0	17.112514	9
TCCGTAC	4375	0.0	16.928585	5
ATGTCAG	16820	0.0	16.738237	34-35
TCTGAAC	17145	0.0	16.421425	18-19
TCACATG	17320	0.0	16.212198	30-31
CCAGTCA	18190	0.0	15.552761	26-27
CTGACAT	10495	0.0	15.076778	1
>>END_MODULE